Influence of antipyretic drugs on the labeling of blood elements with Technetium-99m

Abstract

Acetaminophen (AAP), acetylsalicylic acid (ASA) and dipyrone (DIP) are antipyretic and analgesics drugs that have wide use in health sciences. Some drugs can modify the labeling of blood elements with technetium-99m (99mTc). This work has evaluated the effect of AAP, ASA and DIP on the labeling of the blood elements with 99mTc. Blood was incubated with different concentrations of the drugs before the 99mTc-labeled process. Plasma (P), blood cells (BC), insoluble (IF-P, IF-BC) and soluble (SF-P, SF-BC) fractions were separated and percentage of radioactivity (%ATI) in each fraction was determined. Data have shown that the antipyretic drugs used in this study did not significantly modify the fixation of 99mTc on the blood elements when the experiments were carried out with the doses usually used in human beings. Although the experiments were carried out with rats, it is possible to suggest that AAP, ASA or DIP should not interfere with the procedures in nuclear medicine involving the labeling of blood elements with 99mTc.

Assessment of the effect of Bacopa monnieri (L.) Wettst. extract on the labeling of blood elements with technetium-99m and on the morphology of red blood cells

“…There are evidences that various medicinal plants can affect either radiolabeling or biodistribution of red cells in the context of the nuclear medicine clinic, and a number of workers have turned their attention to in vitro testing of the drug (synthetic/natural) with 99mTC labeled blood cells (Hladik et al, 1982(Hladik et al, , 1987Sampson, 1996;Frydman et al, 2004;Fonseca et al, 2005). Furthermore, the developing of model that permits to evaluate pharmacologic properties of natural products is worthwhile.…”

“…It is described that natural and synthetic products can alter the labeling of blood constituents with 99mTc (Frydman et al, 2004;Valenca et al, 2005;Fonseca et al, 2005). The labeling of blood constituents could decrease due to the action of drugs (natural and synthetic) in (1) binding at the same sites on the blood constituents, (2) direct inhibition (chelating action) of the stannous (Sn +2 ) and pertechnetate ions (99mTcO -), (3) direct oxidation or generation of free radicals that could oxidize the stannous ion, (4) antioxidant action impeding or decreasing the stannous ion oxidation, and (5) alteration of the plasma membrane structure or modifying the transport systems of stannous and pertechnetate ions into cells.…”

“…Authors have reported that many natural or synthetic drugs can alter the labeling of blood elements with 99mTc (Haldik et al, 1982(Haldik et al, , 1987Srivastava & Straub, 1992;Gutfilen et al, 1993;Santosh et al, 1995;Oliveira et al, 1997;Oliveira et al, 2003a;2003b;Frydman et al, 2004;Moreno et al, 2004;Fonseca et al, 2005). There are some studies about the effect of the medicinal plants (Thuya occidentalis, Nicotiana tabacum, Peumus boldus, Maytenus ilicifolia, Paullinia cupana, cauliflower) on the labeling of RBC with 99mTc (Hladik et al, 1987;Hesslewood & Leung, 1994;Sampson, 1996;Oliveria et al,1997Oliveria et al, , 2002Oliveria et al, , 2003aVidal et al, 1998;Dire et al, 2003).…”

Evaluation of the effect of an extract of sabugueiro (Sambucus australis) on the labeling of blood constituents with technetium-99m

“…Moreover, investigations in basic research have permitted to understand better some properties related with the medicinal plants (Fonseca et al, 2005;Bernardo-Filho et al, 2005). Sambucus australis, known as sabugueiro in Brazil, is used in folk medicine as diuretic, to treat inflammation, burn, pain and rheumatologic disorders (Guarrera et al, 2005;Jorge et al, 1999).…”

Acetylsalicylic acid and labeling of blood constituents with technetium-99m

“…Red blood cells labeled with technetium-99m (99mTc-RBC) have been used in nuclear medicine in a number of evaluation procedures for the gastrointestinal bleeding sites (Wong et al, 2004;Zaman et al, 2004;Olds et al, 2005), peripheral arterial blood flow (Harel et al, 2005), hepatic hemangiomas (Artiko et al, 2004;Verdu et al, 2005), renal carcinoma (Cortes et al, 2003) and splenic reticuloendothelial system (Jin et al, 2004;Slart et al, 2004). Data have shown that some drugs can modify the labeling of blood constituents, such as the RBC, with 99mTc and should alter the results obtained in the daily routine procedures in nuclear medical laboratories (Hladik III et al, 1987;Capriles et al, 2002;de Oliveira et al, 2002;Frydman et al, 2004;Fonseca et al, 2005). The aim of this work was to investigate the in vitro and in vivo effects of acetylsalicylic acid on the labeling of blood constituents with 99mTc.…”