Inflammatory Response to a Titanium Surface with Potential Bioactive Properties: An In Vitro Study

Göransson, Anna; Gretzer, Christina; Johansson, Anna; Sul, Young‐Taeg; Wennerberg, Ann

doi:10.1111/j.1708-8208.2006.00021.x

Cited by 13 publications

(18 citation statements)

References 34 publications

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“…Taking into account the fact that primary and non-stimulated cell cultures were used and that monocytes are comprehensively differentiated cells, these results were predictable. These findings are in agreement with a prior study, 11 which observed a decrease in the total number of viable cells on all titanium surfaces over time.…”

supporting

confidence: 93%

“…Though the effects of nano-and microrough titanium topography on osteoblast behavior have been extensively described, [18][19] assays in which monocyte/macrophage behavior is examined are useful determinants for elucidating several aspects of cell interactions with implant biomaterials. 6,11,20 Mononuclear cells, especially monocytes/macrophages, were chosen for this research due to their pivotal role in wound healing, 8 producing a range of molecules which have the potential to modulate the repair process, driving the duration and intensity of the inflammatory response. 20 According to this perspective, it is reasonable to hypothesize that the interaction between mononuclear cells and the implant surface influences the subsequent phases of de novo bone formation.…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that the immersion in plasma proteins before cell seeding may have modified the interaction between monocytes/macrophages and the titanium surfaces. Furthermore, it is important to address that the decision to use the turned titanium surface as the control group in accordance with Göransson et al 11 According to Gretzer et al, 8 macrophages cultured on smooth titanium surfaces did not show differences regarding this parameter when compared to a polystyrene surface.…”

mentioning

confidence: 94%

“…1,6 Studies conducted with mononuclear cells have demonstrated substrate-dependent effects in cell activation. 1,5,[8][9]11 Once a biomaterial surface able to accelerate the heal-…”

Section: Introductionmentioning

confidence: 99%

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Effect of titanium surface on secretion of IL1β and TGFβ1 by mononuclear cells

et al. 2011

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Mononuclear cells play an important role in the modulation of healing. The characteristics of implant surface topography may alter the production of signaling molecules such as cytokines. The aim of this in vitro study was to evaluate the effects of commercially available titanium surface treatments on both cell viability and the secretion of the antagonist cytokines, IL1β and TGFβ1. Human mononuclear cells were cultured on 10 mm diameter commercially pure titanium (cpTi) disks that were prepared using a turning procedure (control=machined surface) and either acid etched or bio-anodized for 1-7 days. Adhered cells were investigated with respect to cell viability using an MTT assay, and cytokine production was verified using an ELISA assay. The results indicate that surface characteristics did not alter the cell viability at days 1 and 4, although the machined surface presented the highest absorbance values at day 7 (p = 0.0084). Cell viability was reduced throughout the time course for all analyzed surfaces (p < 0.05). On day 4, IL1β levels were significantly higher on bio-anodized compared to acid etched surfaces (p = 0.0097). TGFβ1 did not show differences among the surfaces at days 1 and 4. The responses of non-stimulated mononuclear cells to titanium surfaces suggest only modest effects of the surface treatment and roughness on pro-inflammatory cytokine (IL1β) release.

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supporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 94%

“…1,6 Studies conducted with mononuclear cells have demonstrated substrate-dependent effects in cell activation. 1,5,[8][9]11 Once a biomaterial surface able to accelerate the heal-…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of titanium surface on secretion of IL1β and TGFβ1 by mononuclear cells

et al. 2011

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“…Recent in vivo studies have shown that nanostructured implant surfaces enhance bone responses, as measured both histologically and biomechanically (Bjursten et al, 2010;Jimbo et al, 2011b). The beneficial effects of these alterations may stem from conversion of the material to a highly bioactive state because of modifications at the nano level (Goransson et al, 2006;Frojd et al, 2011), which is the core concept of the biologically inspired biomimetic engineering (Flammang and Porter, 2011).…”

Section: Introduction Tmentioning

confidence: 99%

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

et al. 2011

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Nanostructured calcium phosphate (CaP) has been histologically and biomechanically proven to enhance osseointegration of implants; however, conventional techniques were not sufficiently sensitive to capture its biological effects fully. Here, we compared the conventional removal torque (RTQ) evaluation and gene expression in tissues around nanostructured CaP-coated implants, using real-time RT-PCR, with those of uncoated implants, in a rabbit model. At 2 wks, RTQ values were significantly higher, alkaline phosphatase (ALP) expression was significantly higher, and runt-related transcription factor 2 and tumor necrosis factor-α expressions were significantly lower in the coated than in the uncoated implants. This indicates that inflammatory responses were suppressed and osteoprogenitor activity increased around the CaP-coated surface. At 4 wks, although RTQ values did not significantly differ between the 2 groups, ALP and osteocalcin (OCN) were significantly up-regulated in the coated group, indicating progressive mineralization of the bone around the implant. Moreover, an osteoclast marker, adenosine triphosphatase, which indicates acidification of the resorption lacunae, was significantly higher for the coated implants, suggesting gradual resorption of the CaP coating. This study reveals detailed genetic responses to nanostructured CaP-coated implants and provides evidence that the effect of nanotopography is significant during the osseointegration cascade.

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Effects of locally administered insulin on bone formation in non‐diabetic rats

Malekzadeh

Tengvall

Öhrnell

et al. 2012

J Biomedical Materials Res

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The possibility to control bone formation would be favorable in many areas of medicine, where bone defects is still a major challenge. Insulin has been suggested to exert both systemic and local anabolic effects in bone tissues. This raised the question whether locally administrated insulin could provide new therapeutic strategies for patients with local bone defects and impaired bone healing. The aim of this study was to evaluate bone formation in non-diabetic rats when local insulin is administered. This study differs from previous reports in two aspects: the use of non-diabetic animals and locally administered insulin. Twenty-four implants were inserted into 12 rats-one insulin-coated and one control-in each tibia for four weeks. Interferometry and histomorphometry were used to evaluate the surface topography and bone formation, respectively. Results demonstrated no significant changes in surface topography after insulin immobilization. Histomorphometry revealed significantly more bone around the insulin-coated implants (BA) (p = 0.005) and a similar amount of bone at the implant surface (BIC) (p = 0.117) compared with the controls. It was concluded that locally administered insulin from a titanium implant surface has the potential to increase bone formation not only in diabetic subjects but also in non-diabetic subjects.

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Inflammatory Response to a Titanium Surface with Potential Bioactive Properties: An In Vitro Study

Cited by 13 publications

References 34 publications

Effect of titanium surface on secretion of IL1β and TGFβ1 by mononuclear cells

Effect of titanium surface on secretion of IL1β and TGFβ1 by mononuclear cells

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

Effects of locally administered insulin on bone formation in non‐diabetic rats

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