Inertial focusing with sub-micron resolution for separation of bacteria

Cruz, Javier; Graells, Tiscar; Walldén, Mats; Hjort, Klas

doi:10.1039/c9lc00080a

Cited by 56 publications

(49 citation statements)

References 41 publications

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“…In another study 43 A threshold Reynold's number was found for each particle size, at which, microparticles will be trapped inside a laminar microvortex. As the desired particle size decreases, the forces affecting it decreases drastically as Cruz et al 44 described when aiming to focus particles as small as 1 mm. Therefore, when aiming to focus smaller particles (less than 5 mm), this force reduction has to be compensated by increasing the ow rate or by decreasing the channel dimensions.…”

Section: Introductionmentioning

confidence: 99%

Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

et al. 2019

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“…With a laser-induced fluorescence module integrated, the on-chip flow cytometer system could offer a high detection throughput of 2100 particles/s. Moreover, using the adjusted curved spiral microchannel, Cruz et al realized ultrasensitive submicro particle focusing [48]. Particles with size between 0.5 µm to 2.0 µm could be aligned on corresponding equilibrium positions at the end of the microchannel.…”

Section: Spiral Microchannelmentioning

confidence: 99%

A Review of Secondary Flow in Inertial Microfluidics

et al. 2020

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Inertial microfluidic technology, which can manipulate the target particle entirely relying on the microchannel characteristic geometry and intrinsic hydrodynamic effect, has attracted great attention due to its fascinating advantages of high throughput, simplicity, high resolution and low cost. As a passive microfluidic technology, inertial microfluidics can precisely focus, separate, mix or trap target particles in a continuous and high-flow-speed manner without any extra external force field. Therefore, it is promising and has great potential for a wide range of industrial, biomedical and clinical applications. In the regime of inertial microfluidics, particle migration due to inertial effects forms multiple equilibrium positions in straight channels. However, this is not promising for particle detection and separation. Secondary flow, which is a relatively minor flow perpendicular to the primary flow, may reduce the number of equilibrium positions as well as modify the location of particles focusing within channel cross sections by applying an additional hydrodynamic drag. For secondary flow, the pattern and magnitude can be controlled by the well-designed channel structure, such as curvature or disturbance obstacle. The magnitude and form of generated secondary flow are greatly dependent on the disturbing microstructure. Therefore, many inventive and delicate applications of secondary flow in inertial microfluidics have been reported. In this review, we comprehensively summarize the usage of the secondary flow in inertial microfluidics.

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“…(1) Main Straight Channel for Inertial Migration Effect The main straight channel with rectangular cross sections is located in the entrance, and connects the curve channels. As Poiseuille flow naturally presents in the microchannels, particles/cells usually are acted upon by two fluid dynamic effects-wall-induced lift forces and shear-gradient lift forces-for their discrete equilibrium positions [33,34]. The wall-induced lift forces push the particles/cells away from the walls, while the shear-gradient lift forces push the particles/cells towards the walls [35].…”

Section: Device Design and Working Principlementioning

confidence: 99%

High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces

et al. 2020

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A microfluidic chip, which can separate and enrich leukocytes from whole blood, is proposed. The chip has 10 switchback curve channels, which are connected by straight channels. The straight channels are designed to permit the inertial migration effect and to concentrate the blood cells, while the curve channels allow the Dean flow to further classify the blood cells based on the cell sizes. Hydrodynamic suction is also utilized to remove smaller blood cells (e.g., red blood cell (RBC)) in the curve channels for higher separation purity. By employing the inertial migration, Dean flow force, and hydrodynamic suction in a continuous flow system, our chip successfully separates large white blood cells (WBCs) from the whole blood with the processing rates as high as 1 × 108 cells/sec at a high recovery rate at 93.2% and very few RBCs (~0.1%).

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Inertial focusing with sub-micron resolution for separation of bacteria

Abstract: Inertial focusing in curved channels is demonstrated for particles between 0.5 and 2.0 μm in diameter; a range of biological relevance since it comprises a multitude of bacteria and organelles of eukaryotic cells.

Cited by 56 publications

References 41 publications

Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

A Review of Secondary Flow in Inertial Microfluidics

High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces

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