Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis

Cantiga-Silva, Cristiane; Estrela, Carlos; Segura-Egea, Juan José; Azevedo, Jaquelyne Poliszuk de; Oliveira, Pedro Henrique Chaves de; Cardoso, C B M; Pinheiro, Tiago Novaes; Ervolino, Edílson; Sivieri-Araújo, Gustavo; Cintra, Luciano Tavares Ângelo

doi:10.1111/iej.13519

Cited by 15 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sample size was estimated based on data from a previous study (Cantiga‐Silva et al, 2021; Dal‐Fabbro et al, 2019) considering the number of animals per group. Using an alpha error of 5% and 95% power, to recognize a significant difference of 1 in the median score, a minimum of seven animals per group was necessary.…”

Section: Methodsmentioning

confidence: 99%

“…The induction of periapical lesions was performed under general anaesthesia with the association of ketamine 75 mg/kg (Cetamin 10%, Syntec Brasil) and Xylazine 25 mg/kg (Xilazin 2%, Syntec Brasil), intramuscularly. The dental pulp of the lower right first molar was exposed to the oral environment with the aid of a carbon steel Ln Long Neck drill (Dentsply Sirona/Maillefer, Ballaigues, Switzerland) for a period of 30 days, to enable periapical lesion formation (Cantiga‐Silva et al, 2021; Cintra et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…At 10× magnification, the exit of the apical foramen was identified. At 400× magnification, cells were counted in an area encompassing the foramen exit region and the periapical tissue, assigning the following scores: no inflammation (score 1: 0 or few inflammatory cells), mild inflammation (score 2: <25 inflammatory cells), moderate inflammation (score 3: 25–125 inflammatory cells) and severe inflammation (score 4: >125 inflammatory cells; Cantiga‐Silva et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Inflammatory profile of apical periodontitis exacerbated by cigarette smoke inhalation: Histological and immunohistochemical analysis in rats

et al. 2023

Self Cite

View full text Add to dashboard Cite

Aim:The aim of this study was to evaluate the effects of cigarette smoke inhalation (CSI) on inflammation, pro-inflammatory mediators and haematological parameters in rats with induced apical periodontitis (AP).Methodology: Thirty-two 3-month-old male Wistar rats were divided into four experimental groups (n = 8): C-Control; S-rats with CSI; AP-rats with AP; and SAP-rats with CSI + AP. Animals in groups S and SAP inhaled cigarette smoke by remaining inside a smoking chamber for 8 min, three times daily, for 50 days. After 20 days of smoke inhalation, animals in AP and SAP groups had the pulps of the lower right first molar exposed to oral environment for 30 days to induce AP. In these subsequent 30 days, animals in group S and SAP continued with CSI. On Day 50, animals were euthanized and mandibles were histologically processed to assess inflammatory infiltrate, immunohistochemical interleukins (IL-1β, IL-6 and TNF-α), and blood samples collected for laboratory analysis. The Mann-Whitney test was performed for non-parametric data and the pairwise analyses of Student's t-test for parametric data, with a significance level of p < .050. Results: Inflammatory infiltrate was moderate in AP group and more severe in the SAP (p = .010). The interleukins IL-6, IL-1β and TNF-α were higher in SAP group (p < .001) when compared to the AP group. A greater number of red blood cells (p = .010), haemoglobin (p = .007) and neutrophils (p = .014) were observed in the SAP group in comparison with the AP group. Conclusion:Cigarette smoke inhalation induced a more severe inflammatory infiltrate, with increased levels of pro-inflammatory cytokines and changes in haematological parameters in rats with induced AP. Thus, CSI aggravated AP, exacerbating the inflammatory response.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Inflammatory profile of apical periodontitis exacerbated by cigarette smoke inhalation: Histological and immunohistochemical analysis in rats

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Histopathologic, histometric and immunohistochemical analyses were performed by a single-certified histologist (E.E. ), calibrated and blinded using a light microscope (DM 4000 B; Leica) and a colour camera (DFC 500; Leica) in a blinded manner (Cantiga-Silva et al, 2021). The distal root was standardized for all analyses (Dal-Fabbro et al, 2019).…”

Section: Histopathological and Immunohistochemical Analysismentioning

confidence: 99%

Curcumin reduces inflammation in rat apical periodontitis

et al. 2022

Self Cite

View full text Add to dashboard Cite

Aim The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP). Methodology Forty male Wistar rats weighing 250–280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure‐induced apical periodontitis (AP) and rats with pulp exposure‐induced apical periodontitis treated with curcumin (AP‐CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro‐CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF‐α), interleukin (Il)‐6 and Il‐1β. Tartrate‐resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal–Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05. Results Micro‐CT revealed no statistically significant differences in bone resorption between the AP and AP‐CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF‐α, Il‐6 and Il‐1β were significantly higher in the periapical lesions of the AP group than in the AP‐CUR group (p < .05). The number of TRAP‐positive multinucleated cells was higher in the AP group than in the AP‐CUR group (p < .05). In OCN‐positive cells, no differences were observed between the AP and AP‐CUR groups (p > .05). Conclusions Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti‐inflammatory effect of curcumin on AP development.

show abstract

“…However, the number of animals per group precludes any conclusions about the finding. On the other hand, recently, Cantiga-Silva et al investigated the effects of liver fibrosis on pro-inflammatory mediators and on periapical bone resorption of AP in rats [107]. The results showed a more intense inflammatory infiltrate, a greater amount of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and increased periapical bone resorption in the presence of hepatic fibrosis [107].…”

Section: Hepatic Diseasesmentioning

confidence: 99%

Evolution of endodontic medicine: a critical narrative review of the interrelationship between endodontics and systemic pathological conditions

et al. 2021

Self Cite

View full text Add to dashboard Cite

Endodontics has gained emphasis in the scientific community in recent years due to the increase in clinical and in animal models studies focused on endodontic medicine, which aims to evaluate the interrelationship between systemic and periapical tissues pathological conditions. These studies have shown that systemic changes can boost the pathogenesis of endodontic infection, favoring its development and progression. A contrary relationship is reported in numerous studies that affirm the potential of endodontic infection to trigger systemic damage and may lead to the worsening of pre-existing pathologies. Recently, the potential of filling materials to develop systemic changes such as neurological alterations had been evaluated, also showing that systemic diseases can negatively influence tissue responses to filling materials after endodontic treatment. Despite advances in endodontic medicine studies, there are still gaps in knowledge on the mechanisms of interactions between apical periodontitis (AP) and systemic diseases and much research to be done. In this sense, this critical narrative literature review aimed to show the evolution of studies in endodontic medicine to help the endodontist to know the role of systemic diseases in the pathogenesis of AP and the possible interference in the repair of periapical tissues after endodontic treatment, as well as to evidence the systemic complications that can be triggered or aggravated in the presence of endodontic infection.

show abstract

Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis

Cited by 15 publications

References 36 publications

Inflammatory profile of apical periodontitis exacerbated by cigarette smoke inhalation: Histological and immunohistochemical analysis in rats

Inflammatory profile of apical periodontitis exacerbated by cigarette smoke inhalation: Histological and immunohistochemical analysis in rats

Curcumin reduces inflammation in rat apical periodontitis

Evolution of endodontic medicine: a critical narrative review of the interrelationship between endodontics and systemic pathological conditions

Contact Info

Product

Resources

About