Induction Patterns of Chitinases in Yam Callus by Inoculation with Autoclaved Fusarium oxysporum, Ethylene, and Chitin and Chitosan Oligosaccharides

Differential Effect of Purified Spruce Chitinases and β-1,3-Glucanases on the Activity of Elicitors from Ectomycorrhizal Fungi

“…The finding that colloidal chitin could also induce chitinase in spruce cells allows us to believe that stimulated synthesis of chitinase might enhance the effectiveness of the chitin elicitorinactivating system. Such chitin-induced synthesis of chitinase was also demonstrated in Parfhenocissus quinquefolia cells, rice, and yam callus (Inui et al, 1991;Koga et al, 1992;Flach et al, 1993). Thus, inactivation of fungal chitinderived elicitors might be one of many instrumental preconditions required to create a compatible interaction of plant and fungus in the mycorrhiza.…”

Intracellular Localization of a Class IV Chitinase from Yam

“…Chitinase activity staining and -mannosidase activity assay. Chitinase activity on native PAGE was carried out by the method of Koga et al 20) In the case of the transformed tobacco suspension-cultured cells, the cultured cells and medium were separated by filtration with a filter paper/type 2 (Toyo Roshi Co., Ltd.) 7 days after subculture. The cells were homogenized in equal volumes of the extraction buffer and centrifuged at 4 C for 10 min at 15,000 rpm.…”

Induction of Fine Bent-grass Callus and Appearance of Chitinase during the Subculture

“…These observations suggest that the induction of chitinase is dependent on the species, tissue, and age of host plants, while plant callus, which is an aggregate of de-differentiated cells, seems to be suitable as a host with constant conditions. 5) In this study, we first induced calli from fine bent-grass (Agrostis alba L. var vulgaris THUNB), and established a subculture. The seeds were sterilized successively with 1 % Brij 35 for 0.5 min, 70% ethanol for 1 min, and 10% sodium hypochlorite solution for 1 hr, and then planted on Murashige-Skoog's agar (1 %) (MS) medium 6 ) containing 3% sucrose, and incubated at 27°C under a light.…”

“…Chitinase activity appeared all the time, but it increased a little around 25 days after the subculture. In order to investigate the pattern of chitinase isozyme during the subculture, activity staining for chitinase was done by the method of Koga et al 5 ) The above supernatant (l5ftl) of the homogenate was electrophoresed by denaturing (SDS--PAGE)S) and both acidic/neutraI 9 ) and basic non-denaturing polyacrylamide gel electrophoresis 10 ) (disc-PAGE), SDS·-and disc~PAGEs were done with 10 and 7.5% polyacrylamide slab gel, respectively. After electrophoresis, the proteins in the gel were transblotted into a 7.5% polyacrylamide gel containing 0.01 % glycol chitin.…”

“…Fine bent-grass callus seems to produce chitinase during the subculture without any treatments, unlike yam callus. 5) We are now investigating the induction of chitinase by treatment of this callus with some pathogens and elicitors.…”