Inflammatory genes in nasal polyposis

Figueiredo, Elisabete; Silva, Ismael Dale Cotrim Guerreiro; Weckx, Luc Louis Maurice

doi:10.1097/moo.0b013e3282f363f1

Cited by 24 publications

(26 citation statements)

References 28 publications

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“…TIMPs act as inhibitors of MMPs. Finally, all of these confounding and multiple regulators may ultimately contribute to tissue remodeling 334. Eosinophilic inflammation with the index by ECP can lead to the downregulation of TGF-β1 and TGF-β2, and the decreased expression of TIMP-4.…”

Section: Discussionmentioning

confidence: 99%

Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Yang

et al. 2017

Allergy Asthma Immunol Res

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PurposeWe have found that expression of γδT cells is increased in pathological mucosa of chronic rhinosinusitis with nasal polyps (CRSwNP) compared with normal nasal mucosa. This increase is correlated with the infiltration of eosinophils in CRSwNP. Here, we investigated the expression of γδT cells, inflammation and tissue remodeling factors as well as their probable relationships in different types of chronic rhinosinusitis (CRS) in China.MethodsA total of 76 surgical tissue samples that included 43 CRSwNP samples (15 eosinophilic and 28 non-eosinophilic), 17 CRS samples without nasal polyps (CRSsNP), and 16 controls were obtained. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA expression levels of Vγ1+ γδT cells, Vγ4+ γδT cells, eosinophil cationic protein (ECP), interleukin (IL)-8, transforming growth factor (TGF)-β2, metalloproteinase (MMP)-7, tissue inhibitor of metalloproteinase (TIMP)-4 and hypoxia-inducible factor (HIF)-1α. Enzyme linked immunosorbent assay (ELISA) was used to measure the protein level of ECP and MMP-7 in CRSwNP. The eosinophils were counted and the level of edema was analyzed with HE staining.ResultsThe mRNA expression levels of the Vγ1 subset, ECP and MMP-7 were significantly increased in CRSwNP with histological characteristics of eosinophilic infiltration and edema. The expression of the Vγ1 gene in CRSwNP correlated positively with the expression of both ECP and MMP-7. No significant decreases in the mRNA expression levels of TGF-β2, TIMP-4 or HIF-1α were observed in the CRSwNP samples. The expression levels of Vγ1 gene, ECP and MMP-7 were significantly increased in eosinophilic CRSwNP compared to non-eosinophilic CRSwNP.ConclusionsOur results suggest the associations between Vγ1+ γδT cells, ECP and MMP-7 in CRSwNP, indicating that Vγ1+ γδT cells can induce the eosinophilic inflammation, which has a further effect on the formation of edema.

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Section: Discussionmentioning

confidence: 99%

Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Yang

et al. 2017

Allergy Asthma Immunol Res

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“…Patients with NP typically present with nasal obstruction, hyposmia, and rhinorrhea, leading to substantial reduction in quality of life (2,3) . There has been emerging consensus that NP is a multifactorial disease leading to persistent inflammation that appears to be the common pathway (1) , due to the involvement of many inflammatory C o r r e c t e d p r o o f mediators that that could culminate in polyp formation (4,5) .…”

Section: Introductionmentioning

confidence: 99%

Interleukin-6 –174 G/C promoter gene polymorphism in nasal polyposis and asthma

Kosugi¹,

Camargo-Kosugi²,

Hirai³

et al. 2013

Rhin

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SummaryBackground: Interleukin-6 (IL-6) is an inflammatory mediator linked to nasal polyposis and asthma, with a single nucleotide polymorphism -174 G/C that seems to promote an inflammatory status. We aimed to analyze the relationship between this polymorphism and asthmatic nasal polyposis patients. Methodology:Cross-sectional study to investigate IL-6 -174 G/C genotypes of 45 nasal polyposis with asthma patients, 63 nasal polyposis-only patients, 45 asthma-only patients and 81 subjects without both diseases. Aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with specific primers followed by restriction enzyme analysis, classifying patients in GG, GC or CC genotype. Results:The GG genotype was the most frequent in all inflammatory groups. Less than 40% of controls presented with the GG genotype. There were significant differences between inflammatory groups and control group. No significant differences were seen when comparing inflammatory groups to each other, other than between nasal polyposis-only group and asthma-only group. Conclusion:The IL-6 74 GG genotype was found more frequently in all inflammatory groups than in controls. This genotype could influence nasal polyposis and asthma, and seems to be more important in the latter.

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“…Mucosal inflammation with eosinophil predominance is presumably the most significant factor in its pathogenesis. The NP stroma contains various important molecules such as cytokines, chemokines, growth factors, and adhesion molecules that all take part in the cascade of reactions that take place during NP development (2). Although Th1 cytokines are also shown to be increased in the NP stroma, NP is characterized by a Th2-dominant cytokine profile.…”

Section: Discussionmentioning

confidence: 99%

“…Although the underlying pathogenesis has been presumed to be infections, allergies, or immunological diseases, the exact pathogenesis has not been fully elucidated (1). Not all patients with chronic rhinosinusitis or allergic rhinitis have NPs; therefore, genetic predisposition such as glutathione-S-transferase genetic polymorphism, increased gene expression of chemokines, metalloproteinases, growth factors, and increased expression of leukotriene receptor genes are thought be the underlying cause of NP pathogenesis along with accompanying environmental factors (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis

İsmi¹,

Özcan²,

Polat³

et al. 2017

Turk Arch Otorhinolaryngol

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Objective: Nasal Polyp (NP) is a benign mass of the paranasal sinuses that protrudes into the nasal cavity. The exact underlying pathogenesis is not known. In this study we aimed to determine the genetic susceptibility of NP formation in relation to TNF-α-308 and IL-1β-511 promoter region gene polymorphisms. Methods:A total of 71 patients with NP with asthma (n=21) or without asthma (n=50) were taken as the study group, and 91 healthy volunteers were taken as the control group. Blood was gathered into EDTA-containing tubes, and patient DNA was extracted. The polymorphisms of the IL-β and TNF-α cytokine genes were analyzed using real time polymerase chain reaction. Results:The GG genotype in the TNF-α-308 region and the CC genotype in the IL-1β-511 region were found to be risk factors for NP formation (OR: 9.2, p=0.007 and OR: 33.3, p=0.001, respectively). Regarding allelic frequencies, the G allele at the TNF-α-308 promoter region was a risk factor for NP formation (OR: 6.06, p<0.001).Conclusion: TNF-α GG genotype in the -308 promoter region and the IL-1β CC genotype in the -511 region are genetic risk factors for NP formation.

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Inflammatory genes in nasal polyposis

Cited by 24 publications

References 28 publications

Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Interleukin-6 –174 G/C promoter gene polymorphism in nasal polyposis and asthma

TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis

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Inflammatory genes in nasal polyposis

Cited by 24 publications

References 28 publications

Vγ1+γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Vγ1+γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Interleukin-6 –174 G/C promoter gene polymorphism in nasal polyposis and asthma

TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis

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Product

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Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema

Vγ1⁺γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema