2018
DOI: 10.1016/j.ejpb.2018.07.019
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Influence and effect of osmolytes in biopharmaceutical formulations

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Cited by 36 publications
(25 citation statements)
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“…Moreover, due to its favorable biological and chemical properties (non-hygroscopic, biocompatible, biodegradable, etc. ), mannitol is a basic product widely used in chemical, pharmaceutical, and medical industries, such as in pharmaceutical formulations of chewable tablets and granulated powders [149], mannitol-based polyesters [150], and an osmotic diuretic for intoxication therapy [151]. Additionally, mannitol was found to be effective as an innovative carbon source in the partial nitrification process for wastewater treatment, facilitating nitrogen removal while remarkably reducing N2O emissions [152].…”
Section: Resultsmentioning
confidence: 99%
“…Moreover, due to its favorable biological and chemical properties (non-hygroscopic, biocompatible, biodegradable, etc. ), mannitol is a basic product widely used in chemical, pharmaceutical, and medical industries, such as in pharmaceutical formulations of chewable tablets and granulated powders [149], mannitol-based polyesters [150], and an osmotic diuretic for intoxication therapy [151]. Additionally, mannitol was found to be effective as an innovative carbon source in the partial nitrification process for wastewater treatment, facilitating nitrogen removal while remarkably reducing N2O emissions [152].…”
Section: Resultsmentioning
confidence: 99%
“…Osmolytes, namely disaccharides such as sucrose and trehalose, are known to favor the native structure of proteins as consequence of a nonspecific compaction effect on the native as well as on the unfolded state. 21,[26][27][28] Glycerol also stabilizes the native state and enhances the hydrophobic collapse (increased compaction) of the unfolded state toward the native state as shown at the single molecule level for ubiquitin. 29 The slowdown of protein deactivation that occurs often in the presence of osmolytes as observed here, in most of the cases, results from the shift in the equilibrium toward the native structure driven by the osmolyte-induced compaction effect.…”
Section: Discussionmentioning
confidence: 99%
“…Sucrose and Larginine as representatives of the carbohydrate and amino acid classes, respectively, and 2 of the most used osmolytes in biopharmaceutical formulations. 21 Glycerol was used as representative of the polyol class. The presence of 0.6 M sucrose increases the half-life time to 230 h and 1 M sucrose prevents completely PDIA1 deactivation for more than 200 h (Fig.…”
Section: Cold Stability Of Protein Disulfide Isomerase Measured By Ismentioning
confidence: 99%
“…According to the vitrification theory, the protein is immobilized in a rigid, amorphous glassy sugar matrix, preventing molecular mobility (Ohtake et al, 2011). Otherwise, according to the water replacement theory, hydrogen bonds between the protein and the hydroxyl groups of the sugars are formed upon drying replacing hydrogen bonds between water and the protein, contributing to protein stabilization (Wlodarczyk et al, 2018).…”
Section: L-asparaginase Formulationmentioning
confidence: 99%