Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris

Niu, Jinzhi; Meeus, Ivan; Coninck, Dieter Im De; Deforce, Dieter; Etebari, Kayvan; Asgari, Sassan; Smagghe, Guy

doi:10.1038/srep45620

Cited by 9 publications

(8 citation statements)

References 41 publications

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“…[ 47 ]. All viruses from the AKI-complex replicate in Bombus [ 46 , 54 , 105 , 106 ], and negative replicative forms were also detected in Vespa velutina [ 68 , 107 ], as well as bees from the Halictidae family [ 46 ] and several ant species [ 58 , 59 ]. Finally, BQCV has been shown to replicate in Bombus spp.…”

Section: Discussionmentioning

confidence: 99%

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Dalmon

Diévart

Thomasson

et al. 2021

Insects

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Viruses are known to contribute to bee population decline. Possible spillover is suspected from the co-occurrence of viruses in wild bees and honey bees. In order to study the risk of virus transmission between wild and managed bee species sharing the same floral resource, we tried to maximize the possible cross-infections using Phacelia tanacetifolia, which is highly attractive to honey bees and a broad range of wild bee species. Virus prevalence was compared over two years in Southern France. A total of 1137 wild bees from 29 wild bee species (based on COI barcoding) and 920 honey bees (Apis mellifera) were checked for the seven most common honey bee RNA viruses. Halictid bees were the most abundant. Co-infections were frequent, and Sacbrood virus (SBV), Black queen cell virus (BQCV), Acute bee paralysis virus (ABPV) and Israeli acute paralysis virus (IAPV) were widespread in the hymenopteran pollinator community. Conversely, Deformed wing virus (DWV) was detected at low levels in wild bees, whereas it was highly prevalent in honey bees (78.3% of the samples). Both wild bee and honey bee virus isolates were sequenced to look for possible host-specificity or geographical structuring. ABPV phylogeny suggested a specific cluster for Eucera bees, while isolates of DWV from bumble bees (Bombus spp.) clustered together with honey bee isolates, suggesting a possible spillover.

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Section: Discussionmentioning

confidence: 99%

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Dalmon

Diévart

Thomasson

et al. 2021

Insects

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“…Using RNA interference (RNAi) to suppress the expression levels of candidate genes is a powerful method for inferring gene function (Kanehisa and Goto, 2000;Scherr et al, 2003). When the candidate gene is a critical part of the RNAi pathway itself, such as Dicer, it can be extremely challenging to use siRNA to test for function (Niu et al, 2017). When the expression level of Dicer is too low, the efficiency of the RNAi pathway can be reduced (Niu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Dicer regulates Nosema ceranae proliferation in honeybees

Huang

Chen

et al. 2018

Insect Molecular Biology

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Nosema ceranae is a microsporidian parasite that infects the honeybee midgut epithelium. The protein-coding gene Dicer is lost in most microsporidian genomes but is present in N. ceranae. By feeding infected honeybees with small interfering RNA targeting the N. ceranae gene coding Dicer (siRNA-Dicer), we found that N. ceranae spore loads were significantly reduced. In addition, over 10% of total parasite protein-coding genes showed significantly divergent expression profiles after siRNA-Dicer treatment. Parasite genes for cell proliferation, ABC transporters and hexokinase were downregulated at 3 days postinfection, a key point in the middle of parasite replication cycles. In addition, genes involved in metabolic pathways of honeybees and N. ceranae showed significant co-expression. Furthermore, the siRNA-Dicer treatment partly reversed the expression patterns of honeybee genes. The honeybee gene mucin-2-like showed significantly upregulation in the siRNA-Dicer group compared with the infection group continually at 4, 5 and 6 days postinfection, suggesting that the siRNA-Dicer feeding promoted the strength of the mucus barrier resulted from interrupted parasite proliferation. As the gene Dicer broadly regulates N. ceranae proliferation and honeybee metabolism, our data suggest the RNA interference pathway is an important infection strategy for N. ceranae.

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“…Although antiviral immunity in insects has been a focus of attention in the past years, studies have focused on few species (flies, vector mosquitoes, honeybees) and specific viruses (e.g. [28][29][30][31]). Broader investigations have been hampered by lack of information on the viruses infecting insects, which could be used to trigger antiviral immunity.…”

Section: Evolutionary Immunology To Delve Into the Biodiversity Of An...mentioning

confidence: 99%

Evolutionary immunology to explore original antiviral strategies

Imler,

Cai,

Meignin

et al. 2024

Phil. Trans. R. Soc. B

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Over the past 25 years, the field of evolutionary developmental biology (evo–devo) has used genomics and genetics to gain insight on the developmental mechanisms underlying the evolution of morphological diversity of animals. Evo–devo exploits the key insight that conserved toolkits of development (e.g. Hox genes) are used in animals to produce genetic novelties that provide adaptation to a new environment. Like development, immunity is forged by interactions with the environment, namely the microbial world. Yet, when it comes to the study of immune defence mechanisms in invertebrates, interest primarily focuses on evolutionarily conserved molecules also present in humans. Here, focusing on antiviral immunity, we argue that immune genes not conserved in humans represent an unexplored resource for the discovery of new antiviral strategies. We review recent findings on the cGAS-STING pathway and explain how cyclic dinucleotides produced by cGAS-like receptors may be used to investigate the portfolio of antiviral genes in a broad range of species. This will set the stage for evo–immuno approaches, exploiting the investment in antiviral defences made by metazoans over hundreds of millions of years of evolution. This article is part of the theme issue ‘Sculpting the microbiome: how host factors determine and respond to microbial colonization’.

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Infections of virulent and avirulent viruses differentially influenced the expression of dicer-1, ago-1, and microRNAs in Bombus terrestris

Cited by 9 publications

References 41 publications

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Dicer regulates Nosema ceranae proliferation in honeybees

Evolutionary immunology to explore original antiviral strategies

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