Influence of 2,4-d and lactose on pollen embryogenesis in anther culture of potato

Abstract

Anthers of diploid genotypes of Solanum tuberosum capable of androgenesis were cultured on different media to examine the effect on induction of pollen embryogenesis of 2,4-D and lactose. Anthers cultured in callogenic medium with 2,4-D and sucrose produced pollen derived embryoids only exceptionally. When sucrose was replaced by lactose the frequency of embryogenesis was as high or higher than in embryogenic auxin-free medium. Substitution of lactose for sucrose in the embryogenic medium had no effect. Supplementing the embryogenic medium with 2,4-0 strongly reduced the frequency of pollen embryoids in the presence of sucrose but not with lactose.Abbreviations: 2,4-D -2,4-dichlorophenoxyacetic acid

Effect of genotype and medium composition on flax Linum usitatissimum L. anther culture

“…Lactose significantly increased shoot regeneration for 99-182-F 1 , but significantly decreased shoot regeneration for 99-179-F 1 . The beneficial effect of replacing sucrose with lactose in embryo induction from potato anther culture was previously reported (Ríhová and Tupý 1996). In comparison with 9% sucrose, medium containing 9% maltose increased callus induction for two of the three genotypes tested.…”

Aspectos básicos y aplicados de la inducción de embriogénesis en microsporas de pimiento y colza

“…De los cinco principales cultivos de solanáceas (pimiento, tabaco, patata, berenjena y tomate), únicamente tabaco y patata pueden considerarse no recalcitrantes. En tabaco existen desde hace años sistemas puestos a punto para obtener DHs a partir de cultivos de antera y microsporas aisladas con una eficiencia aceptable (Chupeau et al, 1998;Touraev et al, 2001;Maluszynski et al, 2003) y en patata se han empleado protocolos para inducir la embriogénesis mediante cultivos de microsporas aisladas (Rihova y Tupy, 1999). El resto de solanáceas siguen estando consideradas como recalcitrantes, porque a pesar de que en algunos casos se han obtenido DHs, como en berenjena (Miyoshi, 1996; Corral-Martínez y Seguí-Simarro, 2014) y pimiento (Kim et al, 2008Lantos et al, 2009 , todavía se está muy lejos de la eficiencia obtenida en tabaco o colza.…”