Induction of Tetraploidy to Feverfew (Tanacetum parthenium Schulz-Bip.): Morphological, Physiological, Cytological, and Phytochemical Changes

Majdi, Mohammad; Karimzadeh, Ghasem; Malboobi, Mohammad Ali; Omidbaigi, R.; Mirzaghaderi, Ghader

doi:10.21273/hortsci.45.1.16

Cited by 82 publications

(46 citation statements)

References 27 publications

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“…Our analysis indicates that chromosome doubling has a significant influence on secondary metabolite concentrations. Similar results have been reported for other plant species (Banyai et al, 2010;Majdi et al, 2010;Mishra et al, 2010;Caruso et al, 2011;Dehghan et al, 2012;Abdoli et al, 2013;Lavania, 2013;Dixit and Chaudhary, 2014). Phenolic compounds are active in pathogen resistance, pigment production, and protection against ultraviolet radiation (Winkel-Shirley, 2001); they also qualitatively and quantitatively improve the biochemical profile of plants (Maruška et al, 2010).…”

Section: Phenolic Compounds In Triploid and Hexaploid M × Giganteussupporting

confidence: 80%

Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Ghimire

Seong

Nguyen

et al. 2016

Industrial Crops and Products

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Section: Phenolic Compounds In Triploid and Hexaploid M × Giganteussupporting

confidence: 80%

Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Ghimire

Seong

Nguyen

et al. 2016

Industrial Crops and Products

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“…The enlarged stomata and pollen grains of induced doubled haploid plants reported in our study have alson bee observed in other plants (Takamura and Miyajima 1996;Thao et al 2003;Majdi et al 2010). In the present study, an increased ploidy level was correlated with a larger size of the guard cells and a lower density of leaf stomata.…”

Section: Discussionsupporting

confidence: 89%

Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)

Mohammadi

Moieni

Ebrahimi

et al. 2011

Plant Cell Tiss Organ Cult

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An efficient method for producing doubled haploid plants of oilseed rape (Brassica napus L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12, 24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment). The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient chromosome doubling for the production of doubled haploid lines of oilseed rape.

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“…The mobile phase was an isocratic 55 % acetonitrile: 45 % water at 1.5 ml/min flow rate for 5 min. The UV detector was set at 210 nm (Majdi et al 2010).…”

Section: Quantification Of Parthenolidementioning

confidence: 99%

Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

2015

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Up-regulation of germacrene A synthase and down-regulation of parthenolide hydroxylase genes play key role in parthenolide accumulation of feverfew plants treated with methyl jasmonate and salicylic acid. Parthenolide is an important sesquiterpene lactone due to its anti-migraine and anti-cancer properties. Parthenolide amount was quantified by high-performance liquid chromatography after foliar application of methyl jasmonate (100 µM) or salicylic acid (1.0 mM) on feverfew leaves in time course experiment (3-96 h). Results indicate that exogenous application of methyl jasmonate or salicylic acid activated parthenolide biosynthesis. Parthenolide content reached its highest amount at 24 h after methyl jasmonate or salicylic acid treatments, which were 3.1- and 1.96-fold higher than control plants, respectively. Parthenolide transiently increased due to methyl jasmonate or salicylic acid treatments until 24 h, but did not show significant difference compared with control plants at 48 and 96 h time points in both treatments. Also, the transcript levels of early pathway (upstream) genes of terpene biosynthesis including 3-hydroxy-3-methylglutaryl-coenzyme A reductase, 1-deoxy-D-xylulose-5-phosphate reductoisomerase and hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase and the biosynthetic genes of parthenolide including germacrene A synthase, germacrene A oxidase, costunolide synthase and parthenolide synthase were increased by methyl jasmonate and salicylic acid treatments, but with different intensity. The transcriptional levels of these genes were higher in methyl jasmonate-treated plants than salicylic acid-treated plants. Parthenolide content measurements along with expression pattern analysis of the aforementioned genes and parthenolide hydroxylase as side branch gene of parthenolide suggest that the expression patterns of early pathway genes were not directly consistent with parthenolide accumulation pattern; hence, parthenolide accumulation is probably further modulated by the expression of its biosynthetic genes, especially germacrene A synthase and also its side branch gene, parthenolide hydroxylase.

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Induction of Tetraploidy to Feverfew (Tanacetum parthenium Schulz-Bip.): Morphological, Physiological, Cytological, and Phytochemical Changes

Cited by 82 publications

References 27 publications

Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)

Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

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