INDUCTION OF THE METHIONINE-ACTIVATING ENZYME IN
            <i>SACCHAROMYCES CEREVISIAE</i>

Pigg, C. JoAnne; Sorsoli, W. A.; Parks, Leo W.

doi:10.1128/jb.87.4.920-923.1964

Cited by 13 publications

(7 citation statements)

References 15 publications

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“…The enzyme has been monitored in a variety of microorganisms (8,17,21), as well as in liver extracts (6,18). Under certain conditions, methionine adenosyltransferase from yeast has been shown to be induced in the presence of low concentrations of methionine (20).…”

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confidence: 99%

Methionine Adenosyltransferase and Ethionine Resistance in Saccharomyces cerevisiae

Mertz

Spence

1972

J Bacteriol

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The methionine adenosyltransferase is repressed in Saccharomyces ,cerevisiae during growth in the presence of excess methionine. The relationship of this repression to the level of intracellular S-adenosylmethionine is discussed. In conjunction with these studies, an ethionine-resistant mutant has been investigated which has a low level of methionine adenosyltransferase under all conditions tested. The mechanism of ethionine resistance in the latter strain apparently depends on its inability to form large quantities of intracellular S-adenosylethionine. With respect to the methionine adenosyltransferase, there is no apparent interaction between ethionine-resistant and ethionine-sensitive alleles when both are present in the heterozygous diploid.Recent studies performed to establish the mechanism of ethionine resistance in this strain strongly suggest that the mutation is a regulatory alteration in S-adenosylmethionine synthesis. These studies, together with the regulatory aspects of S-adenosylmethionine synthesis, are the subject of this report.

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confidence: 99%

Methionine Adenosyltransferase and Ethionine Resistance in Saccharomyces cerevisiae

Mertz

Spence

1972

J Bacteriol

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“…Adenosylmethionine synthesis and turnover. Since methionine stimulates the formation of S-adenosylmethionine in S. cerevisiae (7,9), yet does not adversely affect the sai-1 strains, it was suggested that the mutants may employ a mechanism which limits the rate of S-adenosylmethionine synthesis, or in some other manner limits the accumulation of this compound, thereby avoiding self-inhibition. It was also possible that the sai-1 mutants might resist the effects of S-adenosylmethionine synthesized intracellularly by depositing it in the vacuole, as previously described (8,16,17).…”

Section: Resultsmentioning

confidence: 99%

“…We have been unable to distinguish sai-1 strains from wild-type strains on the basis of growth in the presence of methionine alone even under conditions where the external methionine concentration was as much as 10 times higher than the concentrations reported here. One would expect an increase in the concentration of S-adenosylmethionine when the level of extracellular methionine is increased (7)(8)(9). The possibility that the externally supplied and internally synthesized S-adenosylmethionine are physically separated and participate in different reactions cannot be excluded at this time.…”

Section: Resultsmentioning

confidence: 99%

sai-1 Mutation in Saccharomyces cerevisiae : Characteristics of Inhibition by S -Adenosylmethionine and S -Adenosylhomocysteine and Protection by Methionine

1972

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The relationship of methionine to the inhibition caused by S-adenosylmethionine and S-adenosylhomocysteine in strains containing the sai-1 mutation has been investigated and shown to affect indirectly the survival of the mutants. The ability of the mutants to take up both inhibitors is similar to that of the wild-type cells. The mutant also retains the ability to hydrolyze S-adenosylhomocysteine and incorporate the hydrolytic products into the various cellular fractions. Maximal inhibition of the sai-I mutants occurs at an extracellular concentration of 0.005 mm S-adenosylmethionine and 0.025 to 0.05 mm Sadenosylhomocysteine when the cellular concentration is 0.05 mg (dry weight) per ml. The results suggest that the sai-1 mutation affects reaction(s) either not associated with methionine biosynthesis, or methionine synthesis and at least one other critical cellular function. 1050

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“…Methionine is accumulated in the sensitive strain. The measurement of accumulated methionine is complicated by the cellular conversion of the amino acid to S-adenosyl-methionine (Schlenk and De Palma, 1957; Pigg, Sorsoli, and Parks, 1964). The sulfonium derivative would not be assayed by the microbiological technique (Pigg, Spence, and Parks, 1962).…”

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confidence: 99%

AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE

1964

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acid accumulation in ethionine-resistant Saccharomyces cerevisiae. J. Bacteriol. 88:20-24. 1964.-Recessive ethionine-resistant strains of Saccharomyces cerevisiae possess a genetic lesion affecting the concentration of amino acids nonspecifically in the expandable pool of the organism. This area, which may be linked to a methionine gene, is highly mutable and accounts for resistance to certain amino acid analogues. Other mutations result in ethionine resistance by a mechanism unrelated to the amino acid uptake system.

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INDUCTION OF THE METHIONINE-ACTIVATING ENZYME IN SACCHAROMYCES CEREVISIAE

Cited by 13 publications

References 15 publications

Methionine Adenosyltransferase and Ethionine Resistance in Saccharomyces cerevisiae

Methionine Adenosyltransferase and Ethionine Resistance in Saccharomyces cerevisiae

sai-1 Mutation in Saccharomyces cerevisiae : Characteristics of Inhibition by S -Adenosylmethionine and S -Adenosylhomocysteine and Protection by Methionine

AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE

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