Infection with 2009 H1N1 Influenza Virus Primes for Immunological Memory in Human Nose-Associated Lymphoid Tissue, Offering Cross-Reactive Immunity to H1N1 and Avian H5N1 Viruses

Mahallawi, Waleed H.; Kasbekar, Anand V.; McCormick, M; Höschler, Katja; Temperton, Nigel; Leong, Samuel; Beer, Helen; Ferrara, Francesca; McNamara, Paul; Zhang, Qibo

doi:10.1128/jvi.03547-12

Cited by 27 publications

(29 citation statements)

References 46 publications

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“…Production of anti-HA IgG, IgM, and IgA antibodies to sH1N1 virus in cell culture supernatants was measured as previously described ( 44 , 45 ). In brief, ELISA plates were coated with recombinant sH1N1 HA overnight.…”

Section: Methodsmentioning

confidence: 99%

Activation and Induction of Antigen-Specific T Follicular Helper Cells Play a Critical Role in Live-Attenuated Influenza Vaccine-Induced Human Mucosal Anti-influenza Antibody Response

Al-jurayyan

Puksuriwong

Ahmed

et al. 2018

J Virol

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There is increasing interest recently in developing intranasal vaccines against respiratory tract infections. The antibody response is critical for vaccine-induced protection, and T follicular helper cells (TFH) are considered important for mediating the antibody response. Most data supporting the role for TFH in the antibody response are from animal studies, and direct evidence from humans is limited, apart from the presence of TFH-like cells in blood. We studied the activation and induction of TFH and their role in the anti-influenza antibody response induced by a live-attenuated influenza vaccine (LAIV) in human nasopharynx-associated lymphoid tissue (NALT). TFH activation in adenotonsillar tissues was analyzed by flow cytometry, and anti-hemagglutinin (anti-HA) antibodies were examined following LAIV stimulation of tonsillar mononuclear cells (MNC). Induction of antigen-specific TFH by LAIV was studied by flow cytometry analysis of induced TFH and CD154 expression. LAIV induced TFH proliferation, which correlated with anti-HA antibody production, and TFH were shown to be critical for the antibody response. Induction of TFH from naive T cells by LAIV was shown in newly induced TFH expressing BCL6 and CD21, followed by the detection of anti-HA antibodies. Antigen specificity of LAIV-induced TFH was demonstrated by expression of the antigen-specific T cell activation marker CD154 upon challenge by H1N1 virus antigen or HA. LAIV-induced TFH differentiation was inhibited by BCL6, interleukin-21 (IL-21), ICOS, and CD40 signaling blocking, and that diminished anti-HA antibody production. In conclusion, we demonstrated the induction by LAIV of antigen-specific TFH in human NALT that provide critical support for the anti-influenza antibody response. Promoting antigen-specific TFH in NALT by use of intranasal vaccines may provide an effective vaccination strategy against respiratory infections in humans.IMPORTANCE Airway infections, such as influenza, are common in humans. Intranasal vaccination has been considered a biologically relevant and effective way of immunization against airway infection. The vaccine-induced antibody response is crucial for protection against infection. Recent data from animal studies suggest that one type of T cells, TFH, are important for the antibody response. However, data on whether TFH-mediated help for antibody production operates in humans are limited due to the lack of access to human immune tissue containing TFH. In this study, we demonstrate the induction of TFH in human immune tissue, providing critical support for the anti-influenza antibody response, by use of an intranasal influenza vaccine. Our findings provide direct evidence that TFH play a critical role in vaccine-induced immunity in humans and suggest a novel strategy for promoting such cells by use of intranasal vaccines against respiratory infections.

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Section: Methodsmentioning

confidence: 99%

Activation and Induction of Antigen-Specific T Follicular Helper Cells Play a Critical Role in Live-Attenuated Influenza Vaccine-Induced Human Mucosal Anti-influenza Antibody Response

Al-jurayyan

Puksuriwong

Ahmed

et al. 2018

J Virol

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“…Recent data suggest that broadly reactive anti-HA mAbs may require FcγR engagement for protection regardless of neutralization or HA domain specificity (25). Influenza vaccination and infection studies with pandemic and seasonal H1N1 viruses have been shown to recall memory B cell responses that cross-react with avian H5N1 viruses (47)(48)(49). While the breadth of anti-HA Abs has been thoroughly examined for group 1 viruses, it had not been previously studied during severe H7N9 and seasonal influenza in humans.…”

Section: Discussionmentioning

confidence: 99%

Fc functional antibodies in humans with severe H7N9 and seasonal influenza

Vanderven¹,

Liu²,

Ana-Sosa-Batiz³

et al. 2017

JCI Insight

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“…Adjuvanted inactivated whole virion and split virion H5N1 vaccines elicited neutralizing antibodies against homologous virus and drifted H5N1 viruses representing distinct clades in children, adults and the elderly [85][86][87][88]. Furthermore, H1N1pdm vaccine elicited H5N1-reactive memory B-cell responses in the upper respiratory mucosa [89].…”

Section: Pandemic Influenza Vaccines Can Elicit Cross-protective Antimentioning

confidence: 99%

Refining the Approach to Vaccines Against Influenza a Viruses with Pandemic Potential

Czakó

Subbarao

2015

Future Virol.

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Vaccination is the most effective strategy for prevention and control of influenza. Timely production and deployment of seasonal influenza vaccines is based on an understanding of the epidemiology of influenza and on global disease and virologic surveillance. Experience with seasonal influenza vaccines guided the initial development of pandemic influenza vaccines. A large investment in pandemic influenza vaccines in the last decade has resulted in much progress and a body of information that can now be applied to refine the established paradigm. Critical and complementary considerations for pandemic influenza vaccines include improved assessment of the pandemic potential of animal influenza viruses, proactive development and deployment of pandemic influenza vaccines, and application of novel platforms and strategies for vaccine production and administration.

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Infection with 2009 H1N1 Influenza Virus Primes for Immunological Memory in Human Nose-Associated Lymphoid Tissue, Offering Cross-Reactive Immunity to H1N1 and Avian H5N1 Viruses

Cited by 27 publications

References 46 publications

Activation and Induction of Antigen-Specific T Follicular Helper Cells Play a Critical Role in Live-Attenuated Influenza Vaccine-Induced Human Mucosal Anti-influenza Antibody Response

Activation and Induction of Antigen-Specific T Follicular Helper Cells Play a Critical Role in Live-Attenuated Influenza Vaccine-Induced Human Mucosal Anti-influenza Antibody Response

Fc functional antibodies in humans with severe H7N9 and seasonal influenza

Refining the Approach to Vaccines Against Influenza a Viruses with Pandemic Potential

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