Inflammatory oedema induced by phospholipases A2 isolated from Crotalus durissus sp. in the rat dorsal skin: a role for mast cells and sensory C-fibers

Câmara, Paula Rs; Esquisatto, Laura C.M.; Camargo, Enilton A.; Ribela, M.T.C.P.; Toyama, Marcos H.; Marangoni, Sérgio; Nucci, Gilberto De; Antunes, Edson

doi:10.1016/s0041-0101(03)00037-0

Cited by 34 publications

(30 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The venom components responsible for the hepatotoxicity are still undetermined, but they probably involve PLA 2 action on cell membrane lipids and mitochondrial respiration (38), as well as various cell types such as lymphocytes (41,42), macrophages (39,40,43,44), mast cells (45), and platelets (3,(46)(47)(48)(49). In support of Coagulation disturbances (19,20) leading to fibrin deposition and ischemia, mainly through the action of the thrombin-like enzyme (gyroxin) of this venom (8,9,19) could also contribute to the hepatic damage registered in the present study.…”

Section: Discussionmentioning

confidence: 99%

Acute hepatotoxicity of Crotalus durissus terrificus (South American rattlesnake) venom in rats

França¹,

Ferrari²,

Souza³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Venom of the South American rattlesnake, Crotalus durissus terrificus (Cdt), presents myotoxic and neurotoxic outcomes, but reports on its effects on the liver are scarce. This study examined the hepatotoxicity resulting from Cdt venom administration (100, 200 and 300 μg/kg) in male Wistar rats. Animals were studies at 3, 6, 9 and 12 hours after venom injection. The hepatotoxicity was assessed through serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AP), gamma glutamyl transferase (GGT), bilirrubin and also by histopathological evaluation. All the different concentrations of Cdt venom resulted in increased levels of hepatic enzymes, when compared with the control group, except for the 100 μg/kg dose, which presented normal levels at 9 and 12 hours after venom administration. Bilirrubin levels remained unchanged by Cdt venom. Histological analysis revealed endothelial damage, inflammatory cell infiltration, as well as sinusoidal and portal congestion. Based on these observations, we may conclude that Cdt venom causes dose-and time-dependent hepatic damage in rats, characterized by elevated hepatic enzyme levels and histological alterations.

show abstract

Section: Discussionmentioning

confidence: 99%

Acute hepatotoxicity of Crotalus durissus terrificus (South American rattlesnake) venom in rats

França¹,

Ferrari²,

Souza³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…To evaluate the mechanisms involved in the edema induced by venom (10 mg/site), different drugs were administered, as follows: cyproheptadine (dual serotonin 5-hydroxytryptamine 1/2 and histamine H 1 receptor antagonist; 2 mg/kg, i.p., 30 minutes before; Câmara et al, 2003), JE 049 (bradykinin B 2 receptor antagonist; 0.6 mg/kg, i.v., 15 minutes before; Costa et al, 2001), indomethacin (nonselective COX inhibitor; 10 mg/kg, i.p., 1 hour before; Barbosa et al, 2003), L-NAME (nitric oxide synthase inhibitor; 100 nmol/site, coinjected; Ridger et al, 1997), mepyramine (histamine H 1 receptor antagonist; 6 mg/kg, i.p., 15 minutes before; Barbosa et al, 2003), SR140333 (neurokinin NK 1 receptor antagonist; 1 nmol/site, coinjected; Câmara et al, 2003), and SR48968 (neurokinin NK 2 receptor antagonist; 0.3 mmol/kg, i.v. ).…”

Section: Methodsmentioning

confidence: 99%

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Silva

Linardi

Antunes

et al. 2015

Journal of Pharmacology and Experimental Therapeutics

View full text Add to dashboard Cite

Bites by tarantulas (Theraphosidae, Mygalomorphae) in humans can result in mild clinical manifestations such as local pain, erythema, and edema. Vitalius dubius is a medium-sized, nonaggressive theraphosid found in southeastern Brazil. In this work, we investigated the mediators involved in the plasma extravasation caused by V. dubius venom in rats. The venom caused dose-dependent (0.1-100 mg/site) edema in rat dorsal skin. This edema was significantly inhibited by butyl]benzamide) (SR48968, a neurokinin NK 2 receptor antagonist) had no effect on the venom-induced increase in vascular permeability. In rat hind paws, the venom-induced edema was attenuated by ketoprofen (a nonselective COX inhibitor) administered 15 minutes postvenom. Preincubation of venom with commercial antiarachnid antivenom attenuated the venom-induced edema. These results suggest that the enhanced vascular permeability evoked by V. dubius venom involves serotonin, COX products, neurokinin NK 1 receptors, and nitric oxide formation. The attenuation of hind paw edema by ketoprofen suggests that COX inhibitors could be useful in treating the local inflammatory response to bites by these spiders.

show abstract

“…The diversity of snake venom PLA 2 functions includes: neurotoxicity, cardiotoxicity, myotoxicity, edema, hypotension, hyperalgesia as well as activation and inhibition of platelet aggregation (12)(13)(14)(15)(16)(17)(18)(19). The diversity of biological and pharmacological functions of PLA 2 denotes that accelerated or positive Darwinian evolution has occurred and appears to confer a better fitness on the snake venom (10,20).…”

Section: Introductionmentioning

confidence: 99%

Cloning of a novel acidic phospholipase A2 from the venom gland of Crotalus durissus cascavella (Brazilian northeastern rattlesnake)

Guarnieri¹,

Melo²,

Melo³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

The phospholipase A2 superfamily encompasses 15 groups that are classified into: secreted PLA2 (sPLA2); cytosolic PLA2 (cPLA2); Ca2+-independent intracellular PLA2 (iPLA2); platelet-activating factor acetylhydrolase (PAF-AH); and lysosomal PLA2. Currently, approximately 700 PLA2 sequences are known, of which 200 are obtained from the venom gland of Crotalinae snakes. However, thus far, little information is available on cloning, purification and structural characterization of PLA2 from Crotalus durisssus cascavela venom gland. In the present work, we report the molecular cloning of a novel svPLA2 from C. d. cascavella (Cdc), a predominant rattlesnake subspecies in northeastern Brazil. The Cdc svPLA2 cDNA precursor is 689 nucleotides long and encodes a protein of 138 amino acid residues, with a calculated molecular mass of approximately 13,847 Da and an estimated isoelectric point of 5.14. Phylogenetic analysis of Crotalinae PLA2 reveals that Cdc PLA2 clustered with other acidic type IIA PLA2 homologues is also present in the venom of North American rattlesnakes. Hitherto, this study presents a novel PLA2 cDNA precursor from C. d. cascavella and data reported herein will be useful for further steps in svPLA2 purification and analysis

show abstract

Inflammatory oedema induced by phospholipases A2 isolated from Crotalus durissus sp. in the rat dorsal skin: a role for mast cells and sensory C-fibers

Cited by 34 publications

References 37 publications

Acute hepatotoxicity of Crotalus durissus terrificus (South American rattlesnake) venom in rats

Acute hepatotoxicity of Crotalus durissus terrificus (South American rattlesnake) venom in rats

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Cloning of a novel acidic phospholipase A2 from the venom gland of Crotalus durissus cascavella (Brazilian northeastern rattlesnake)

Contact Info

Product

Resources

About