Induction of the skin endogenous protective mitochondrial MnSOD by<i>Vitreoscilla filiformis</i>extract

Mahé, Yann F.; Martin, Richard J.; Aubert, L; Billoni, Nelly; Collin, Christine; Pruche, Francis; Bastien, Philippe; Drost, Sharon S.; Lane, Alfred T.; Meybeck, Alain

doi:10.1111/j.1467-2494.2006.00333.x

Cited by 20 publications

(26 citation statements)

References 16 publications

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“…PROPAGAPATH allowed us to identify this pathway for the first time in reconstructed skin, with a minimal number of steps when compared with the canonical myeloid differentiation primary response gene (88), nuclear factor kappa-light-chain-enhancer of activated B cells pathway. The VFB-derived LPS fraction stimulated gene expression and was inhibited with a PKCz inhibitor, suggesting that this specific fraction is indeed a key component of the protective action of VFB on skin, as has already been shown 4,5…”

Section: Discussionsupporting

confidence: 55%

“…Both in vitro and in vivo testing have shown that VFB stimulates endogenous anti-oxidant defenses in skin: in vitro, it stimulated mitochondrial superoxide dismutase 2 (SOD2) activity3 both at the mRNA and protein levels, while, in vivo, it decreased the number of ultraviolet-induced sunburn cells in human skin. VFB was thus proposed as an effective anti-aging ingredient for skin improvement and protection from endogenous and exogenous oxidative stress 3…”

Section: Introductionmentioning

confidence: 99%

“…This Gram-negative bacterium contains a lipopolysaccharide4 (LPS), which reproduces VFB activity once isolated 5. Interestingly, it not only stimulates endogenous mitochondrial antioxidant defenses but also endogenous antimicrobial defenses such as β defensins and S100 calcium-binding protein A7 (S100A7).…”

Section: Introductionmentioning

confidence: 99%

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A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

Mahé

Perez²,

Tacheau³

et al. 2013

CCID

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Vitreoscilla filiformis (VF) biomass (VFB) has been widely used in cosmetic preparations and shown to modulate the major inducible free-radical scavenger mitochondrial superoxide dismutase in skin cells. By adding La Roche-Posay (LRP) thermal spring water to the VF culture medium, we obtained a biomass (LRP-VFB) with a similar mitochondrial superoxide dismutase activation capacity to VF. Also, the new biomass more powerfully stimulated mRNA expression and antimicrobial peptides in reconstructed epidermis. Interestingly, a predictive computer model that analyzed transducing events within skin epidermal cells suggested that this protective activity may involve the Toll-like receptor 2/protein kinase C, zeta transduction pathway. Protein kinase C, zeta inhibition was effectively shown to abolish VFB-induced gene stimulation and confirmed this hypothesis. This thus opens new avenues for investigation into the improvement of skin homeostatic defense in relation to the control of its physiological microbiota and innate immunity.

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

Mahé

Perez²,

Tacheau³

et al. 2013

CCID

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“…Aquaphilus dolomiae is a non-spore forming bacterium belonging to the Neisseriaceae family, which is isolated from Avène thermal Water (France), historically used in the management of chronic inflammatory skin diseases. Similarly, the lysate of Vitreoscilla filiformis (VF) has been shown to enhance skin defence mechanisms (102) and to decrease UV-induced sunburn cells in human skin, possibly by the activation of cutaneous regulatory T cells (103). VF is a filamentous Gram-negative aerobic bacterium belonging to the Neisseriaceae family found in LaRoche-Posay thermal water, historically applied to manage chronic inflammatory skin diseases (103–105).…”

Section: The Future Of Regenerative Surgerymentioning

confidence: 99%

Novel perspectives on the role of the human microbiota in regenerative medicine and surgery

et al. 2016

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Plastic surgery is transitioning from a fine craftsmanship to a regenerative science. In wound healing, the role of microorganisms is no longer considered to be just counteracting, but also promoting. Furthermore, host-microbe interactions are essential for numerous aspects of normal mammalian physiology, from metabolic activity to immune homeostasis. Each area of the human body hosts a unique microbial community, and the composition of microbiota is dependent on the host, age and the anatomical area, and it changes according to the characteristics of the microenvironment. Every squared centimeter of skin contains ~1 billion bacteria. The majority of microorganisms of the skin are commensal or temporary passing members. Skin flora mechanisms interacting or influencing the human physical skin barrier are not well defined. Resident skin bacteria provide the first line of defence against potentially dangerous pathogens and produce small molecules that influence their microbial neighbours. Furthermore, the microbiota activates and assists innate immunity and influences adaptive immunity. Various types of immune and non-immune cells contribute to wound healing. The proliferative phase of wound healing is inversely proportional to the extent of the post-traumatic inflammatory reaction. Topical bacterial lipopolysaccharide application markedly affects wound healing by accelerating the resolution of inflammation, increasing macrophage infiltration, enhancing collagen synthesis and altering the secretion of mediators involved in skin regeneration. Various studies have investigated the biological contents of thermal spring waters, and their anti-inflammatory and immune protective roles. In addition, the regenerative properties of thermal spring waters were analysed in an experimental animal wound model. The areas treated with thermal water healed faster than the areas treated with conventional dressings, and exhibited a collagen and elastic fiber network comparable with the normal skin. Thus, the microbial environment may be considered as a potential tool in regenerative medicine and surgery.

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“…These activities include: substance P antagonist (11), stimulation of skin anti-microbial peptide secretion, recovery of sunburn areas (12,13), activation of MnSOD as an inducible free-radical scavenger in keratinocytes for protecting cells against the uncontrolled production of reactive oxygen species (ROS) (14), modulation of mouse and human cutaneous inflammatory responses inducing interleukin (IL)-10 production in dendritic cells (DCs), and priming of regulatory T cells (15). It was shown that the activation of two independent TLR pathways occurs following DC stimulation with a V. filiformis lysate (13,15).…”

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confidence: 99%

Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies

Breton

Новиков

Martin

et al. 2017

Journal of Lipid Research

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bacterium. This is a signature for which each small detail may exert important differences on LPS recognition by host cells and signal transduction (2). LPS does not necessarily induce a toxic response, so all LPSs are not endotoxins, but endotoxins are always composed of LPSs. LPSs are the first nonsecreted toxins found to interact with host innate immunity, generating cytokine production, immune cell recruitment, and specific host-cell responses leading, at higher doses, to multiple organ failure and septic shock (3). The smallest change in the structures of LPSs or their hydrophobic moiety, named lipid A, is directly linked to innate immune recognition and development of a specific host response. The interaction between LPS molecules and their innate receptor, the MD-2/TLR4 complex, is greatly affected by the fine details of LPS structures. First, LPS molecules are recognized by the LPS binding protein and presented as monomers to the soluble or membrane CD14 receptors. Then, the LPS is transferred to the MD-2/TLR4 complex (2, 4) allowing its dimerization of the receptor through ionic and hydrophobic interactions and activation of the cellular signaling pathways. Among these, the activation of the NFB pathway directly induces rapid pro-inflammatory cytokine production. Secreted cytokines are ultimately central to the generation of efficient responses to pathogens and the activation of the adaptive immune system (5, 6).The Neisseriaceae family members, including the Vitreoscilla genus, are obligate aerobic bacteria described for the first time in 1949 by E. G. Pringsheim (7) as colorless gliding filamentous organisms. Similar organisms were in fact described earlier by Cohn (8) in 1870. They are known as the first genus in which bacterial hemoglobin (VHb) was discovered (9).Abstract Vitreoscilla filiformis is a Gram-negative bacterium isolated from spa waters and described for its beneficial effects on the skin. We characterized the detailed structure of its lipopolysaccharide (LPS) lipid A moiety, an active component of the bacterium that contributes to the observed skin activation properties. Two different batches differing in postculture cell recovery were tested. Chemical analyses and mass spectra, obtained before and after mild-alkali treatments, revealed that these lipids A share the common bisphosphorylated - (1→6) Lipopolysaccharides (LPSs) are the main components of the outer-membrane of Gram-negative bacteria, representing about 10 6 molecules per bacterium (1). They are also major antigens displaying unique structures for each

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Induction of the skin endogenous protective mitochondrial MnSOD byVitreoscilla filiformisextract

Cited by 20 publications

References 16 publications

A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

Novel perspectives on the role of the human microbiota in regenerative medicine and surgery

Structural and biological characteristics of different forms of V. filiformis lipid A: use of MS to highlight structural discrepancies

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