Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer <i>Tor tambroides</i>

Koh, Ivan Chong Chu; Nizam, Bin Hassan Badrul; Abduh, Yazid Muhammad; Munafi, Ambok Bolong Abol; Iehata, Shumpei

doi:10.1177/1176934319850821

Cited by 3 publications

(4 citation statements)

References 45 publications

(51 reference statements)

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“…These discrepancies could be explained in part by the differences in microbial community composition across body sites (Minich et al, 2020;Clavere-Graciette et al, 2022). In the seminal fluid, the diverse microbiota was predominantly composed of 4 main phyla, which include Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria (Koh et al, 2019), also found in a study based on denaturing gradient gel electrophoresis.…”

Section: Discussionmentioning

confidence: 95%

“…Major taxonomic composition shifts were observed (ANCOM approaches) between wild and aquarium small-spotted catsharks in Serratia, Salinisphaera and Cutibacterium genera, being higher in managed care sharks, a factor associated with dysbiosis in other species (Garcia-Segura et al, 2022). Salinisphaera was ubiquitous in the Malaysian Mahseer sperm microbiota (Koh et al, 2019). Correspondingly, Cutibacterium has been identified in rays and skates (Goncalves et al, 2020;Mika et al, 2021;Garcia-Segura et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…However, the key elements connecting microbial communities in reproductive fluids and reproductive success are not defined (Koh et al, 2019;Rowe et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Unveiling microbiome signature in inner body fluids: comparison between wild and aquarium small-spotted catshark (Scyliorhinus canicula)

et al. 2023

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The microbiome is a crucial influencer in animal development, immune function and health, and it has complex and dynamic interactions with the environment, but little is known about the microbial signatures of inner body fluids. Recent evidence suggests that inner body fluids could be an indicator of the environmental interactions that fish experience. In the present study, we provide a comparative analysis of the microbial profile found in small-spotted catsharks’ blood plasma and seminal plasma and how microbial signatures vary between aquarium and wild animals. In the blood plasma microbiome, the habitat did not affect the α- and β-diversity, while in seminal plasma, both α- and β-diversity differed between both habitats. Proteobacteria are the main bacteria dominated independently the inner body fluid and habitats. No core microbiome was identified at the genus level, with only Pseudomonas and Cloacibacterium present in both inner body fluids and habitats. Of the 14 genera identified in blood plasma, only four were shared between habitats (making up 45.17% and 51.03% of relative abundance for wild and aquarium, respectively). Similarly, of the 100 genera identified in seminal plasma, only 41 were shared between habitats (84.79% and 64.83%, respectively). Moreover, in the seminal plasma, using ANCOM approaches, Serratia, Salinisphaera and Cutibacterium were found significantly enriched in aquarium animals. None potentially pathogenic bacteria were identified in the blood samples, while Coxiella, Prevotella, Coprococcus, Haemophilus and Phocoenobacter were potentially pathogenic bacteria identified in the seminal plasma samples. In summary, this study provides evidence of a circulating blood and seminal plasma microbiome in healthy small-spotted catsharks. Furthermore, dynamic changes were observed in the microbiome of these inner body fluids, which differed between the aquarium and wild habitats.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Unveiling microbiome signature in inner body fluids: comparison between wild and aquarium small-spotted catshark (Scyliorhinus canicula)

et al. 2023

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“…and Bacteroides sp. supplementation improves the sperm quality of Tor tambroides [191]. Application of Lab.…”

Section: Malementioning

confidence: 99%

Epigenetics and Probiotics Application toward the Modulation of Fish Reproductive Performance

Sumon

Molla

Hakeem

et al. 2022

Fishes

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Fish represent an excellent source of animal protein as well as a biomedical research model as a result of their evolutionary relatedness and similarity with the human genome. Commercial and ornamental fish culture has achieved popularity, but reproductive dysfunctions act as a limiting factor for quality fry production, interfering with the sustainability of the aquaculture industry. Fish reproduction is crucial for any species’ existence, and reproductive performance can potentially be improved through applications of epigenetics and probiotics. Epigenetics is a highly sensitive molecular approach that includes chromatin structure and function alteration, DNA methylation, and modification of non-coding RNA molecules for the transfer of desired information from parents to offspring. DNA methyltransferase improves reproductive cyp11a1, esr2b, and figla gene expression and feminizes zebrafish (Danio rerio). Moreover, epigenetics also contributes to genome stability, environmental plasticity, and embryonic development. However, methylation of specific genes can negatively affect sperm quality, resulting in poor fertilization. Probiotic administration is able to induce responsiveness of incompetent follicles to maturation-inducing hormones and can change oocyte chemical composition during vitellogenic development. The positive role of probiotics on testicular cells is validated by upregulating the transcription levels of leptin, bdnf, and dmrt1 genes facilitating the spermatogenesis. This review not only discusses the effects and mechanism of epigenetics and probiotics for improving fish reproduction, but also presents an overview of the causal factors and current techniques used to eradicate dysfunction. Moreover, key genes and hormones related to fish reproduction along with research gaps and future prospects are also considered. This review provides an overview of necessary information for students, scientists, researchers, and breeders to resolve fish reproduction-related problems to ensure profitable and sustainable aquaculture.

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A Review on the Emerging Asian Aquaculture Fish, the Malaysian Mahseer (Tor tambroides): Current Status and the Way Forward

et al. 2021

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The Malaysian mahseer (Tor tambroides) is a sport, ornamental and food all-in-one treasured aquaculture fish member of the Cyprinidae family. However, this freshwater riverine species is currently under the threats of habitat degradation and overfishing, apart from its poorly documented taxonomy. Despite its socio-economic significance, taxonomy confusion and conservation inadequacy, the knowledge gaps in some fields like the genomic landscape and biology are yet to be filled. In this comprehensive review, we presented an overview of all the past and current T. tambroides related research fields, namely morphology, population genetics, growth environment, reproduction, diet and nutrition, metagenome, mitogenome as well as conservation. Furthermore, we have included some future perspectives and recommendations for the advancement of the respective fields. It is anticipated that this review would be of great benefit to those planning to consolidate resources and work in tandem to pace the conservation and fish farming industry improvement endeavours.

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Molecular Characterization of Microbiota Associated With Sperm of Malaysian Mahseer Tor tambroides

Cited by 3 publications

References 45 publications

Unveiling microbiome signature in inner body fluids: comparison between wild and aquarium small-spotted catshark (Scyliorhinus canicula)

Unveiling microbiome signature in inner body fluids: comparison between wild and aquarium small-spotted catshark (Scyliorhinus canicula)

Epigenetics and Probiotics Application toward the Modulation of Fish Reproductive Performance

A Review on the Emerging Asian Aquaculture Fish, the Malaysian Mahseer (Tor tambroides): Current Status and the Way Forward

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