Influence of a novel calcium-phosphate coating on the mechanical properties of highly porous collagen scaffolds for bone repair

Al-Munajjed, Amir A.; O’Brien, Fergal J.

doi:10.1016/j.jmbbm.2008.05.001

Cited by 69 publications

(43 citation statements)

References 35 publications

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“…For example, the toughness of bone is achieved by the interplay of organic collagen network that supports the inorganic mineral phase [59]. Given the low mechanical properties of collagen-based scaffolds, many studies have combined collagen with calcium phosphates to develop biomimetic scaffolds with improved mechanical properties [50,60,61]. Micron-sized HA (m-HA) particles can lead to poor resorbability and brittle constructs.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Physical and biological characteristics of nanohydroxyapatite and bioactive glasses used for bone tissue engineering

Yousefi

Oudadesse

Akbarzadeh

et al. 2014

Nanotechnology Reviews

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Critical-sized bone defects have, in many cases, posed challenges to the current gold standard treatments. Bioactive glasses are reported to be able to stimulate more bone regeneration than other bioactive ceramics; however, the difficulty in producing porous scaffolds made of bioactive glasses has limited their extensive use in bone regeneration. On the other hand, calcium phosphate ceramics such as synthetic hydroxyapatite and tricalcium phosphate are widely used in the clinic, but they stimulate less bone regeneration. This paper gives an overview of the recent developments in the field of bioactive nanoparticles, with a focus on nanohydroxyapatite and bioactive glasses for bone repair and regeneration. First, a brief overview of the chemical structure and common methods used to produce synthetic nanohydroxyapatite and bioactive glasses has been presented. The main body of the paper covers the physical and biological properties of these biomaterials, as well as their composites with biodegradable polymers used in bone regeneration. A summary of existing challenges and some recommendations for future directions have been brought in the concluding section of this paper.

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Section: Mechanical Propertiesmentioning

confidence: 99%

Physical and biological characteristics of nanohydroxyapatite and bioactive glasses used for bone tissue engineering

Yousefi

Oudadesse

Akbarzadeh

et al. 2014

Nanotechnology Reviews

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“…Novel lyophilisation techniques developed in our laboratory have enabled the fabrication of a series of collagen-based scaffolds with pore size, porosity and mechanical properties tailored specifically for bone tissue regeneration [32][33][34][35][36][37][38]. The composition of these scaffolds has been adapted to contain various constituents such as, for example, glycosaminoglycans (GAG) which effectively increases cellular attachment, migration and infiltration throughout the matrix.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The development of non-viral gene-activated matrices for bone regeneration using polyethyleneimine (PEI) and collagen-based scaffolds

Tierney

Duffy

Hibbitts

et al. 2012

Journal of Controlled Release

124

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The healing potential of scaffolds for tissue engineering can be enhanced by combining them with genes to produce gene-activated matrices (GAMs) for tissue regeneration. We examined the potential of using polyethyleneimine (PEI) as a vector for transfection of mesenchymal stem cells (MSCs) in monolayer culture and in 3D collagen-based GAMs. PEI-pDNA polyplexes were fabricated at a range of N/P ratios and their optimal transfection parameters (N/P 7 ratio, 2µg dose) and transfection efficiencies (45 ± 3%) determined in monolayer culture. The polyplexes were then loaded onto collagen, collagen-glycosaminoglycan and collagen-nanohydroxyapatite scaffolds where gene expression was observed up to 21 days with a polyplex dose as low as 2µg. Transient expression profiles indicated that the GAMs act as a polyplex depot system whereby infiltrating cells become transfected over time as they migrate throughout the scaffold. The collagen-nHa GAM exhibited the most prolonged and elevated levels of transgene expression. This research has thus demonstrated that PEI is a highly efficient pDNA transfection agent for both MSC monolayer cultures and in the 3D GAM environment. By combining therapeutic gene therapy with highly engineered scaffolds, it is proposed that these GAMs might have immense capability to promote tissue regeneration.

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“…Collagen is used extensively as a scaffold biomaterial due to its biocompatible and biodegradable properties [8][9][10][11][12]. From an orthopedic perspective however, collagen scaffolds are limited by their poor mechanical characteristics and for this reason many studies, including research in our lab, have combined collagen with calcium phosphates to improve their mechanical properties [13][14][15][16]. However, poor resorbability and brittle constructs are problems that occur when using micron-sized HA particles [17,18].…”

Section: Introductionmentioning

confidence: 99%

Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering

Cunniffe

Dickson

Partap

et al. 2009

J Mater Sci: Mater Med

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173

146

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Influence of a novel calcium-phosphate coating on the mechanical properties of highly porous collagen scaffolds for bone repair

Cited by 69 publications

References 35 publications

Physical and biological characteristics of nanohydroxyapatite and bioactive glasses used for bone tissue engineering

Physical and biological characteristics of nanohydroxyapatite and bioactive glasses used for bone tissue engineering

The development of non-viral gene-activated matrices for bone regeneration using polyethyleneimine (PEI) and collagen-based scaffolds

Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering

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