Induction of Taxol biosynthesis by Aspergillus terreus, endophyte of Podocarpus gracilior Pilger, upon intimate interaction with the plant endogenous microbes

El-Sayed, Ashraf S. A.; Safan, Samia; Mohamed, Nabil Z.; Shaban, Lamis; Ali, Gul Shad; Sitohy, Mahmoud

doi:10.1016/j.procbio.2018.04.020

Cited by 56 publications

(91 citation statements)

References 47 publications

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“…Fungal endophytes elevated the hope for mass production of Taxol due to their fast growth, feasibility of fermentation, genetic manipulation, and independence of climatic changes [20]. Nevertheless, the anticipation of fungi for commercial Taxol production has been challenged by their lower yield and drastic loss of Taxol yield with sub-culturing and storage [5,6,8,13,21]. Thus, exploring novel fungal isolates with Taxol-producing potency was the objective.…”

Section: Taxol Production By the Selected Fungal Isolates In Responsementioning

confidence: 99%

“…Taxomyces andreanae was the first recognized Taxol-producing endophyte of Taxus spp [14], after which an unlimited number of studies became motivated to isolate endophytic fungi with Taxol metabolic-producing potency [5][6][7][8]. Nevertheless, the implementation of these fungi for commercial production of Taxol has been confronted by the loss of Taxol productivity with subculturing and storage [1,6]. Manipulating the Taxol yield by specific inhibitors to modulate the metabolic yield by endophytic fungi was emphasized in our previous study [1].…”

Section: Introductionmentioning

confidence: 99%

“…The anticancer activity of Taxol has been emphasized towards various tumor cell lines [3], including leukemia, breast, ovarian, and lung cancers [2,4], as well as against polycystic kidney diseases. Taxol production from different sources and their challenges have been addressed extensively on our studies [1,5,6]. Taxol is now being commercially produced by a semi-synthetic method, starting with 10-deacetylbaccatin III [7] Molecules 2020, 25, 263 2 of 13 extracted from the needles of T. baccata and T. walichiana growing in Europe and Asia.…”

Section: Introductionmentioning

confidence: 99%

“…Deciphering the potency of endophytic fungi for Taxol production opened a new avenue for commercial Taxol production due to their rapid growth, independence on climatic changes, and feasibility of genetic manipulation [5,13]. Taxomyces andreanae was the first recognized Taxol-producing endophyte of Taxus spp [14], after which an unlimited number of studies became motivated to isolate endophytic fungi with Taxol metabolic-producing potency [5][6][7][8]. Nevertheless, the implementation of these fungi for commercial production of Taxol has been confronted by the loss of Taxol productivity with subculturing and storage [1,6].…”

Section: Introductionmentioning

confidence: 99%

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Conjugation of Aspergillus flavipes Taxol with Porphyrin Increases the Anticancer Activity of Taxol and Ameliorates Its Cytotoxic Effects

et al. 2020

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Taxol is one of the potential anticancer drugs; however, the yield of Taxol and its cytotoxicity are common challenges. Thus, manipulating the Taxol biosynthetic pathway from endophytic fungi, in addition to chemical modification with biocompatible polymers, is the challenge. Four fungal isolates, namely, Aspergillus flavipes, A. terreus, A. flavus, and A. parasiticus, were selected from our previous study as potential Taxol producers, and their potency for Taxol production was evaluated in response to fluconazole and silver nitrate. A higher Taxol yield was reported in the cultures of A. flavipes (185 µg/L) and A. terreus (66 µg/L). With addition of fluconazole, the yield of Taxol was increased 1.8 and 1.2-fold for A. flavipes and A. terreus, respectively, confirming the inhibition of sterol biosynthesis and redirecting the geranyl phosphate pool to terpenoids synthesis. A significant inhibition of ergosterol biosynthesis by A. flavipes with addition of fluconazole was observed, correlating with the increase on Taxol yield. To increase the Taxol solubility and to reduce its cytotoxicity, Taxol was modified via chemical conjugation with porphyrin, and the degree of conjugation was checked from the Thin layer chromatography and UV spectral analysis. The antiproliferative activity of native and modified Taxol conjugates was evaluated; upon porphyrin conjugation, the activity of Taxol towards HepG2 was increased 1.5-fold, while its cytotoxicity to VERO cells was reduced 3-fold.

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Section: Taxol Production By the Selected Fungal Isolates In Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Conjugation of Aspergillus flavipes Taxol with Porphyrin Increases the Anticancer Activity of Taxol and Ameliorates Its Cytotoxic Effects

et al. 2020

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“…In 2018, El-Sayed et al [28] reported that three endophytes identified as Aspergillus terreus, recovered from cork, leaves, and twigs of P. graciliors, produced taxol. Interestingly, when growing fungal cultures were treated with surface sterilized leaves, taxol production increased by 2-to 3-fold.…”

Section: Non-taxaceae Sources Of Taxolmentioning

confidence: 99%

Plant Endophytes and Epiphytes: Burgeoning Sources of Known and “Unknown” Cytotoxic and Antibiotic Agents?

Newman¹,

Cragg²

2020

Planta Med

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In the last 20 or so years, the influence of endophytes and, quite recently, epiphytes of plants upon the compounds found in those plants, which were usually assumed to be phytochemicals produced by the plant for a variety of reasons, often as a defense against predators, is becoming more evident, in particular in the case of antitumor agents originally isolated from plant sources, though antibiotic agents might also be found, particularly from epiphytes. In this review, we started with the first report in 1993 of a taxol-producing endophyte and then expanded the compounds discussed to include camptothecin, the vinca alkaloids, podophyllotoxin, and homoharringtonine from endophytic microbes and then the realization that maytansine is not a plant secondary metabolite at all, and that even such a well-studied plant such as Arabidopsis thaliana has a vast repertoire of potential bioactive agents in its leaf epiphytic bacteria. We have taken data from a variety of sources, including a reasonable history of these discoveries that were not given in recent papers by us, nor in other papers covering this topic. The sources included the Scopus database, but we also performed other searches using bibliographic tools, thus, the majority of the papers referenced are the originals, though we note some very recent papers that have built on previous results. We concluded with a discussion of the more modern techniques that can be utilized to “persuade” endophytes and epiphytes to switch on silent biosynthetic pathways and how current analytical techniques may aid in evaluating such programs. We also comment at times on some findings, particularly in the case of homoharringtonine, where there are repetitious data reports differing by a few years claiming the same endophyte as the producer.

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Pharmaceutical Potential of Marine Fungal Endophytes

Jeewon¹,

Luckhun²,

Bhoyroo³

et al. 2019

Bioactive Molecules in Food

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Induction of Taxol biosynthesis by Aspergillus terreus, endophyte of Podocarpus gracilior Pilger, upon intimate interaction with the plant endogenous microbes

Cited by 56 publications

References 47 publications

Conjugation of Aspergillus flavipes Taxol with Porphyrin Increases the Anticancer Activity of Taxol and Ameliorates Its Cytotoxic Effects

Conjugation of Aspergillus flavipes Taxol with Porphyrin Increases the Anticancer Activity of Taxol and Ameliorates Its Cytotoxic Effects

Plant Endophytes and Epiphytes: Burgeoning Sources of Known and “Unknown” Cytotoxic and Antibiotic Agents?

Pharmaceutical Potential of Marine Fungal Endophytes

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