Influence of Aeromonas hydrophila and Pseudomonas fluorescens on motility, viability and morphometry of cryostored silver barb (Barbodes gonionotus) sperm

Boonthai, Traimat; Khaopong, Weerasith; Sangsong, Jumlong; Vuthiphandchai, Verapong; Nimrat, Subuntith

doi:10.1016/j.cryobiol.2016.08.008

Cited by 12 publications

(7 citation statements)

References 28 publications

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“…However, the true impact of bacterial infections on male fertility remains controversial. Pathogenic bacteria such as Escherichia coli ( Bussalleu et al, 2011 ), Enterobacteria ( Ubeda et al, 2013 ), Clostridium perpringens ( Sepulveda et al, 2013 ), Pseudomonas aeruginosa ( Sepúlveda et al, 2014 ), Enterobacter cloacae ( Prieto-Martinez et al, 2014 ), and Aeromonas hydrophila ( Boonthai et al, 2016 ) in semen were previously considered to be negatively associated with the sperm motility parameters, structural integrity, biochemical and physiological of sperm capacitation, even leading to suboptimal reproductive performance ( Maroto Martin et al, 2010 ). However, the correlation between bacteria in freshly ejaculated boar semen and sperm reproductive potential indexes is not clear.…”

Section: Discussionmentioning

confidence: 99%

“…However, the true impact of bacterial infections on male fertility remains controversial. Pathogenic bacteria such as Escherichia coli (Bussalleu et al, 2011), Enterobacteria (Ubeda et al, 2013), Clostridium perpringens (Sepulveda et al, 2013), Pseudomonas aeruginosa (Sepúlveda et al, 2014), Enterobacter cloacae (Prieto-Martinez et al, 2014), and Aeromonas hydrophila (Boonthai et al, 2016) in semen were previously considered to be negatively FIGURE 5 | KEGG analysis and function prediction of semen bacteria in winter and summer. (A) Environmental information processing pathway, (B) genetic information processing pathway, and (C) metabolism pathway.…”

Section: Discussionmentioning

confidence: 99%

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Genomic Sequencing Reveals the Diversity of Seminal Bacteria and Relationships to Reproductive Potential in Boar Sperm

et al. 2020

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A number of emerging studies suggest that pathogenic microorganisms in semen may cause a decline in the reproductive potential of spermatozoa, and the bacterial diversity and profile of ejaculated boar semen in different seasons are currently unknown. To explore the bacterial composition and changes in ejaculated boar semen from winter and summer, and the underlying mechanism of decline in sperm quality and fertility capacity in summer, 120 ejaculated semen samples were examined for bacterial communities using genomic sequencing technology, and the associations between microbial composition and sperm reproductive potential were investigated. The results showed that Proteobacteria (57.53%), Firmicutes (31.17%), Bacteroidetes (4.24%), and Actinobacteria (3.41%) are the dominant phyla in the ejaculated semen, and the dominant genera were Pseudomonas (34.41%) and Lactobacillus (19.93%), which belong to the phyla of Proteobacteria and Firmicutes, respectively. Interestingly, the higher diversity of bacteria in ejaculated semen of winter differs from that of summer semen, potentially due to seasonal changes related to changes in semen quality and sperm fertilizing capacity. Furthermore, the highly abundant Lactobacillus in winter samples were positively associated with sperm quality and reproductive performance obtained from sows inseminated with such semen samples, while in contrast, the highly abundant Pseudomonas in summer samples was negatively associated with sperm quality and reproductive potential. Additionally, our results strongly indicated that Lactobacillus is not only a potential probiotic for semen quality and fertility potential but also beneficial for restraining the negative influence of Pseudomonas . Overall, our findings significantly contribute to the current understanding of the phenotypes and etiology of male “summer infertility,” and may represent a frontier in male reproductive disorders and possible early prevention against pathogenic bacteria.

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Section: Discussionmentioning

confidence: 99%

“…However, the true impact of bacterial infections on male fertility remains controversial. Pathogenic bacteria such as Escherichia coli (Bussalleu et al, 2011), Enterobacteria (Ubeda et al, 2013), Clostridium perpringens (Sepulveda et al, 2013), Pseudomonas aeruginosa (Sepúlveda et al, 2014), Enterobacter cloacae (Prieto-Martinez et al, 2014), and Aeromonas hydrophila (Boonthai et al, 2016) in semen were previously considered to be negatively FIGURE 5 | KEGG analysis and function prediction of semen bacteria in winter and summer. (A) Environmental information processing pathway, (B) genetic information processing pathway, and (C) metabolism pathway.…”

Section: Discussionmentioning

confidence: 99%

Genomic Sequencing Reveals the Diversity of Seminal Bacteria and Relationships to Reproductive Potential in Boar Sperm

et al. 2020

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“…Horváth et al () proved that agglutination did not occur in ionic extenders in common carp; however, their efficiency was lower compared to the sugar based one. Furthermore, Boonthai et al () revealed that the presence of two pathogen bacteria ( A. hydrophila and P. fluorescens ) lead to a reduction in sperm motility after thawing and agglutination in another cyprinid fish, silver barb ( B. gonionotus ). According to the mentioned phenomenon, sugar can be a substrate for pathogen bacteria in the extender thereby its amount needs to be optimized.…”

Section: Discussionmentioning

confidence: 99%

“…Lower dilution ratios (related with sperm concentration) led to sperm agglutination following thawing compared to the higher dilution ratios (Yang et al, ). In silver barb ( Barbodes gonionotus ), the experiments revealed that the presence of pathogen bacteria ( Aeromonas hydrophila and Pseudomonas fluorescens ) cause reduced motility, viability, sperm agglutination and change in sperm morphometry (especially flagellum width) (Boonthai, Khaopong, Sangsong, Vuthiphandchai, & Nimrat, ). Sperm agglutination was observed more frequently in different cyprinids (Marinović et al, ).…”

Section: Introductionmentioning

confidence: 99%

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Várkonyi

Bokor

Molnár

et al. 2018

Reprod Domestic Animals

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Contents In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large‐scale (elevated volume of sperm) freezing method in a controlled‐rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54% ± 8%, Pike: 37% ± 5%), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled‐rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.

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“…Unlike the case for gut microbiota, there are few studies on the detection of bacteria in sperm/spermatophore in aquatic animals, even though bacteria are closely related with all life stages of marine organisms. 11 Boonthai et al 12 showed the influence of 2 inoculated freshwater fish pathogenic bacteria ( Aeromonas hydrophila and Pseudomonas fluorescens ) on silver barb sperm motility, sperm viability, and sperm morphology during cryopreservation. In addition, Flavobacterium psychrophilum , which is a cold-water disease, was transferred from male seminal fluid to the egg during fertilization.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

Koh

Nizam

Abduh

et al. 2019

Evol Bioinform Online

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Malaysian Mahseer ( Tor tambroides ) is considered as a good prospect for aquaculture in Malaysia. However, knowledge about Malaysian Mahseer-associated sperm microbiota is still limited, although some studies reported that sperm-related bacteria are a factor in the decline of sperm quality, as sperm may become the carrier of pathogenic bacteria to the egg. The goal of this study was to evaluate the sperm microbiota associated with Malaysian Mahseer from 3 different locations (Universiti Malaysia Terengganu [UMT], Ajil, and Pahang) using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting and to compare location differences by cluster analysis. Our results showed that the UMT sample had different sperm microbiota composition and a different trend in its relationship with sperm quality. Correlation analysis showed a relationship between bacterial diversity and sperm quality. Phylogenetic analysis indicated that sperm microbiota was composed of diverse phyla, including Proteobacteria, Firmicutes, and Actinobacteria. Interestingly, bacteria such as Salinisphaera sp., Pelomonas sp., and Staphylococcus spp. were detected in all the locations, suggesting that these bacteria are indigenous bacterial members of the Malaysian Mahseer sperm microbiota, although their function is still unclear.

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Influence of Aeromonas hydrophila and Pseudomonas fluorescens on motility, viability and morphometry of cryostored silver barb (Barbodes gonionotus) sperm

Cited by 12 publications

References 28 publications

Genomic Sequencing Reveals the Diversity of Seminal Bacteria and Relationships to Reproductive Potential in Boar Sperm

Genomic Sequencing Reveals the Diversity of Seminal Bacteria and Relationships to Reproductive Potential in Boar Sperm

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

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