Influence of Bioreceptor Layer Structure on Myelin Basic Protein Detection using Organic Field Effect Transistor‐Based Biosensors

Abstract

Organic field-effect transistor (OFET)-based biosensors with antibody receptors are widely considered for protein antigen detection. To the authors' knowledge, there are no comparative evaluations to date of different choices for the matrix polymer to which the antibodies are attached. Herein, multiple acrylic copolymers are studied as receptor layers with myelin basic protein and its corresponding antibody as an archetypal antibodyantigen pair. Stability against multiple washing steps and the capacity for immobilizing antibodies on polymers on device surfaces with the help of fluorescein isothiocyanate-labeled antibodies are compared. Electronic detection and selectivity are also observed. The conclusions provide guidance on the selection of bioreceptor material for increasing sensitivity and process stability of OFET biosensors.

Impedance spectroscopic detection of binding and reactions in acid-labile dielectric polymers for biosensor applications

“…A separate film was cast containing the same polymer blend without the EDC-NHS amine crosslinker molecules. Films were washed and incubated with a commercially-available FITC-tagged antibody to GFAP (as GFAP and PBP2a are both proteins with similar charge and size) 34 . FITC-tagged monoclonal antibodies corresponding to the same PBP2a epitope (amino acids 19–348) were not readily commercially available, but the anti-GFAP-antibody used in this experiment was also IgG1 from murine source, meaning the bulk of the antibody (including the heavy chain and amine group where our crosslinking chemistry of interest is taking place) was nearly identical except for the necessary fluorescent tag, making this product an appropriate substitute to examine antibody-surface interaction.…”

Recent Advances in Flexible Field‐Effect Transistors toward Wearable Sensors

“…[ 196 ] With respect to FET‐based biochemical sensors, biochemical substances are absorbed on the active layer, which is usually a biochemical recognition semiconductor, and can modify the carrier density and the doping level of the receptor. [ 197,198 ] By capturing data of specific molecules from the human body, F‐FET biochemical sensors are able to retrieve more insightful health information. In conventional clinical or laboratory scales, health examinations primarily depend on blood analysis.…”

“…[196] With respect to FET-based biochemical sensors, biochemical substances are absorbed on the active layer, which is usually a biochemical recognition semiconductor, and can modify the carrier density and the doping level of the receptor. [197,198] By capturing data [71] Copyright 2014, Springer Nature Limited. d) Conductance response of P3HT-based chemical sensors toward acetone and toluene detection, carrier gas 1 L min À1 N2.…”

Microfluidic devices for the detection of viruses: aspects of emergency fabrication during the COVID-19 pandemic and other outbreaks

“…In these devices, the interactions between the binding elements immobilized on the surface and the target particles in a solution induce electrostatic perturbations that alter the voltage threshold of the transistor [ 114 ]. The curves of the characteristic transfer function show a shift upon antigen-antibody binding in comparison to non-specific antigens [ 105 , 106 , 116 ] ( figure 5 b ). FET-based sensors were described to detect the Ebola virus through the immobilization of Anti-EBOV antibodies on the surface of the device and by monitoring the electric current [ 105 ].…”