Induction of secretory and tumoricidal activities in peritoneal macrophages by ginsan

Song, Jie‐Young; Han, Seon-Kyu; Son, Eun-Hwa; Pyo, Suhkneung; Yun, Yeon-Sook; Yi, Seh-Yoon

doi:10.1016/s1567-5769(01)00211-9

Cited by 109 publications

(89 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ginsan, a polysaccharide, was purified from the ethanolinsoluble fraction of the aqueous Panax ginseng extract, as described previously [12]. Further purification was carried out successively using size exclusion and ion exchange column chromatography, and NMR analysis revealed that ginsan was composed of a(1?6)glucopyranoside and b(2?6) fructofuranoside in a 5:2 molar ratio.…”

Section: Preparation Of Ginsanmentioning

confidence: 99%

“…We have previously reported that ginsan functions as an effective biological response modifier; ginsan stimulates NK and T cells, produces a variety of cytokines, and induces tumoricidal and antimicrobial activities in macrophages [12]. Furthermore, we have demonstrated that ginsan possesses potent anti-septicemic activity by producing nitric oxide via proinflammatory cytokine production in stimulated macrophages in vitro [13].…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

The immunomodulator ginsan induces resistance to experimental sepsis by inhibiting Toll‐like receptor‐mediated inflammatory signals

et al. 2005

Self Cite

View full text Add to dashboard Cite

Ginsan, a polysaccharide extracted from Panax ginseng, has multiple immunomodulatory effects. In this study, we show that pretreatment of ginsan (25 lg/kg) protected mice from lethality induced by Staphylococcus aureus challenge. This survival benefit was associated with enhanced bacterial clearance from circulation, spleen and kidney. The phagocytic activity of macrophages treated with ginsan was significantly enhanced against S. aureus. However, the production of proinflammatory cytokines, such as TNFa, IL-1b, IL-6, IFN-c, IL-12, and IL-18, was markedly down-regulated in ginsan-treated mice compared with those of control-infected mice. The expression of Toll-like receptor (TLR) 2 and the adaptor molecule MyD88, which was greatly increased in septic macrophages, was significantly reduced by ginsan treatment in vitro. Similarly, the expression of phospho-JNK1/2, phospho-p38 MAPK, and NF-jB was decreased in the same culture system. These results illustrate that the antiseptic activity of ginsan can be attributed to enhanced bacterial clearance, and reduced proinflammatory cytokines via the TLR signaling pathway.

show abstract

Section: Preparation Of Ginsanmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

The immunomodulator ginsan induces resistance to experimental sepsis by inhibiting Toll‐like receptor‐mediated inflammatory signals

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…Polysaccharides from plant sources with immunomodulatory, anti-tumor, anti-viral, anti-bacterial, anti-inflammatory, anti-oxidant, and anti-diabetogenic activities have been reported [2,[9][10][11][12][13][14][15]. And a few polysaccharides, including lentinan, Astragalus polysaccharide, polyporus polysaccharide and Achyranthes bidentata polysaccharide have been licensed for clinical application in China [16].…”

Section: Discussionmentioning

confidence: 99%

“…This activity may be relevant to clinical application involving bacterial infection and toxemia. Challenges in development of botanical polysaccharides for clinical application include lack of methodology for structure identification and characterization, isolation and purification, and product quality control, has been recognized [11]. Our research will focus on the application of new methodologies to characterize the polysaccharide structures from ginseng and to elucidate the structurebiological activity relationship.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Polysaccharides of American Ginseng Panax quinquefolius L. in Modulation of Immune Function: Phytochemical and Pharmacological Characterization

Lui¹,

Chike²,

Jose³

et al. 2012

The Complex World of Polysaccharides

View full text Add to dashboard Cite

“…Ginsan functions as an effective biological response modifier; it stimulates NK and T cells, stimulates the production of various cytokines, and induces tumoricidal and antimicrobial activities in macrophages (5).…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Ginsan Against Vibrio vulnificus Infection

Lim

Yun

et al. 2009

J Bacteriol Virol

View full text Add to dashboard Cite

Ginsan, a botanic polysaccharide extracted from Panax ginseng, has recently been reported to modulate mucosal immune response. In this study, we investigated the protective effect of Ginsan against fatal Vibrio vulnificus mucosal infection. A lethal dose of V. vulnificus (1.0 × 10 6 CFU/mouse) was nasally inoculated to mice. The bacterial count in the nasal associated lymphoid tissue (NALT) of the mouse was significantly reduced in the Ginsan-treated group. The Ginsan-treated group showed improved survival compared to the control group (100% vs 18%). To elucidate the effect of Ginsan on modulating host immune response, cytokine mRNA expressions involved in mediating inflammation were determined by semiquantitative RT-PCR in the NALTs of the infected mice. Most of the cytokine mRNAs were similarly expressed as the control group. However, COX-1 mRNA expression level was higher in Ginsan-treated group compared to the control group. The protective effect of Ginsan was antagonized by treating with a specific COX-1 inhibitor, SC-560. Thus, these data suggest that the protective effect of Ginsan against V. vulnificus infection is partly mediated by modulating COX-1 expression.

show abstract

Induction of secretory and tumoricidal activities in peritoneal macrophages by ginsan

Cited by 109 publications

References 13 publications

The immunomodulator ginsan induces resistance to experimental sepsis by inhibiting Toll‐like receptor‐mediated inflammatory signals

The immunomodulator ginsan induces resistance to experimental sepsis by inhibiting Toll‐like receptor‐mediated inflammatory signals

Bioactive Polysaccharides of American Ginseng Panax quinquefolius L. in Modulation of Immune Function: Phytochemical and Pharmacological Characterization

Protective Effect of Ginsan Against Vibrio vulnificus Infection

Contact Info

Product

Resources

About