Infertility and venereal disease in cattle inseminated with semen containing bovine herpesvirus type 5

Kirkland, PD; Poynting, A. J.; Gu, Xiuli; Davis, R. J.

doi:10.1136/vetrec.165.4.111

Cited by 33 publications

(18 citation statements)

References 20 publications

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“…Despite numerous reports of BoHV-5 in bull semen, the significance of BoHV-5 in semen is unknown [16-20]. Recently, an association between venereal disease in cows and artificial insemination with semen contaminated with BoHV-5 has been described in Australia [18,21]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5

Brenner

Silva-Frade

Ferrarezi

et al. 2012

Reprod Biol Endocrinol

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BackgroundBovine Herpesvirus type-5 (BoHV-5) is a neurovirulent α-Herpesvirus which is potentially pathogenic for cows and suspected to be associated with reproductive disorders. Interestingly, natural transmission of BoHV-5 by contaminated semen was recently described in Australia. Additionally, BoHV-5 was also isolated from the semen of a healthy bull in the same country and incriminated in a natural outbreak of reproductive disease after artificial insemination. In contrast with BoHV-1, experimental exposure of in vitro produced bovine embryos to BoHV-5 does not affect embryo viability and seems to inhibit some pathways of apoptosis. However, the mechanisms responsible for these phenomena are poorly understood. In this study, we examined mitochondrial activity, antioxidant protection, stress response and developmental rates of in vitro produced bovine embryos that were exposed and unexposed to BoHV-5.MethodsFor this purpose, bovine embryos produced in vitro were assayed for cell markers after experimental infection of oocytes (n = 30; five repetitions), in vitro fertilization and development. The indirect immunofluorescence was employed to measure the expression of superoxide dismutase 1 (SOD1), anti-oxidant like protein 1 (AOP-1), heat shock protein 70.1 (Hsp 70.1) and also viral antigens in embryos derived from BoHV-5 exposed and unexposed oocytes. The determination of gene transcripts of mitochondrial activity (SOD1), antioxidant protection (AOP-1) and stress response (Hsp70.1) were evaluated using the reverse transcriptase polymerase chain reaction (RT-PCR). MitoTracker Green FM, JC-1 and Hoechst 33342-staining were used to evaluate mitochondrial distribution, segregation patterns and embryos morphology. The intensity of labeling was graded semi-quantitatively and embryos considered intensively marked were used for statistical analysis.ResultsThe quality of the produced embryos was not affected by exposure to BoHV-5. Of the 357 collected oocytes, 313 (+/− 6.5; 87,7%) were cleaved and 195 (+/− 3.2; 54,6%) blastocysts were produced without virus exposure. After exposure, 388 oocytes were cleaved into 328 (+/− 8.9, 84,5%), and these embryos produced 193 (+/− 3.2, 49,7%) blastocysts. Viral DNA corresponding to the US9 gene was only detected in embryos at day 7 after in vitro culture, and confirmed by indirect immunofluorescence assay (IFA). These results revealed significant differences (p < 0.05) between exposed and unexposed oocytes fertilized, as MitoTracker Green FM staining Fluorescence intensity of Jc-1 staining was significantly higher (p < 0.005) among exposed embryos (143 +/− 8.2). There was no significant difference between the ratios of Hoechst 33342-stained nuclei and total cells in good-quality blastocysts (in both the exposed and unexposed groups). Using IFA and reverse transcriptase polymerase chain reaction (RT-PCR) for the set of target transcripts (SOD1, AOP-1 and Hsp 70.1), there were differences in the mRNA and respective proteins between the control and exposed embryos. Only the exposed em...

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Section: Introductionmentioning

confidence: 99%

Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5

Brenner

Silva-Frade

Ferrarezi

et al. 2012

Reprod Biol Endocrinol

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“…O herpesvírus bovino -5 (BoHV-5), prevalente no Brasil (Salvador et al, 1998), tem sido primariamente associado a encefalites ou meningoencefalites em bovinos, embora já tenha sido associado a problemas reprodutivos, em alguns casos (Bratanich et al, 1991;Kirkland et al, 2009). As amostras de BoHV-5 foram também subdivididas em subtipos a, b e "não a, não b", ou "c" (D'Arce et al, 2002).…”

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Herpesvírus bovinos (BoHV-1.1 e BoHV-1.2b) em forma infecciosa em encéfalos de bovinos submetidos ao diagnóstico de raiva no estado do Rio Grande do Sul

Batista

Schmidt

Spilki

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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RESUMOVerificou-se a incidência de herpesvírus bovinos (BoHVs) em encéfalos de bovinos submetidos ao diagnóstico de raiva no estado do Rio Grande do Sul. Para tanto, amostras coletadas durante dois anos (n=70) foram submetidas ao isolamento viral em cultivos celulares. Os BoHVs foram isolados em dois (2,9%) encéfalos. Após serem submetidas à caracterização antigênica e molecular, as amostras foram subtipadas como BoHV-1.1 e BoHV-1.2b. A BoHV-1.1 foi isolada de um encéfalo que foi também positivo para raiva. O vírus da raiva foi identificado em 11 amostras (15,7%). Estes achados revelam que a incidência de BoHVs em forma infecciosa em bovinos com encefalite foi baixa, embora represente 16,7% (2/12) dos encéfalos nos quais um agente viral foi identificado. Tal fato confirma a já reportada associação entre BoHV-1 e encefalites. Esse é o primeiro relato da ocorrência de BoHV-1.2b, um subtipo considerado menos patogênico, em um caso de doença neurológica em bovinos.Palavras-chave: herpesvírus bovino-1, BoHV-1, BoHV-1.2b, raiva, encefalite ABSTRACT The incidence of bovine herpesviruses (BoHVs) was determined in brains of

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“…Bovine herpesvirus 1 has been associated with respiratory tract infections (bovine infectious rinotracheitis, IBR), abortions, sporadic cases of encephalitis and reproductive tract infections (vulvovaginitis or balanopostitis) (Engels and Ackermann 1996, Muylkens et al 2007, Nandi et al 2009. Bovine herpesvirus 5 seems to be widely distributed in Latin America (Esteves et al 2008, Pidone et al 1999, Silva et al 2007, although it has been occasionally found in other regions of the world (Bartha et al 1969, Carrillo et al 1983, Diallo et al 2010, d'Offay et al 1993, Eugster et al 1975, Kirkland et al 2009. Bovine herpesvirus 5 has been associated with encephalitis and meningoencephalitis in calves and is of utmost importance in the differential diagnosis of bovine spongiform encephalitis .…”

Section: Introductionmentioning

confidence: 99%

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

Furtado¹,

Campos

Llambí³

et al. 2014

Arch. med. vet.

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Infertility and venereal disease in cattle inseminated with semen containing bovine herpesvirus type 5

Cited by 33 publications

References 20 publications

Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5

Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5

Herpesvírus bovinos (BoHV-1.1 e BoHV-1.2b) em forma infecciosa em encéfalos de bovinos submetidos ao diagnóstico de raiva no estado do Rio Grande do Sul

Detection of bovine herpesvirus 1 and 5 in trigeminal ganglia of beef cattle in Uruguay

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