Influence du rapport molaire phosphatidylcholines/cholestérol des liposomes sur la réactivité du cholestérol à la cholestérol : oxygène oxydoréductase

Peynet, Jacqueline; Delattre, Jacques; Canal, J; Rousselet, F; Girard, M.

doi:10.1016/s0300-9084(79)80205-9

Cited by 3 publications

(5 citation statements)

References 22 publications

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“…Early studies demonstrated that the rate of cholesterol oxidation was highest with nonionic detergent micelles [e.g. Triton X‐100 (polyethylene glycol octylphenyl ether) or Thesit (polyethylene glycol monododecyl ether)] containing cholesterol [24,25]. The elements of the kinetic model are the same with detergent micelles.…”

Section: Membrane Effects On Cholesterol Oxidase Activitymentioning

confidence: 99%

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Cholesterol oxidase: physiological functions

Kreit¹,

Sampson²

2009

The FEBS Journal

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An important aspect of catalysis performed by cholesterol oxidase (3β‐hydroxysteroid oxidase) concerns the nature of its association with the lipid bilayer that contains the sterol substrate. Efficient catalytic turnover is affected by the association of the protein with the membrane as well as the solubility of the substrate in the lipid bilayer. In this review, the binding of cholesterol oxidase to the lipid bilayer, its turnover of substrates presented in different physical environments, and how these conditions affect substrate specificity, are discussed. The physiological functions of the enzyme in bacterial metabolism, pathogenesis and macrolide biosynthesis are reviewed in this context.

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Section: Membrane Effects On Cholesterol Oxidase Activitymentioning

confidence: 99%

“…Moreover, the k cat */K m * varies depending on the multibody interactions in the membrane. When the mole fraction of cholesterol exceeds a sustainable packing ratio [6,19–21], the excess cholesterol is a better substrate [7,25,40,43]. Restated, cholesterol can exist in membranes as free cholesterol clusters.…”

Section: Enzyme Substrate Specificitymentioning

confidence: 99%

Cholesterol oxidase: physiological functions

Kreit¹,

Sampson²

2009

The FEBS Journal

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“…5-Cholesten-3-one is therefore implicated as an intermediate in this sequence. The substrate specificity of the cholesterol oxidase from Nocardia erythropolis for 3f8-hydroxy steroid oxidation has been studied in some detail , 1976aWortberg, 1975;Peynet et al, 1976). The length and type of the side chain at position C-17 of a 3,8-hydroxy steroid were found to influence considerably the rate of oxidation.…”

mentioning

confidence: 99%

The substrate specificity and stereochemistry, reversibility and inhibition of the 3-oxo steroid Δ4-Δ5-isomerase component of cholesterol oxidase

Smith¹,

Brooks²

1977

Biochemical Journal

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1. 5-Cholesten-3-one was shown to be an intermediate in the conversion of cholesterol into 4-cholesten-3-one by Nocardia cholesterol oxidase. 2. The absence of a C-17 side chain from 5-androstene-3,17-dione slightly increased the Vmax. of the isomerase activity relative to 5-cholesten-3-one (1.7-fold), but greatly increased the Km. 3. Incubations of [4alpha-2H]-and [4beta-2H]-cholesterol with cholesterol oxidase showed that the 4beta-hydrogen atom can be transferred to the 6beta-position. However, incubations of cholesterol, 5-cholesten-3-one and 4-cholesten-3-one with the enzyme in 2H2O led to some incorporation of 2H into the 4-cholesten-3-one products, mostly at position 6beta. 4. Both the isomerase and the oxidase activities of cholesterol oxidase were inhibited by 5,10-seco-19-nor-5-cholestyne-3,10-dione.

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“…The specificity of the enzymic method depends upon that of the cholesterol oxidase (16,17) extracted from Nocardia erythropolis. This enzyme has a wide spectrum: it also partially oxidises Δ 7-cholestanol (15,16,17,18), and fully oxidises cholestanol arid 7-dehydrocholesterol (15). In human plasma these structural analogues of cholesterol are present at an average level of 0.05 mmol/1 (20 mg/1) for Δ 7-cholestanol, 0.10 mmol/1 (40 mg/1) for cholestanol, and 0.52 mmol/1 (200 mg/1) for 7-dehydroeholesterol.…”

Section: Calculationmentioning

confidence: 99%

“…Some studies have already demonstrated its greater specificity. Among the numerous derivatives of cholesterol, only cholestanol has the same retention time as cholesterol, and it is therefore the only plasma constituent that interferes in this method (15)(16)(17)(18)(19)(20)(21)(22)(23).…”

Section: Calculationmentioning

confidence: 99%

Enzymic Assay of Plasma Cholesterol: A Comparison of Analytical Variations Found Using the Greiner GSA II and the Technicon SMA 12/60 and SMA II

Steinmetz¹,

E²,

Gaspart³

1979

Clinical Chemistry and Laboratory Medicine

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Summary: Cholesterol was assayed on the Greiner GSA II and the Technicon SMA 12/60 and SMA II autoanalyzers, vnthAllairfs entirely enzymic method (Allain et al. (1974), Clin. Chem. 20,470-475). The coefficients of variation in the results over a period of one month were less than 6% with the GSA II and the SMA II, but up to 9% on the SMA 12/60. Although the accuracy of the enzymic method depends on the specificity of the cholesterol oxidase and cholesterol esterase, the results were in good agreement with those obtained byAbelPs method (Abell et al. (1952) Allain et al. ((1974), Clin. Chem. 20, 470-475) bestimmt. Die Variationskoeffizienten der ber einen Zeitraum von einem Monat erhaltenen Ergebnisse waren < 6% mit dem GSA II und dem SMA II, aber bis zu 9% mit dem SMA 12/60. Obwohl die Richtigkeit der enzymatischen Methode von der Spezifit t der Cholesterinoxidase und -esterase abh ngt, stimmen die Ergebnisse gut mit denen berein, die mit der Methode von Abell et al. ((1952), J. Biol. Chem. 795, 357-366) an frischem oder lyophilisiertem Plasma von Menschen erhalten wurden. Die Cholestefinkonzentration war bei 4 °C oder in gefrorenem oder lyophilisiertem Plasma stabil. Keine der gepr ften Pharmaka st rten den enzymatischen Test.

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Influence du rapport molaire phosphatidylcholines/cholestérol des liposomes sur la réactivité du cholestérol à la cholestérol : oxygène oxydoréductase

Cited by 3 publications

References 22 publications

Cholesterol oxidase: physiological functions

Cholesterol oxidase: physiological functions

The substrate specificity and stereochemistry, reversibility and inhibition of the 3-oxo steroid Δ4-Δ5-isomerase component of cholesterol oxidase

Enzymic Assay of Plasma Cholesterol: A Comparison of Analytical Variations Found Using the Greiner GSA II and the Technicon SMA 12/60 and SMA II

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