Induction of the cytoplasmic ‘petite’ mutation by chemical and physical agents in Saccharomyces cerevisiae

Ferguson, Lynnette R.; Borstel, R. C. von

doi:10.1016/0027-5107(92)90042-z

Cited by 86 publications

(36 citation statements)

References 190 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ROS damage mitochondria and damaged mitochondria produce more ROS, and finally, overproduced ROS cause mtDNA deletion. Ultimately, cells with mtDNA deletion generate small colonies even on fermentable medium, and this phenotype is called "petite" (20). From these findings, we conclude that mitophagy prevents mtDNA deletion by eliminating ROS-producing mitochondria during nitrogen starvation.…”

mentioning

confidence: 83%

Mitophagy Plays an Essential Role in Reducing Mitochondrial Production of Reactive Oxygen Species and Mutation of Mitochondrial DNA by Maintaining Mitochondrial Quantity and Quality in Yeast

Kurihara

Kanki

Aoki

et al. 2012

Journal of Biological Chemistry

234

185

View full text Add to dashboard Cite

Background:The physiological importance of mitophagy in yeast has been largely unexplored. Results: Mitochondrial DNA deletion frequently occurs in mitophagy-deficient cells during nitrogen starvation because of overproduction of the reactive oxygen species from unregulated mitochondria. Conclusion: Mitophagy prevents excess reactive oxygen species production and mitochondrial DNA mutation. Significance: Our findings provide insight into mitophagy-related disorders such as Parkinson disease.

show abstract

mentioning

confidence: 83%

Mitophagy Plays an Essential Role in Reducing Mitochondrial Production of Reactive Oxygen Species and Mutation of Mitochondrial DNA by Maintaining Mitochondrial Quantity and Quality in Yeast

Kurihara

Kanki

Aoki

et al. 2012

Journal of Biological Chemistry

234

185

View full text Add to dashboard Cite

show abstract

“…Estimates of the start of the Vendian period vary from about 0.8 to more than 1·BYA. Yeasts belong to the Fungi, and are all facultative anaerobes capable of growth with or without functional mitochondria (Ferguson and von Borstel., 1992).…”

Section: Eukaryotic Glycolytic Genesmentioning

confidence: 99%

Evolution of the coordinate regulation of glycolytic enzyme genes by hypoxia

Webster

2003

Journal of Experimental Biology

149

107

View full text Add to dashboard Cite

SUMMARYTwo billion years of aerobic evolution have resulted in mammalian cells and tissues that are extremely oxygen-dependent. Exposure to oxygen tensions outside the relatively narrow physiological range results in cellular stress and toxicity. Consequently, hypoxia features prominently in many human diseases, particularly those associated with blood and vascular disorders,including all forms of anemia and ischemia. Bioenergetic enzymes have evolved both acute and chronic oxygen sensing mechanisms to buffer changes of oxygen tension; at normal PO oxidative phosphorylation is the principal energy supply for eukaryotic cells, but when the PO falls below a critical mark metabolic switches turn off mitochondrial electron transport and activate anaerobic glycolysis. Without this switch cells would suffer an immediate energy deficit and death at low PO. An intriguing feature of the switching is that the same conditions that regulate energy metabolism also regulate bioenergetic genes, so that enzyme activity and transcription are regulated simultaneously,albeit with different time courses and signaling pathways. In this review we explore the pathways mediating hypoxia-regulated glycolytic enzyme gene expression, focusing on their atavistic traits and evolution.

show abstract

“…A wide range of chemical compounds have been described as inducers of the petite phenotype in S. cerevisiae (9). Most of them are effective petite mutagens on growing yeasts, inducing partial or total loss of the mitochondrial genome by intercalating into the mtDNA.…”

Section: Influence Of Respiratory Chain Inhibitors (I) Oxygen Consummentioning

confidence: 99%

Relationships between Respiration and Susceptibility to Azole Antifungals in Candida glabrata

Brun¹,

Aubry²,

Lima

et al. 2003

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Over the past two decades, the incidence of infections due to Candida glabrata, a yeast with intrinsic low susceptibility to azole antifungals, has increased markedly. Respiratory deficiency due to mutations in mitochondrial DNA (mtDNA) associated with resistance to azoles frequently occurs in vitro in this species. In order to specify the relationships between respiration and azole susceptibility, the effects of respiratory chain inhibitors on a wild-type isolate of C. glabrata were evaluated. Respiration of blastoconidia was immediately blocked after extemporaneous addition of potassium cyanide, whereas a 4-h preincubation was required for sodium azide. Antifungal susceptibility determined by a disk diffusion method on Casitone agar containing sodium azide showed a significant decrease in the susceptibility to azoles. Biweekly subculturing on Casitone agar supplemented with sodium azide was therefore performed. This resulted after 40 passages in the isolation of a respiration-deficient mutant, as suggested by its lack of growth on glycerol-containing agar. This respiratory deficiency was confirmed by flow cytometric analysis of blastoconidia stained with rhodamine 123 and by oxygraphy. Moreover, transmission electron microscopy and restriction endonuclease analysis of the mtDNA of mutant cells demonstrated the mitochondrial origin of the respiratory deficiency. Finally, this mutant exhibited cross-resistance to all the azoles tested. In conclusion, blockage of respiration in C. glabrata induces decreased susceptibility to azoles, culminating in azole resistance due to the deletion of mtDNA. This mechanism could explain the induction of petite mutations by azole antifungals which have been demonstrated to act directly on the mitochondrial respiratory chain.

show abstract

Induction of the cytoplasmic ‘petite’ mutation by chemical and physical agents in Saccharomyces cerevisiae

Cited by 86 publications

References 190 publications

Mitophagy Plays an Essential Role in Reducing Mitochondrial Production of Reactive Oxygen Species and Mutation of Mitochondrial DNA by Maintaining Mitochondrial Quantity and Quality in Yeast

Mitophagy Plays an Essential Role in Reducing Mitochondrial Production of Reactive Oxygen Species and Mutation of Mitochondrial DNA by Maintaining Mitochondrial Quantity and Quality in Yeast

Evolution of the coordinate regulation of glycolytic enzyme genes by hypoxia

Relationships between Respiration and Susceptibility to Azole Antifungals in Candida glabrata

Contact Info

Product

Resources

About