The influence of complex aging processes in water-miscible metalworking fluids on process performance is of high relevance for the metalworking industry. Because of the highly dynamic interactions in the complex "metalworking fluid" ecosystem, a distinct correlation between the aging process and the performance of the fluid in metalworking processes is hardly possible. Consequences of the aging process on physical, chemical, and biological properties of the fluid include aspects such as the decrease of the pH value, the increase of the droplet size in emulsions, the presence of bacterial cells, or the modification of the metalworking fluid composition. In the presented work, the influences of these aging aspects on the lubricity of metalworking fluids were investigated individually. A test series has been carried out, which was planned with a design of experiments method, to investigate interactions between the aging aspects regarding lubricity. In addition, the results enabled the development of an empirical regression model, which allowed an integrated description of the influence of the relevant aging aspects.Lubricants 2019, 7, 94 2 of 12 system, and the temperature is constantly within a convenient sector. The microorganisms can utilize the wide range of chemical compounds in metalworking fluids almost entirely to cover their demand of carbon and energy [5]. An intense maintenance of the cooling lubricant is crucial for a long service life, whereby particular attention is given to the bacterial load. The colonization of metalworking fluids by microorganisms such as bacteria or fungi has been investigated since the 1940s and is still the subject of various research activities [6][7][8][9][10][11]. When the fluid has been colonized, microbial growth in metalworking fluids can neither be completely avoided nor is the initial state of the fluid composition recoverable. Within a few weeks, the microbial load of metalworking fluids can reach a level of 10 7 (or more) colony-forming units per milliliter [8,10]. The standard method of avoiding bacterial load is the addition of biocidal products such as bactericides and fungicides [9]. The treatment with ultrasound, ozone, or UV radiation are further strategies [11]. All those methods reveal specific disadvantages, such as negative influence on emulsion properties, localized or selective impact, or they are harmful to health and the environment [1].Simultaneous to a decreasing number of additives over time due to the metabolic activity of microorganisms, compounds of microbial origin are released continuously in the metalworking fluid, such as low-molecular metabolites, surface-active agents, or extracellular polymeric substances (EPSs). The changes taking place in the cooling lubricant are shown schematically in Figure 1. Other consequences of bacterial colonization are decreasing pH values, increasing droplet sizes [8], or mechanical problems in the metalworking fluid cycle due to clogging of filters, pumps, and so on, by biofilms [7]. Further changes in the metalw...