Infection Status of Freshwater Fish with Metacercariae of Clonorchis sinensis in Korea

Abstract

This study investigated freshwater fish for their current infection status with metacercariae of Clonorchis sinensis in Korea. Twenty-one species of freshwater fish (n = 677) were collected from 34 regions nationwidely from February 2007 to June 2008. They were individually examined by digestion technique. Eight species of freshwater fish from 17 different regions were recognized positive for the metacercariae of C. sinensis. The positive rates (range of metacercariae number per fish) of fish by the species were as follows: 48% (1-1,142) in Pseudorasbora parva, 60% (1-412) in Pungtungia herzi, 15.7% (1-23) in Pseudogobio esocinus, 29% (1-7) in Acheilognathus intermedia, 21% (1-4) in Odontobutis interrupta, 33% (1-6) in Zacco temmincki, 3.6% (1-4) in Zacco platypus, and 26.3% (1) in Hemibarbus labeo. The two species, P. parva and P. herzi, are able to be the index fish for estimation of C. sinensis transmission in a certain locality. Still several species of freshwater fish are briskly transmitting C. sinensis infection in many riverside areas of southern Korea.

Prevalence ofClonorchis sinensisMetacercariae in Freshwater Fish from Three Latitudinal Regions of the Korean Peninsula

“…In the study by Kim et al [12], all the fish from upper regions of Cheongju, Chungcheongbuk-do were negative for Cs Mc, even though the number of susceptible fishes examined were limited. It is interesting that Cs Mc were detected only in fishes from Hantan-gang in northern areas.…”

Trematode Metacercariae in Freshwater Fish from Water Systems of Hantangang and Imjingang in Republic of Korea

“…On the other hand, the infection status of FZT metacercariae in the second intermediate hosts is used as an important epidemiological index together with those of the infection status with adult worms in the definitive and/or reservoir hosts. Thus, many Korean workers have investigated the infection status of fish from various endemic areas with FZT metacercariae to estimate the endemicities of FZT infections [ 3 - 10 , 14 - 21 ]. Most of these studies were performed on specific trematode species, such as Clonorchis sinensis , Metagonimus spp.…”

Proteomic analysis of differentially expressed proteins in human cholangiocarcinoma cells treated withClonorchis sinensisexcretory–secretory products

“…5), as ESP of C. sinensis and O. viverrini are potential promoters for the development of cholangiocarcinoma [Thuwajit et al, 2004;Kim et al, 2008] and Trx 1 and Prdx 6 expression patterns are significantly associated with tumor progression. Trx 1 is an oxidoreductase containing two redox-active cysteine residues within a conserved active center, which can be oxidized to form intramolecular disulfide bonds.…”

“…The former is caused by direct contact of parasites with bile duct epithelium, and the latter by their ESP [Vatanasapt et al, 1999]. In particular, ESP from liver flukes is potent inducers of various pathophysiologic responses in host cells [Cervi and Masih, 1997;Thuwajit et al, 2004;Serradell et al, 2007;Kim et al, 2008;Pak et al, 2009]. Therefore, we performed experiments using a cell culture system (human cholangiocarcinoma cell line, HuCCT1) with C. sinensis ESP to analyze the expression patterns of host cellular proteins.…”

“…Cells exposed to liver fluke ESP display diverse physiologic responses, depending on the cell type. For example, ESP from C. sinensis and Opisthorchis viverrini stimulate proliferation in HEK293 and NIH/3T3 cells via induction of E2F1 transcription factor and cell cycle regulatory proteins, respectively [Thuwajit et al, 2004;Kim et al, 2008]. On the other hand, treatment with F. hepatica ESP inhibits proliferative responses to mitogens in rat splenocytes [Cervi and Masih, 1997] and causes apoptotic cell death in rat eosinophils via activation of tyrosine kinases and the caspase cascade [Serradell et al, 2007].…”