Infantile D and V

A “Smart” Magnetic Resonance Imaging Agent That Reports on Specific Enzymatic Activity

“…4); a total of 5603 reflections measured, 2981 used for refinemen t . Fluorescence experiments [5,6] with EGad: The decay rate (inversely proportional to the lifetime) of the emission peak at 1 . In three identical inversion-recovery (IR-NMR) high resolution experiments (Bruker AMX 500, 26°C) EGad(2 mM) was incubated with two different concentrations ofp-gal (1.7 p~ and 5.1 PM) heat-inactivated p-gal(l0 min at 80', 5.1 pM), and EGad alone (2 mM) in phosphate buffer (25 mM, pH 7.3) at 37 "C. Minimal enzyme concentrations were used to reduce potential interactions between the contrast agent and the enzyme.…”