Influence of a virus infection (densonucleosis) on the formation of uric acid in the fat body of Galleria mellonella

Smirnoff, W. A.; Loiselle, Jean-Marie

doi:10.1016/0022-2011(69)90175-x

Cited by 5 publications

(1 citation statement)

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“…In addition, the UA metabolism pathway where MoCoS is located has been reported to be closely related to viruses. For example, BmNPV infection can cause significant changes in key proteins of the UA pathway in silkworms (Matindoost et al, 2008), and it has also been reported that the UA content in Melonella mellonella larvae infected with dense nuclear disease is significantly higher than that in healthy larvae (Smirnoff & Loiselle, 1969).…”

Section: Discussionmentioning

confidence: 99%

Studying the role of Bombyx mori molybdenum cofactor sulfurase in Bombyx mori nucleopolyhedrovirus infection

Lv,

Lai,

Gong

et al. 2024

Insect Molecular Biology

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Molybdenum cofactor sulfurase (MoCoS) is a key gene involved in the uric acid metabolic pathway that activates xanthine dehydrogenase to synthesise uric acid. Uric acid is harmful to mammals but plays crucial roles in insects, one of which is the immune responses. However, the function of Bombyx mori MoCoS in response to BmNPV remains unclear. In this study, BmMoCoS was found to be relatively highly expressed in embryonic development, gonads and the Malpighian tubules. In addition, the expression levels of BmMoCoS were significantly upregulated in three silkworm strains with different levels of resistance after virus infection, suggesting a close link between them. Furthermore, RNAi and overexpression studies showed that BmMoCoS was involved in resistance to BmNPV infection, and its antivirus effects were found to be related to the regulation of uric acid metabolism, which was uncovered by inosine‐ and febuxostat‐coupled RNAi and overexpression. Finally, the BmMoCoS‐mediated uric acid pathway was preliminarily confirmed to be a potential target to protect silkworms from BmNPV infection. Overall, this study provides new evidence for elucidating the molecular mechanism of silkworms in response to BmNPV infection and new strategies for the prevention of viral infections in sericulture.

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Section: Discussionmentioning

confidence: 99%