Influence of Ca2+ and 6-benzyladenine on chestnut (Castanea sativa Mill.) in vitro shoot-tip necrosis

Piagnani, C.; Zocchi, Graziano; Mignani, I.

doi:10.1016/0168-9452(96)04423-8

Cited by 24 publications

(28 citation statements)

References 12 publications

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“…There are, however, different opinions as to how they affect the problem which can be categorized into three groups. Some suggest that the cause is due to eliminating or reducing the concentration of CKs in the media (Kataeva et al 1991;Piagnani et al 1996). On the other hand there are reports to the contrary.…”

Section: Introductionmentioning

confidence: 87%

Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

et al. 2011

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Changes in cytokinin (CK) profiles and their physiological implications in micropropagated Harpagophytum procumbens [(Burch.) DC. ex Meisn.] tissues in relation to shoot-tip necrosis (STN) and CK treatments were studied. Total CK content was quantified in benzyladenine (BA)-treated necrotic and normal plantlets and in plantlets treated with the CKs BA, meta-topolin (mT) and meta-topolin riboside (mTR) with and without the auxin indole-3-acetic acid (IAA). Generally necrotic shoots yielded more total CK compared to normal shoots. Cytokinin accumulation was higher at the basal section (basal [ middle [ top). Further analysis of the CKs based on structural and functional forms revealed excessive accumulation of 9-glucosides (deactivation products-toxic metabolites) and limited amounts of O-glucosides (storage forms-re-utilizable) in necrotic and BA-treated shoots compared to normal and topolin-treated cultures. The addition of IAA enhanced the formation of 9-glucosides in BA-treated cultures but reduced it in topolin-treated cultures. The symptom of STN could therefore be attributed to conversion of active cytokinins to other forms such as 9-glucosides which are neither active nor reversibly sequestrated to active forms. Literature shows that metabolites like 9-glucosides of BA have a detrimental effect in plant tissue culture.

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Section: Introductionmentioning

confidence: 87%

Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

et al. 2011

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“…Some report that the use of higher concentrations of Ca alleviates or controls the STN (Dyson and Digby 1975, Vieitez et al 1989, Singha et al 1990, Barghchi and Alderson 1996, Piagnani et al 1996, Wang and Van Staden 2001, Chang and Miller 2005, Martin et al 2007). In contrast, other reports show that high Ca concentrations in the culture media significantly increase the percentage of STN (Grigoriadou et al 2000).…”

Section: Resultsmentioning

confidence: 99%

“…The symptoms of nutrient deficiency of less mobile elements such as calcium (Ca) and boron (B) (Raven 1977) first appear in the meristematic regions and young leaves whereas symptoms of excessive amounts of these minerals are first observed on the older leaves (Barghchi and Alderson 1996). The problem of non-pathogenic dieback or shoot necrosis in vitro has been associated with complex set of factors such as salt formulation, growth regulators, use of additives like charcoal to medium, sugar source, frequency of subculturing affecting it, physiological disorders associated with rooting, sulphur content and NH4/NO3 ratio and pH fluctuations (Vieitez et al 1989, De Block 1990, Kataeva et al 1991, Lakshmi and Raghava 1993, Mackay et al 1995, Piagnani et al 1996, Grigoriadou et al 2000, Seling et al 2000, Wang and Van Staden 2001and Jain et al 2009). …”

Section: Introductionmentioning

confidence: 99%

In vitro Shoot Regeneration and Control of Shoot Tip Necrosis in Tissue Cultures of Soymida febrifuga (Roxb.) A. Juss.

Chiruvella

Mohammed

Dampuri

et al. 2012

Plant Tissue Cult. & Biotech.

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Soymida febrifuga (Roxb.) A. Juss., (Meliaceae) an indigenous lofty deciduous tree, endemic to India, has extensive pharmacological activity. Continuous destruction of plants due to environmental and geopolitical instability has posed a major threat to endemic tree species. In vitro propagation techniques are also problematic due to high incidence of shoot tip necrosis (STN). The effect of various culture conditions on regeneration and STN of Soymida febrifuga were investigated. Nodal segments from field grown plants were used as explants and cultured on MS supplemented with different concentrations of BA, KN, NAA and IAA. The synergistic effect of BA (2 mg/l) and NAA (0.2 mg/l) induced a mean of 6.3 ± 0.09 shoots from the nodal explants with a frequency of 80.4%. Frequent subculturing of nodal explants from in vitro derived shoots increases the number of multiple shoots, but the regenerated shoots exhibited the symptoms of STN. Various factors such as strength of the media, different carbon sources activated charcoal and different calcium sources have been investigated for reducing the incidence of shoot necrosis. STN can easily be recovered by increasing the levels of calcium. Transfer of shoots showing early signs of necrosis to half strength MS supplemented with CN (556 mg/l), CP (1.0 mg/l), AC (20 mg/l) and fructose (100 mg/l) facilitated recovery of more than 98% of the shoots. Rooted plantlets produced, using the optimized protocol, were acclimatized successfully.

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“…The absorption and the translocation of Ca through the plant tissues are dependent of the mass flow and it is limited by the low transpiration of the explants cultivated in the flasks under high relative humidity, reducing the transport of ions of low mobility as the calcium (Sha et al 1985). This symptom of shoot tip necrosis caused by calcium deficiency occurs in both ex vitro and in tissue culture (Sha et al 1985;Piagnani et al 1996). Characteristic symptoms of calcium deficiency include necrosis of young meristematic regions, such as the tips of roots or young leaves, where cell division and wall formation are most rapid.…”

Section: Culture Conditions and Statistical Analysismentioning

confidence: 99%

“…In this context, one of the strategies to overcome this problem is the modification of the composition of the culture medium. Ca 2+ concentration in the culture medium has been associated with the shoot-tip necrosis, which decreases when the calcium content increases in the medium for in vitro culture of Castanea mollissima, C. dentata and C. sativa (Qiguang et al 1985;Piagnani et al 1996). Calcium is an integral component in the cell wall and plays an important role in maintaining membrane integrity (Marschner 1995;Machado et al 2010).…”

Section: Lavandula Angustifolia Mill (Syn L Officinalismentioning

confidence: 99%

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

Machado

Silva

Biasi

et al. 2014

Braz. arch. biol. technol.

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Influence of Ca2+ and 6-benzyladenine on chestnut (Castanea sativa Mill.) in vitro shoot-tip necrosis

Cited by 24 publications

References 12 publications

Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

In vitro Shoot Regeneration and Control of Shoot Tip Necrosis in Tissue Cultures of Soymida febrifuga (Roxb.) A. Juss.

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

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