Infective Endocarditis Caused by Pseudomonas stutzeri: A Case Report and Literature Review

Alwazzeh, Marwan J; Alkuwaiti, Feras A.; Alqasim, Moammer; Alwarthan, Sarah; Elghoneimy, Yasser

doi:10.3390/idr12030020

Cited by 15 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, the aforementioned results should be considered clinically significant because variations in the oropharyngeal microbiota of COVID-19 patients can be used as rapid and non-invasive bacterial biomarkers of dysbiosis of the gut microbiota or invasion of potential pathogens in the gut region that cannot be reflected by feces. Campylobacter and P. stutzeri , two enriched taxa in the oral cavity of COVID-19 patients, are reported as opportunistic pathogens for periodontitis progression, pneumonia, meningitis, and infective endocarditis ( Ihara et al., 2003 ; Tasdelen Fisgin et al., 2004 ; Alwazzeh et al., 2020 ). Though the enrichment of Campylobacter in LCP patients was in agreement with the previous publication ( Wu et al., 2021 ), that the majority of oral bacteria increased or decreased in COVID-19 patients in our work was shown to disagree with other reports.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Altered Oropharyngeal Microbiota in Hospitalized Patients With Mild SARS-CoV-2 Infection

Shi

Han

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Coronavirus disease 2019 (COVID-19) remains a serious emerging global health problem, and little is known about the role of oropharynx commensal microbes in infection susceptibility and severity. Here, we present the oropharyngeal microbiota characteristics identified by full-length 16S rRNA gene sequencing through the NANOPORE platform of oropharynx swab specimens from 10 mild COVID-19 patients and 10 healthy controls. Our results revealed a distinct oropharyngeal microbiota composition in mild COVID-19 patients, characterized by enrichment of opportunistic pathogens such as Peptostreptococcus anaerobius and Pseudomonas stutzeri and depletion of Sphingomonas yabuuchiae, Agrobacterium sullae, and Pseudomonas veronii. Based on the relative abundance of the oropharyngeal microbiota at the species level, we built a microbial classifier to distinguish COVID-19 patients from healthy controls, in which P. veronii, Pseudomonas fragi, and S. yabuuchiae were identified as the most prominent signatures for their depletion in the COVID-19 group. Several members of the genus Campylobacter, especially Campylobacter fetus and Campylobacter rectus, which were highly enriched in COVID-19 patients with higher severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral load and showed a significant correlation with disease status and several routine clinical blood indicators, indicate that several bacteria may transform into opportunistic pathogen in COVID-19 patients when facing the challenges of viral infection. We also found the diver taxa Streptococcus anginosus and Streptococcus alactolyticus in the network of disease patients, suggesting that these oropharynx microbiota alterations may impact COVID-19 severity by influencing the microbial association patterns. In conclusion, the low sample size of SARS-CoV-2 infection patients (n = 10) here makes these results tentative; however, we have provided the overall characterization that oropharyngeal microbiota alterations and microbial correlation patterns were associated with COVID-19 severity in Anhui Province.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of Altered Oropharyngeal Microbiota in Hospitalized Patients With Mild SARS-CoV-2 Infection

Shi

Han

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The source of infection could not be identified in any of the cases. All patients were successfully treated with surgery and antibiotics and survived, except one patient [ 8 , 11 – 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…[ 13 ] 2018 Lebanon Prosthetic aortic Ceftazidime Aortic and tricuspid valves replacement Deceased 26 days Alwazzeh et al. [ 14 ] 2020 Saudi Arabia Native mitral and aortic Vancomycin, ceftriaxone, and cefepime Mitral and aortic valves replacement Cured Not applicable …”

Section: Discussionmentioning

confidence: 99%

A relapsed Pseudomonas stutzeri prosthetic valve endocarditis: a case report and review of the literature

et al. 2021

View full text Add to dashboard Cite

Background Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. It is a Gram-negative bacterium and a common inhabitant of soil and water. Case presentation We report the case of a 51-year-old arab gentleman who has systemic lupus erythematous complicated by lupus nephritis and underwent renal transplantation twice. He underwent mitral valve replacement and 4 years later was diagnosed with prosthetic valve endocarditis caused by Pseudomonas stutzeri. Conclusions Literature review was conducted and revealed that this pathogen may be of a particular medical relevance in immunocompromised patients. Our case proves that early infection and relapse despite optimal antibiotics course are possible outcomes of Pseudomonas stutzeri endocarditis. To the best of our knowledge, this is the second case of fulminant early prosthetic valve endocarditis occurring only 1 month post-cardiac surgery with relapse despite a complete antibiotics course.

show abstract

“…Pseudomonas stutzeri is ubiquitous and rarely cause infections (Lalucat et al, 2006;Park et al, 2013) . This bacteria has been reported as an oportunistic pathogen, which cause some infection, such as hospital-acquired infection (HAI) (Noble and Overman, 1994), septicemia (Bello, 2007), peritonitis (Park et al, 2013), endocarditis (Alwazzeh et al, 2020) and urinary tract (Taneja et al, 2004). Based on the result, it could be concluded that no Leptospira was found in urine samples of sanitary worker.…”

Section: Isolation and Molecular Identificationmentioning

confidence: 99%

Study of Risk Factors and Leptospira Detection of Sanitary Workers in Jakarta, Indonesia

et al. 2022

View full text Add to dashboard Cite

Leptospirosis is zoonotic disease, caused by spirochete-bacteria Leptospira, transmitted by excreted urine of rodent into the environment. Sanitary workers had high-risk of Leptospira infection due to frequent exposure to contaminated environment, which could cause asymptomatic leptospiruria (the presence of leptospira in urine) and severe complications, such as chronic kidney disease. The aim of this study was to find leptospiruria in sanitary workers, using PCR method, targeting specific genes of Leptospira. Urine samples and questionnaires were obtained from fifteen sanitary workers. Samples were cultured in EMJH-broth with addition of 5-fluorouracil, incubated for 3 months and observed for growth of bacteria using dark-field microscope. Identification of bacteria was performed by PCR, targeting lipl32, rrl, flaB, ompL1 genes, followed by sequencing using Sanger method, alignment using ClustalW and BLAST. The questionnaires result showed that 26,7% of respondent had medium level of risk factors, and 53,3% of respondent had applied good prevention for leptospirosis. Pearson correlation analysis showed that there was relationship between risk factors and prevention. Culture result showed growth in four samples, and analysis by PCR only showed rrl-PCR had expected amplicon. However sequencing result showed that the amplicon had 99% homogeneity to Pseudomonas stutzeri. In conclusion, Leptospira was not found in the urine of sanitary worker, might be due to applied good prevention for leptospirosis.Keywords: Leptospira, Leptospiruria, Sanitary Worker, Risk Factors ABSTRAK Leptospirosis adalah penyakit zoonosis, yang disebabkan oleh bacteria Leptospira Sp., ditularkan melalui urin hewan pengerat yang dikeluarkan ke lingkungan. Pekerja sanitasi memiliki risiko tinggi terinfeksi Leptospira karena sering terpapar lingkungan yang terkontaminasi, yang dapat menyebabkan leptospiruria (adanya liptospira dalam urin) tanpa gejala, dan komplikasi berat, seperti penyakit ginjal kronis. Tujuan dari penelitian ini adalah untuk menemukan leptospiruria pada petugas kebersihan, menggunakan metode PCR, menargetkan gen spesifik Leptospira. Sampel urin dan kuesioner diperoleh dari lima belas petugas kebersihan. Sampel dikultur dalam EMJH-broth dengan penambahan 5-fluorouracil, diinkubasi selama 3 bulan dan diamati pertumbuhan bakterinya menggunakan mikroskop medan gelap. Identifikasi bakteri dilakukan dengan PCR, menargetkan gen lipl32, rrl, flaB, ompL1, dilanjutkan dengan sequencing menggunakan metode Sanger, alignment menggunakan ClustalW dan BLAST. Hasil kuesioner menunjukkan bahwa 26,7% responden memiliki faktor risiko tingkat sedang, dan 53,3% responden telah menerapkan pencegahan leptospirosis yang baik. Analisis korelasi Pearson menunjukkan bahwa ada hubungan antara faktor risiko dan pencegahan. Hasil kultur menunjukkan pertumbuhan pada keempat sampel, dan analisis dengan PCR hanya menunjukkan rrl-PCR memiliki amplikon yang diharapkan. Namun hasil sekuensing menunjukkan bahwa amplikon memiliki homogenitas 99% terhadap Pseudomonas stutzeri. Kesimpulannya, Leptospira tidak ditemukan dalam urin petugas kebersihan, mungkin karena penerapan pencegahan leptospirosis yang baik.Kata kunci: Leptospira, Leptospiruria, Petugas Kebersihan, Faktor Risiko

show abstract

Infective Endocarditis Caused by Pseudomonas stutzeri: A Case Report and Literature Review

Cited by 15 publications

References 14 publications

Characterization of Altered Oropharyngeal Microbiota in Hospitalized Patients With Mild SARS-CoV-2 Infection

Characterization of Altered Oropharyngeal Microbiota in Hospitalized Patients With Mild SARS-CoV-2 Infection

A relapsed Pseudomonas stutzeri prosthetic valve endocarditis: a case report and review of the literature

Study of Risk Factors and Leptospira Detection of Sanitary Workers in Jakarta, Indonesia

Contact Info

Product

Resources

About