Industrial Implementation of in Vitro Production of Monoclonal Antibodies

Dewar, Vincent; Voet, Pierre; Denamur, Françcoise; Smal, Jean

doi:10.1093/ilar.46.3.307

Cited by 25 publications

(13 citation statements)

References 6 publications

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“…Silk fibroin (SF) is a biocompatible, enzymatically degradable material, which can be processed into a bone‐like structure, for generation of bone 57, 58. The resorbable polymer scaffolds that have been investigated for the generation of cartilage include polylactic and polylactic‐glycolic acid,67–69 alginate,68, 70 silk,71, 72 polycaprolactone73, 74 and devitalized menisci 75…”

Section: Bioreactor Designsmentioning

confidence: 99%

“…The clinical use of hollow‐fibre modules for cell expansion was described almost 20 years ago by Knazek et al 104 Human tumour‐infiltrating lymphocytes isolated from patients with metastatic melanoma were expanded by 2–3 logs to produce around 10 10 lymphocytes. Since that time hollow‐fibre systems have been applied to mammalian cell culture processes including lymphocyte expansion,50, 105 gene transfer to hematopoietic cells,106 production of recombinant proteins and viruses,70, 107–111 hepatocyte culture and extracorporeal hepatic assist devices 74, 112–116. Hollow‐fibre bioreactors have been used to study cartilage development.…”

Section: Bioreactor Designsmentioning

confidence: 99%

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Amino Acid Ionic Liquid Modified Mesoporous Carbon: A Tailor‐made Nanostructure Biosensing Platform

Zhang

et al. 2012

ChemSusChem

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A novel nanocomposite based on ordered graphitized mesoporous carbon (GMC) and amino acid ionic liquids (AAIL) is obtained through controlled surface modification of GMC with hydrophilic AAILs (1-ethyl-3-methylimidazolium alanine, EMIM[Ala]), which is used as a platform for a tyrosinase biosensor to detect phenol. The GMC-AAIL nanocomposite possesses a better biocompatibility and improved aqueous-phase dispersion than hydrophobic GMC alone, owing to the introduction of hydrophilic and biocompatible AAILs. Comparative studies revealed that the catalytic activity of tyrosinase for phenol in phosphate buffer solution (PBS) containing EMIM[Ala] was about ten times higher than that in pure PBS. By entrapping tyrosinase molecules into the mesopores of GMC, making use of the synergy effect of GMC and AAIL (the "interspace confinement effect", the anti-fouling ability, and the biocompatible microenvironment), the GMC-AAIL-based biosensors display superior analytical performance to GMC-based ones in terms of signal-to-noise ratio, stability, repeatability, and working life. After 21-day storage, the electrode retained more than 90% of its initial response, indicating that surface modification of GMC with hydrophilic and biocompatible AAILs could significantly prolong the life of tyrosinase in vitro. The GMC10-EMIM[Ala]-based biosensor demonstrates a linear response for phenol concentrations from 0.1 to 10 µmol L(-1) with a low detection limit of 20 nmol L(-1) and sensitivity of 1385 mA cm(-2) M(-1). The GMC-AAIL nanocomposite proves to be a promising platform for enzyme-based biosensors and biocatalysis.

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Section: Bioreactor Designsmentioning

confidence: 99%

Section: Bioreactor Designsmentioning

confidence: 99%

Amino Acid Ionic Liquid Modified Mesoporous Carbon: A Tailor‐made Nanostructure Biosensing Platform

Zhang

et al. 2012

ChemSusChem

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“…The capacity of the cartridge can range from 1.5 ml to >1 l. There are several commercial sources for obtaining hollow fibre perfusion bioreactors (FiberCell Systems, Inc., Frederick, MD, USA; Spectrum Laboratories, Inc., Los Angeles, CA, USA; and Biovest International, Inc.). Recent reviews have critiqued the commercial hollow fibre perfusion bioreactor units available for the production of monoclonal antibodies [11,12].…”

Section: Antiviral Compounds For Viruses Grown In Cultured Cells Incmentioning

confidence: 99%

Antiviral Pharmacodynamics in Hollow Fibre Bioreactors

McSharry

Drusano

2011

Antivir Chem Chemother

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Pharmacodynamic investigation of antiviral compounds studies the relationship between drug exposure and the virological response. These studies are usually performed in animals and, eventually, in humans and are a very expensive proposition. To find a more efficient and less expensive method for determining pharmacodynamics of antiviral and antimicrobial compounds, the hollow fibre infection model (HFIM) system was developed to perform pharmacodynamic studies in vitro. This review covers the authors' studies on the use of in vitro hollow fibre bioreactor technologies for determining the pharmacodynamics of antiviral compounds for viruses grown in cultured cells, including HIV grown in CD4+ lymphoblastoid cells, vaccinia viruses grown in HeLa-S3 cells and influenza viruses grown in Madin-Darby canine kidney cells. Where possible, correlations between the pharmacodynamic index derived from the in vitro HFIM systems and clinical pharmacodynamic studies are made.

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“…For further information, we refer readers to discussions of in vitro systems and methods provided elsewhere in this issue (Dewar et al 2005;Lipman et al 2005). Also in this issue, Peterson (2005) discusses new approaches to MAb production.…”

Section: Immunization To Generate Antigen-specific B Cellsmentioning

confidence: 99%

Critical Steps in the Production of Polyclonal and Monoclonal Antibodies: Evaluation and Recommendations

Leenaars¹,

Hendriksen²

2005

ILAR Journal

173

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Antibodies are valuable tools in the laboratory and clinic. Antibodies include those secreted by a single clone of B lymphocytes, termed monoclonal antibodies, and those produced by a mixture of various B lymphocyte clones, termed polyclonal antibodies. Both products have become essential instruments in fundamental immunological research, immunohistochemistry, diagnostic testing, and vaccine quality control. Antibody production requires a substantial number of animals, and the animals are subjected to a number of invasive procedures such as antigen injection and blood collection. However, by carefully designing an immunization protocol and by optimizing the immunization response, it is possible to minimize animals' pain and distress while obtaining optimal immune responses. In this article, the critical steps in the production of polyclonal and monoclonal antibodies are described, specifically including selection of the animal species and its age, injection protocol, and ascites tapping. Recommendations are provided for optimizing the immunization response.

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Industrial Implementation of in Vitro Production of Monoclonal Antibodies

Cited by 25 publications

References 6 publications

Amino Acid Ionic Liquid Modified Mesoporous Carbon: A Tailor‐made Nanostructure Biosensing Platform

Amino Acid Ionic Liquid Modified Mesoporous Carbon: A Tailor‐made Nanostructure Biosensing Platform

Antiviral Pharmacodynamics in Hollow Fibre Bioreactors

Critical Steps in the Production of Polyclonal and Monoclonal Antibodies: Evaluation and Recommendations

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