Influence of age on ergosterol content in mycelium of Aspergillus ochraceus

Torres, Mercè; Viladrich, R.; Sanchís, V.; Canela, Ramón

doi:10.1111/j.1472-765x.1992.tb00713.x

Cited by 20 publications

(9 citation statements)

References 7 publications

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Section: Discussionmentioning

confidence: 89%

“…The differences between the formation of this sterol and growth of mould involved the fact that in the stationary phase the reduction in the level of ergosterol in the mycelium occurred earlier than the reduction in the quantity of dry mass. Torres et al (1992) also noted in his investigations that ergosterol content varied with the age of the culture differently than mycelium biomass. Mille-Lindblom et al (2004) confirmed in their investigations a significant 34% degradation in ergosterol in mycelium after the death of fungi, the degradation being greater in case of additional action of light.…”

Section: Discussionmentioning

confidence: 89%

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Mathematical models of mycelium growth and ergosterol synthesis in stationary mould culture

Gutarowska

Żakowska

2009

Letters in Applied Microbiology

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Aims: To develop mathematical models for mycelium growth and ergosterol formation in conditions of periodic stationary culture; to verify possibilities of applying a model describing the relationship between ergosterol content and mycelium quantity. Methods and Results: The mould growth and ergosterol formation models covering all phases of mould growth are described using a modified logistic equation with the addition of an exponential function. The correlation between ergosterol and mycelium biomass depended on the growth phase of mould. Meaningful relation was obtained for initial two phases, when both parameters were growing equally. The quadratic function for estimation of the biomass based on ergosterol content was formulated. The error resulting from the application of this function in initial phases of moulds growth was small at 5–7%, in the following phases it was at 11–31%. Conclusions: Mycelium biomass can be precisely determined basing on the ergosterol content, when we know the moulds growth phase. In natural environments, without the information about growth phases, it will be possible, but with the higher error. Significance and Impact of the Study: Presented model based on the ergosterol content making possible to estimate the quantity of mycelium in moulds cultures and natural environments.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 89%

Mathematical models of mycelium growth and ergosterol synthesis in stationary mould culture

Gutarowska

Żakowska

2009

Letters in Applied Microbiology

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“…At nitrogen concentrations of 0.5 -0.9 g/1 of different amino acids, the production of lanosterol (R,:0.5) was inhibited, but at 0. [25].…”

Section: Triterpenesmentioning

confidence: 99%

The effects of some amino acids on growth and lipid production in Inonotus obliquus grown in vitro

Kahlos

1994

Acta Biotechnologica

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The effect of the distribution of free and esterified fatty acids, including triterpenes and sterols, on the mycelial growth of the basidiomycete Inonotus obkquus (Pers. ex Fr.) Pilat in malt extract and solid supplemented mineral media was investigated. The amino acids DL-alanine, DL-aspartic acid, L-glutamic acid, L-leucine, L-methionine, and L-senne were added to the cultures at nitrogen concentrations of 0.1, 0.5 and 0.9 g/l, respectively. Differences in the average mycelial growth were distinct at concentrations of 0.5 g/l and 0.9 g/l. L-Methionine had the highest inhibitory effect on growth. At all concentrations, the amino acids reduced the production of lanosterol. The other identified triterpenes were inotodiol, 3/?,hydroxy-lanosta-8,24-dien-21-al, 3/?,21-dihydroxy-lanosta-8,24-diene, trametenolic acid, and ergosterol peroxide. The main fatty acids were either C 16:O or C 18 : 2 both free or in glycerides.

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“…Mille-Lindblom et al ( 2004 ) showed that it underwent significant degradation (43 %) under the action of light. Also ergosterol synthesis has been found to be significantly influenced by phase of moulds growth, ageing, and environmental parameters (media, pH, temperature) (Torres et al 1992 ; Gutarowska and Żakowska 2009 ). Studies have shown that on the death of mould the ergosterol content may fall by as much as 34 % (Mille-Lindblom et al 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of ergosterol content in the air of various environments

2014

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The aim of the study was to compare the content of ergosterol in different microorganisms (bacteria, yeasts and moulds) isolated from the air as well as in six species of moulds in their different morphological forms-live mycelium, dead mycelium, and spores. Evaluation of the level of mould contamination of the air in various places using culture method and ergosterol determination was also performed. The analysis of ergosterol was carried out by gas chromatography equipped with flame ionisation detector. For evaluation of the results, analysis of variance and multiple comparison test were used. The quantity of ergosterol in the spores of various species of mould was in the range 1.9-9.4 pg/spore. The presence of yeasts and bacteria in the air does not significantly affect ergosterol concentration, in view of the low content of that sterol in their cells (max. 0.009 pg/cell for bacteria and 0.39 pg/cell for yeast). An ergosterol concentration above 1 ng per m 3 can be considered an indicator of excessive mould contamination of indoor air. Based on determination of ergosterol in the air of mouldy rooms the result obtained may be compared with the culture method, due to the 1,000 times higher concentration of ergosterol in the mycelium compared with spores. However, in the analysis of outdoor air, in view of the presence of mould mainly in the form of spores and the degradation of ergosterol by UV radiation, analysis of that compound may indicate a lower level of contamination compared with the culture method.

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Influence of age on ergosterol content in mycelium of Aspergillus ochraceus

Cited by 20 publications

References 7 publications

Mathematical models of mycelium growth and ergosterol synthesis in stationary mould culture

Mathematical models of mycelium growth and ergosterol synthesis in stationary mould culture

The effects of some amino acids on growth and lipid production in Inonotus obliquus grown in vitro

Evaluation of ergosterol content in the air of various environments

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