Influence of antioxidant rich fresh vegetable juices on starch induced postprandial hyperglycemia in rats

Abstract

This research analyzed the major chemical components and multiple antioxidant activities present in the fresh juice of eight vegetables, and studied their influence on starch induced postprandial glycemia in rats. A SDS-PAGE based protein fingerprint of each vegetable juice was also prepared. The yields of juice, chemical components like total proteins, total polyphenols, total flavonoids, total anthocyanins and free radicals like the ABTS˙(+) cation, DPPH, H(2)O(2), scavenging activities and reducing properties for NBT and FeCl(3) showed wide variations. Vegetable juice from brinjal ranked first in displaying total antioxidant capacity. Pretreatment of rats with vegetable juices moderated starch induced postprandial glycemia. The fresh juice from the vegetables ridge gourd, bottle gourd, ash gourd and chayote significantly mitigated postprandial hyperglycemic excursion. Total polyphenol concentrations present in vegetable juices positively influenced ABTS˙(+) scavenging activity and total antioxidant capacity. However, NBT reducing activity of juices was positively affected by total protein concentration. Contrarily, however, high polyphenol content in vegetable juice was observed to adversely affect the postprandial antihyperglycemic activity of vegetable juices. This is the first report exploring antihyperglycemic activity in these vegetable juices and highlights the possible adverse influence of high polyphenol content on the antihyperglycemic activity of the vegetable juices.

Pigeon pea seed husks as potent natural resource of anti-oxidant and anti-hyperglycaemic activity

“…[29] Highly processed, calorie dense, nutrient depleted diet leads to exaggerated postprandial spikes in blood glucose and induces immediate oxidative stress. [15,17] Postprandial hyperglycaemia is a prominent and early defect ensuing T2DM, [30] an independent predictor of development of future cardiovascular events, [31] atherosclerosis and development of oxidative stress. [32] Postprandial state is also referred to as pro-oxidative state, which involves active oxidative metabolism and generation of free radicals.…”

“…FeCl 3 reducing activity [15] was modified to suit 96 well plates reading. In Eppendorf tubes, 100 µL of various dilutions of sample extract were mixed with 100 µL of phosphate buffer and incubated with 100 µL of 1% potassium ferricyanide at 50°C for 20 min.…”

“…Recently, we have explored and identified anti-oxidant and anti-hyperglycaemic activities in number of Indian food materials. [15][16][17][18][19] In the present study, we report analysis of phytochemical components, anti-oxidant properties and anti-hyperglycaemic activity in pigeon pea seed husk.…”

Vegetables′ juice influences polyol pathway by multiple mechanisms in favour of reducing development of oxidative stress and resultant diabetic complications

“…[8] Furthermore, eating vegetables before carbohydrate rich meals have been found to improve postprandial glycemic excursion in clinical settings. [9] Recently, certain vegetables’ juice has been identified to display antihyperglycemic activities[1011] through various mechanisms. [1112] Vegetables’ juice has also been observed to reduce development of hyperglycemia induced oxidative stress and imbalance in physiological functions.…”

Quality Changes of Fresh Vegetable and Fruit Juice by Various Juicers

“…또한 과일주스에 포함된 성분 가운데 pycnogenol이 농축된 주스 (농도 0.5 g/L)를 이용하여 대장암 세포주 (Caco-2)의 증식의 억제 효과가 있다는 연구가 발표 되기도 하였다 [4]. 이 항암 효과는 여러 지표 가운데 항산화 활성과 밀접하게 관련이 있는 것으로 나타났으며, 항산화 활 성이 풍부한 과일주스가 전분에 의해 유도된 후기 고혈당증 (starch induced postprandial hyperglycemia)의 예방과 치료에 효과적이라는 것이 동물 (rat) 실험으로 증명되기도 하였다 [5]. 채소와 과일주스의 이러한 효과는 이 주스에 포함된 성 분에 의해 유발된다.…”