Inflammation and endothelial dysfunction during aging: role of NF-κB

Csiszár, Anna; Wang, Mingyi; Lakatta, Edward G.; Ungvári, Zoltán

doi:10.1152/japplphysiol.90470.2008

Cited by 394 publications

(319 citation statements)

References 111 publications

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“…Comparisons among groups were performed by two-way analysis of variance (ANOVA), using the Sigma Stat program (Jandel Scientific). The IC 50 and maximum dilation response (E max ) values from the concentration-response curves of ACh for relaxation of the rat aorta were performed using the Sigma Plot (Systat Software, San Jose, CA, USA) program. Differences were considered statistically significant when P<0.05.…”

Section: Resultsmentioning

confidence: 99%

Non-steroidal anti-inflammatory drugs attenuate the vascular responses in aging metabolic syndrome rats

et al. 2014

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Aim: Metabolic syndrome (MS) and aging are low-grade systemic inflammatory conditions, and inflammation is a key component of endothelial dysfunction. The aim of this study was to investigate the effects of non-steroidal anti-inflammatory drugs (NSAIDs) upon the vascular reactivity in aging MS rats. Methods: MS was induced in young male rats by adding 30% sucrose in drinking water over 6, 12, and 18 months. When the treatment was finished, the blood samples were collected, and aortas were dissected out. The expression of COX isoenzymes and PLA 2 in the aortas was analyzed using Western blot analysis. The contractile responses of aortic rings to norepinephrine (1 μmol/L) were measured in the presence or absence of different NSAIDs (10 μmol/L for each).Results: Serum levels of pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β) in control rats were remained stable during the aging process, whereas serum IL-6 in MS rats were significantly increased at 12 and 18 months. The levels of COX isoenzyme and PLA 2 in aortas from control rats increased with the aging, whereas those in aortas from MS rats were irregularly increased with the highest levels at 6 months. Pretreatment with acetylsalicylic acid (a COX-1 preferential inhibitor), indomethacin (a non-selective COX inhibitor) or meloxicam (a COX-2 preferential inhibitor) decreased NE-induced contractions of aortic rings from MS rats at all the ages, with meloxicam being the most potent. Acetylsalicylic acid also significantly reduced the maximum responses of ACh-induced vasorelaxation of aortic rings from MS rats, but indomethacin and meloxicam had no effect. Conclusion: NSAIDs can directly affect vascular responses in aging MS rats. Understanding the effects of NSAIDs on blood vessels may improve the treatment of cardiovascular diseases and MS in the elders.

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Section: Resultsmentioning

confidence: 99%

Non-steroidal anti-inflammatory drugs attenuate the vascular responses in aging metabolic syndrome rats

et al. 2014

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“…The relationship between CLAIS and hypertension is likely due to both direct AGE deposition in the vasculature which contributes to arterial stiffening as well as impaired capacity of the endothelium to generate vasodilating factors [34,36]. The nuclear factor-kappa B (NF-κB) pathway is considered to be a major intracellular inflammatory pathway which is also known to mediate many of the vascular inflammatory processes [34,36,37]. AGEs are thought to activate the NF-κB pathway primarily as a result of their ligation with their pro-inflammatory full-length cell surface receptor RAGE [34,[38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Plasma advanced glycation end products (AGEs) and NF-κB activity are independent determinants of diastolic and pulse pressure

Sourris

Lyons

Dougherty

et al. 2014

Clinical Chemistry and Laboratory Medicine

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Background: High levels of circulating advanced glycation end products (AGEs) can initiate chronic low-grade activation of the immune system (CLAIS) with each of these factors independently associated with cardiovascular (CV) morbidity and mortality. Therefore, our objective was to characterize the relationship between serum AGEs, CLAIS and other risk factors for CV disease in normotensive non-diabetic individuals. Methods: We measured body mass index (BMI), waistto-hip ratio (WHR), blood pressure, lipid and glucose profile in 44 non-diabetic volunteers (17 female, 27 males). Carbo xymethyl-lysine (CML) was measured by ELISA as a marker for circulating AGEs and NF-κB p65 activity as an inflammatory marker by DNA-binding in peripheral blood mononuclear cells lysates (PBMC). Results: Plasma CML concentrations were related to diastolic blood pressure (r = −0.51, p < 0.01) independently of age, sex, BMI and WHR (p < 0.05). Diastolic blood pressure was also related to NF-κB activity in PBMC (r = 0.47, p < 0.01) before and after adjustment for age, sex, BMI and WHR (p < 0.05). Plasma CML concentrations were related to the pulse pressure before (r = 0.42; p < 0.05) and after adjustment for age, sex, BMI and waist (p < 0.05). Neither CML nor NF-κB activity were related to systolic blood pressure (both p = ns). Plasma CML concentrations were not associated with plasma lipid or glucose concentrations (all p = ns). Conclusions: Plasma AGE levels and NF-κB activity in PBMC were independent determinants of diastolic and pulse pressure in healthy normotensive individuals. This association suggests a role for AGEs in the etiology of hypertension, possibly via the initiation of CLAIS and aortic stiffening.

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“…A study by Sarkar et al (2004) showed that human polynuleotide phosphrylase might play a significant role in producing pathological changes associated with aging by generating proinflammatory cytokines via reactive oxidative stress (reactive oxygen species(ROS) and NF-B. The role of NF-B in bridging the explanation of how aging is associated with inflammation and endothelial dysfunction is reviewed well by Csiszar et al (2008). Another study has shown that depletion of cellular (GSH) during aging plays an important role in regulating the hepatic response to IL-1 (Rutkute et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Age is an Important Risk Factor for Type 2 Diabetes Mellitus and Cardiovascular Diseases

Suastika¹,

Dwipayana²,

Semadi³

et al. 2012

Glucose Tolerance

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Inflammation and endothelial dysfunction during aging: role of NF-κB

Cited by 394 publications

References 111 publications

Non-steroidal anti-inflammatory drugs attenuate the vascular responses in aging metabolic syndrome rats

Non-steroidal anti-inflammatory drugs attenuate the vascular responses in aging metabolic syndrome rats

Plasma advanced glycation end products (AGEs) and NF-κB activity are independent determinants of diastolic and pulse pressure

Age is an Important Risk Factor for Type 2 Diabetes Mellitus and Cardiovascular Diseases

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