Induction of somatic embryogenesis in cultured cells of Chenopodium quinoa

Eisa, S. S.; Koyro, Hans‐Werner; Kögel, Karl-Heinz; Imani, Jafargholi

doi:10.1007/s11240-004-4793-z

Cited by 21 publications

(15 citation statements)

References 19 publications

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“…Callus formed on MS and B5 media with the presence of 2,4D+0,2 mg/l BA have significant development of diameter and they are friable, similar results have been demonstrated by the work of EISA et al [24] on quinoa. Callus formed with the presence of ANA are smaller and compact.…”

Section: The Effect Of Medium and Growth Regulators On Callogenesissupporting

confidence: 85%

Influence of medium and growth regulators on callogenesis of quinoa (Chenopodium quinoa Willd.) and effect of hydrous stress induced by P.E.G 6000 on the callus

Telahigue

Toumi²

2017

HBR

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The induction and growth of quinoa's callus depend on several factors, including the culture medium and the nature of the growth hormone and its dose. In effect, the best callogenesis rates were obtained with the media MS and B5 with respect to the media WHITE and KNOP the callogenesis is too low or zero. The best combination used was 0.2 BA+2.4 D give well-developed callus. To obtain water-stress resistant cell lines, the effect of water stress induced by polyethylene glycol (P. E. G 6000) on the growth, osmotic potential and metabolic parameter of Chenopodium quinoa callus was studied. Applied water stress showed a reduction in the growth of stressed callus compared to the control. The presence of PEG in the culture medium caused a decrease in the content of fresh matter as well as the dry matter content compared to the control. Water stress also significantly affected the water parameters of calluses. The chlorophyll a, b and carotenoids content decreased, but this decrease is not too pronounced.

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Section: The Effect Of Medium and Growth Regulators On Callogenesissupporting

confidence: 85%

Influence of medium and growth regulators on callogenesis of quinoa (Chenopodium quinoa Willd.) and effect of hydrous stress induced by P.E.G 6000 on the callus

Telahigue

Toumi²

2017

HBR

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“…Most studies were conducted on Beta vulgaris (Taghipour et al, 2013;Yildiz et al, 2013), Spinacia (Al-Khayri et al, 1991;Shojaei et al, 2010;Usman et al, 2014), Atriplex (Mei et al, 1997;Nedjimi et al, 2006), and Chenopodium sp. (Burnouf-Radosevich and Paupardin, 1985;Eisa et al, 2005;Hesami and Daneshvar, 2016a).…”

Section: Introductionmentioning

confidence: 99%

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

Hesami

Naderi

Yoosefzadeh-Najafabadi

2018

bta

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Chenopodium quinoa Willd. is known as a multipurpose crop that could be employed as an alternative on unsuitable lands with limited water resources such as arid and semi-arid regions. However, the biggest problem in cultivating this plant is contamination by viruses that are carried by quinoa seeds and cause diseases. Therefore, in vitro propagation methods could be applied to produce virus-free plants. The capacity of sodium hypochlorite to sterilize seeds and the regeneration potential of cotyledonary node segments were studied on Murashige and Skoog (MS) medium consisting of various concentrations of cytokinins and auxins either alone or in combinations. Although no contamination was observed after sterilization with 20% sodium hypochlorite and 10 and 15 min of immersion; with 25% sodium hypochlorite and 5, 10, and 15 min of immersion, the highest seed germination (100%) was obtained after sterilization with 20% sodium hypochlorite for 5 minutes. MS medium supplemented with 2.0 mg/l 6-benzylaminopurine (BAP) had the highest shoot induction frequency (93.33%) with an average of 4.96 shoots per node explant. This study is the first report of an appropriate and effective in vitro regeneration protocol for Chenopodium quinoa via direct organogenesis through cotyledonary node explants derived from axenic-seedlings, which can be effectively employed for the genetic manipulations and exploitation of active biomolecules of this valuable plant. This protocol can also increase the speed of quinoa breeding programs.

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“…Chenopodium quinoa Willd. is a facultative halophyte and could be used as a promising alternative cash crop for land and water unsuitable for conventional crops in arid and semiarid regions (Eisa et al 2005). During the recent Arab Univ.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Some Chenopodium Quinoa Cultivars Under Saline Soil Conditions in Egypt

Ebrahim

Abdel-Ati

Hussin

et al. 2018

Arab Universities Journal of Agricultural Sciences

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This study aimed to evaluate seed yield, morphological variability and nutritional quality for two cultivars of Chenopodium quinoa under high saline soil conditions (ECe 22 dSm-1) in Egyptian Northeastern coast. Responses to salinity were greatly differed between the two cultivars. The Peruvian cultivar CICA produced seed yield significantly higher than Bolivian cultivar Real. CICA cultivar also showed significant high performances for most of morphological traits. Among the 10 morphological traits, leaves dry weight, shoot fresh weight and leaves fresh weight showed significant positive association with seed yield. No significant difference has been found between both cultivars for most seed quality traits except for the concentration of crude protein and crude fiber in seeds were significantly higher in CICA cultivar. Although CICA cultivar exhibited significantly higher sodium concentration in the leaves than that found in the leaves of Real cultivar, but it was much more efficient in restricting sodium uploading into seed. These results revealed that the Peruvian cultivar CICA seems to be adaptable and more suited to dry-saline soil in Northeastern coastal region of Egypt, as it gave considerable high seed yield with better quality in terms of high protein and fiber percentage and low Na concentration in seeds.

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Induction of somatic embryogenesis in cultured cells of Chenopodium quinoa

Cited by 21 publications

References 19 publications

Influence of medium and growth regulators on callogenesis of quinoa (Chenopodium quinoa Willd.) and effect of hydrous stress induced by P.E.G 6000 on the callus

Influence of medium and growth regulators on callogenesis of quinoa (Chenopodium quinoa Willd.) and effect of hydrous stress induced by P.E.G 6000 on the callus

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

Evaluation of Some Chenopodium Quinoa Cultivars Under Saline Soil Conditions in Egypt

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