Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

Zheng, Haixue; Liu, Xiangtao; Shang, Youjun; Wu, Jiang; Bai, Xingwen; Jin, Ye; Sun, Shiqi; Guo, Huichen; Tian, Hong; Feng, Xia; Yin, Shuanghui; Guo, Jianpeng; Cong, Guozheng; Liu, Zaixin; Chang, Huiyun; Ma, Jingjiao; Xie, Qiang

doi:10.1007/s11434-006-2095-z

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Since then, several infectious clones of picornaviruses have been constructed. These include Hepatovirus [39] , Enterovirus [40,41] , Rhinoviruses [42] and Cardioviruses [30,43,44] , and the first infectious clone of FMDV (Aphthovirus) O1K strain was recovered by Zibert et al [16] . In 1993/2004, infectious clones of FMDV A12 and ZIM/7/83 (SATⅡ serotype) were constructed by Rieder et al [15] and van Rensburg et al [17] , respectively.…”

Section: Discussionmentioning

confidence: 99%

Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain

Bai

Cao

et al. 2009

SCI CHINA SER C

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Two full-length genomic cDNA clones, pTA/FMDV and pCA/FMDV, were constructed that contained three point-mutants [A174G and A308G (not present in pTA/FMDV); T1029G] in the genome compared with the wild type A/AKT/58 strain of foot-and-mouth disease virus. These two viruses were rescued by co-transfection of pCA/FMDV with pCT7RNAP, which can express T7 RNA polymerase in BHK-21 cell-lines, or by transfection of the in vitro transcribed RNA. Their biological properties were analyzed for their antigenicity, virulence in suckling-mice (LD50) and growth kinetics in BHK-21 cells. The in vivo rescued viruses showed high pathogenicity for 3-day-old unweaned mice (LD50=10(-7.5)). However, the in vitro transcribed RNA derived from pTA/FMDV had lower pathogenicity for suckling-mice (LD50=10(-6)), and the in vivo transcribed RNA recovered from pCA/FMDV co-transfected with pCT7RNAP showed no significant differences from the wild type virus. These data showed that recovery of the infectious foot-and-mouth disease virus directly from the use of in vivo techniques was better than from in vitro methods. Furthermore, the reverse genetic procedure technique was simplified to a faster one-step procedure based on co-transfection with pCT7RNAP. These results suggest that in vivo RNA transcripts may be more valuable for engineering recombinant foot-and-mouth disease virus than in vitro RNA transcripts, and may contribute to further understanding of the biological properties, such as replication, maturation and quasispecies, of the foot-and-mouth disease virus.

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Section: Discussionmentioning

confidence: 99%

Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain

Bai

Cao

et al. 2009

SCI CHINA SER C

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“…The harvested silkworm haemolymph was centrifuged and the supernatant was examined to observe viral empty capsids. Samples were observed in an EM at 80000× magnification according to the test protocol [15] .…”

Section: Observation Of Viral Empty Capsidsmentioning

confidence: 99%

Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae

Yao

Yin

et al. 2007

CHINESE SCI BULL

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The intact open reading frame (ORF) of foot-and-mouth disease virus (FMDV) Asia I/XJ strain was amplified by RT-PCR and inserted into the transfer vector pVL1393 to generate plasmid pVL-ORF. Bm-N cells were transfected with pVL-ORF and linearized Bm-BacPAK6 DNA, and the recombinant silkworm baculovirus Bm-ORF containing the full ORF of FMDV was obtained. The results of indirect immunofluorescence assay (IFA) showed that Bm-ORF could be expressed efficiently in Bm-N cell. After inoculating the early 5th instar larvae of silkworm, the polyprotein of FMDV could be detected by sandwich ELISA and empty capsid-like particles could be observed under the electron microscope. Expression products from silkworm were used as the antigen to immunize the cattle. The specific antibody was induced in all vaccinated animals. The immunized cattle were challenged with the virulent FMDV Asia I/XJ strain, two of the four cattle were completely protected and clinical symptoms were alleviated and delayed in the others. The results suggest that this strategy might be used to develop the new subunit FMDV vaccine.foot-and-mouth disease virus, open reading frame, silkworm-baculovirus expression system, immunogenicity, subunit vaccine

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Expression and immunological analysis of capsid protein precursor of swine vesicular disease virus HK/70

Tian

Shang

et al. 2010

Virol. Sin.

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VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability of the VP1 protein to induce an immune response was then evaluated in guinea pigs. Western blot and ELISA results indicated that the VP1 protein can be recognized by SVDV positive serum, Furthermore, anti-SVDV specific antibodies and lymphocyte proliferation were elicited and increased by VP1 protein after vaccination. These results encourage further work towards the development of a vaccine against SVDV infection.

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Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

Cited by 3 publications

References 29 publications

Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain

Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain

Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae

Expression and immunological analysis of capsid protein precursor of swine vesicular disease virus HK/70

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