Industrial Relevance of Trichoderma reesei as an Enzyme Producer

Fischer, Albert J.; Maiyuran, Suchindra; Yaver, Debbie

doi:10.1007/978-1-0716-1048-0_2

Cited by 9 publications

(10 citation statements)

References 54 publications

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“…Reesei . This fungus is notorious for its well-developed enzyme secretion system essential to its survival in the wild as a saprobe and which has been leveraged in numerous industrial processes including recombinant protein production . The strain used in this study, QM6a SecEYFP, has been modified to express yellow fluorescent protein (EYFP) under the same conditions of the main cellobiohydrolase, CBHI, and to follow the same intracellular path.…”

Section: Recent Applications To the Life Sciencesmentioning

confidence: 99%

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

V. D. dos Santos,

Hondl,

Ramos-Garcia

et al. 2023

ACS Meas. Sci. Au

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Despite the ubiquitous absorption of mid-infrared (IR) radiation by virtually all molecules that belong to the major biomolecules groups (proteins, lipids, carbohydrates, nucleic acids), the application of conventional IR microscopy to the life sciences remained somewhat limited, due to the restrictions on spatial resolution imposed by the diffraction limit (in the order of several micrometers). This issue is addressed by AFM-IR, a scanning probe-based technique that allows for chemical analysis at the nanoscale with resolutions down to 10 nm and thus has the potential to contribute to the investigation of nano and microscale biological processes. In this perspective, in addition to a concise description of the working principles and operating modes of AFM-IR, we present and evaluate the latest key applications of AFM-IR to the life sciences, summarizing what the technique has to offer to this field. Furthermore, we discuss the most relevant current limitations and point out potential future developments and areas for further application for fruitful interdisciplinary collaboration.

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Section: Recent Applications To the Life Sciencesmentioning

confidence: 99%

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

V. D. dos Santos,

Hondl,

Ramos-Garcia

et al. 2023

ACS Meas. Sci. Au

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“…Trichoderma reesei has been used for decades as an efficient microbial cell factory for the production of recombinant proteins, because of its excellent protein secretion ability (Fischer et al, 2021 ; Martinez et al, 2008 ). Meanwhile, T. reesei is superior in its capability to secrete cellulases and thus, the major fungus for industrial cellulase production (Bischof et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Enhancing the production of a heterologous Trametes laccase (LacA) by replacement of the major cellulase CBH1 in Trichoderma reesei

Zhang

et al. 2023

Journal of Industrial Microbiology and Biotechnology

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The laccases from white-rot fungi exhibit high redox potential in treating phenolic compounds. However, their application in commercial purposes has been limited because of the relatively low productivity by the native hosts. Here, the laccase A-encoding gene lacA of Trametes sp. AH28-2 was overexpressed under the control of the strong promoter of cbh1 (Pcbh1), the gene encoding the endogenous cellobiohydrolase 1 (CBH1), in the industrial workhorse fungus Trichoderma reesei. Firstly, the lacA expression cassette was randomly integrated into the T. reesei chromosome by genetic transformation. The lacA gene was successfully transcribed, but the laccase couldn't be detected in the liquid fermentation condition. Meanwhile, it was found that the endoplasmic reticulum associated degradation (ERAD) was strongly activated, indicating that expression of LacA probably triggered intense endoplasmic reticulum (ER) stress. Subsequently, the lacA expression cassette was added with the downstream region of cbh1 (Tcbh1) to construct the new expression cassette lacA::Δcbh1, which could replace the cbh1 locus in the genome via homologous recombination. After genetic transformation, the lacA gene was integrated into the cbh1 locus and transcribed. And the unfolded protein response (UPR) and ERAD were only slightly induced, for which the loss of endogenous cellulase CBH1 released the pressure of secretion. Finally, the maximum laccase activity of 168.3 U/L was obtained in the fermentation broth. These results demonstrated that the reduction of secretion pressure by deletion of endogenous protein-encoding genes would be an efficient strategy for secretion of heterologous target proteins in industrial fungi.

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“…The biodegradation of plant biomass represents a crucial step in the biorefinery industry and this process relies on the hydrolysis ability of lignocellulolytic enzymes to plant biomass substrates [ 2 – 4 ]. The filamentous fungus T. reesei strain QM6a was isolated from the Solomon Islands during the Second World War because of its destruction to army textiles, such as cotton-based tents and clothing [ 5 ]. As it turns out that T. reesei can secret various saccharification enzymes required for biomass degradation.…”

Section: Introductionmentioning

confidence: 99%

Current genetic strategies to investigate gene functions in Trichoderma reesei

Liu

Tang

et al. 2023

Microb Cell Fact

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The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina, Ascomycota) is a well-known lignocellulolytic enzymes-producing strain in industry. To increase the fermentation titer of lignocellulolytic enzymes, random mutagenesis and rational genetic engineering in T. reesei were carried out since it was initially found in the Solomon Islands during the Second World War. Especially the continuous exploration of the underlying regulatory network during (hemi)cellulase gene expression in the post-genome era provided various strategies to develop an efficient fungal cell factory for these enzymes’ production. Meanwhile, T. reesei emerges competitiveness potential as a filamentous fungal chassis to produce proteins from other species (e.g., human albumin and interferon α-2b, SARS-CoV-2 N antigen) in virtue of the excellent expression and secretion system acquired during the studies about (hemi)cellulase production. However, all the achievements in high yield of (hemi)cellulases are impossible to finish without high-efficiency genetic strategies to analyze the proper functions of those genes involved in (hemi)cellulase gene expression or secretion. Here, we in detail summarize the current strategies employed to investigate gene functions in T. reesei. These strategies are supposed to be beneficial for extending the potential of T. reesei in prospective strain engineering.

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Industrial Relevance of Trichoderma reesei as an Enzyme Producer

Cited by 9 publications

References 54 publications

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

Enhancing the production of a heterologous Trametes laccase (LacA) by replacement of the major cellulase CBH1 in Trichoderma reesei

Current genetic strategies to investigate gene functions in Trichoderma reesei

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