Inertial cell sorting of microparticle-laden flows: An innovative OpenFOAM-based arbitrary Lagrangian–Eulerian numerical approach

Abstract

The need for cell and particle sorting in human health care and biotechnology applications is undeniable. Inertial microfluidics has proven to be an effective cell and particle sorting technology in many of these applications. Still, only a limited understanding of the underlying physics of particle migration is currently available due to the complex inertial and impact forces arising from particle–particle and particle–wall interactions. Thus, even though it would likely enable significant advances in the field, very few studies have tried to simulate particle-laden flows in inertial microfluidic devices. To address this, this study proposes new codes (solved in OpenFOAM software) that capture all the salient inertial forces, including the four-way coupling between the conveying fluid and the suspended particles traveling a spiral microchannel. Additionally, these simulations are relatively (computationally) inexpensive since the arbitrary Lagrangian–Eulerian formulation allows the fluid elements to be much larger than the particles. In this study, simulations were conducted for two different spiral microchannel cross sections (e.g., rectangular and trapezoidal) for comparison against previously published experimental results. The results indicate good agreement with experiments in terms of (monodisperse) particle focusing positions, and the codes can readily be extended to simulate two different particle types. This new numerical approach is significant because it opens the door to rapid geometric and flow rate optimization in order to improve the efficiency and purity of cell and particle sorting in biotechnology applications.

A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability

“…The related boundary conditions include: (1) the channel wall is a rebound type, and (2) the initial velocity of the inlet fluid is based on the velocity field in the medium. According to Newton’s second law, the balance of many forces determines the trajectory of a cell in a DEP-based microfluidic system with a laminar flow, which is described as follows 23 , 24 : …”