Influence of “Brovadez-plus” disinfectant in different concentrations on Eimeria oocysts in calves

Abstract

The purpose of work was to determine the effect of different concentrations of “Brovadez-plus” disinfectant solution on the sporulation process of mixed culture of eimerias oocysts of calves. Disinvasive activity of the drug “Brovadez-plus” was determined at concentrations of 1.5, 2, 3 and 3.5% for exposures of 3, 5 and 8 hours by irrigation of oocysts. The drug “Brovadez-plus’ at 2% concentration for 3 hours exposure showed an eimeriostatic effect at the level of 29.9 ± 1.4%. At 5 hours exposure, sporogonia did not undergo 51.1 ± 0.5% oocysts, and at 8 hours exposure 64.4 ± 2.2% eimerias oocysts of calves. When using 3% concentration of “Brovadez-plus” solution for 3 hours, sporulation process did not take place in 57.8 ± 0.6% eimerias oocysts, 2.7 ± 0.9% oocysts completed sporulation and 19.6 ± 0.9 % — the sporulation was delayed. The best results were obtained with the use of a 3% solution at an exposure of 5 and 8 hours. The sporogony process did not occur in 96.6 ± 1.2% and 98.2 ± 0.8% of oocysts of eimerias. When using “Brovadez-plus” disinfectant at a concentration of 3.5% at exposure for 3 hours sporogonia did not pass in 78.5 ± 1.1% of eimerias oocysts, at exposure for 5 hours in 96.8 ± 2.1% of eimerias oocysts at exposure 8 hours in 99.1 ± 2.4% of eimerias oocysts of calves. Thus, experimental studies have shown that the drug “Brovadez-plus”, when used in 3% and 3.5% concentrations, has a high level of disinvasive properties relative to eimerias oocysts of calves. Experimental studies have established that the detrimental effect on eimerias oocysts is directly proportional to the duration of their contact and the concentration of the working solution of the studied drug. When using 3% and 3.5% concentrations of “Brovades-plus” disinfectant at an exposure of 5 and 8 hours in 99.1 ± 2.4% of eimerias oocysts of calves, the process of sporogony did not occur