Influence of cadmium ions on the antioxidant status and lipid peroxidation in mouse liver: protective effects of zinc and selenite ions

Bernotienė, Rasa; Ivanovienė, Laima; Sadauskienė, Ilona; Liekis, Arūnas; Ivanov, Leonid

doi:10.5414/te0x1229

Cited by 8 publications

(17 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, Zn may prevent the effects of Cd because it prevents Cd action on the oxidative phosphorylation in the liver mitochondria as already observed by Friberg et al (1974). Bernotiene et al (2012) evaluated the influence of Zn and Se on the antioxidative systems of mice affected by Cd. They applied intraperitoneal injections of CdCl 2 , ZnSO 4 , and Na 2 SeO 3 solutions to white outbred laboratory mice and observed that Zn and Se prevented liver lipids against Cd-induced peroxidation and did not suppress effects of Cd on MT contents in mice liver.…”

Section: Discussionmentioning

confidence: 99%

Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model

Zídková¹,

Melčová²,

Bartosova³

et al. 2014

CZECH J ANIM SCI

View full text Add to dashboard Cite

ABSTRACT:The response of different strains of laboratory rats (Rattus norvegicus L.) on both acute (via intraperitoneal injection) and chronic (via drinking water and/or diet) cadmium intoxication was investigated in the model study. The rat strains Long Evans (LE), Spontaneously hypertensive rat (SHR), and Brown Norway (BN) were tested and compared, and total Cd levels and metallothionein (MT) concentrations were determined in the liver of experimental animals. The liver MT concentrations were determined by using adsorptive chronopotentiometry and modified Brdička reaction and were significantly correlated (r = 0.965) with the total liver Cd content. Moreover, the Cd application resulted in increasing zinc liver contents confirming intensive MT synthesis in the rat liver. In the blood plasma, specific enzymatic activity of glutathione reductase (GR) and glutathione-S-transferase (GST) was determined suggesting increasing activity of GR with the amount of applied Cd for all three strains, whereas ambiguous results have been found for the activity of GST. Therefore, MT concentrations seemed to be more sensitive indicators of the Cd intoxication compared to the assessment of the specific enzymatic activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model

Zídková¹,

Melčová²,

Bartosova³

et al. 2014

CZECH J ANIM SCI

View full text Add to dashboard Cite

show abstract

“…Numerous data shows that many of the toxic effects of Cd result from interactions at different stages of the absorption, distri bution, and excretion with essential elements such as zinc (Zn) and selenium (Se) [11,12], Zn is an essential trace element for a num ber of cellular processes: DNA synthesis, transcription, and translation. This metal is essential for over 300 enzymes and nearly 2,000 transcription factors [13], Zn is consid ered as an antioxidant because it protects the cell against oxidative damages [12,14], but in excess it can be toxic [15].…”

Section: Introductionmentioning

confidence: 99%

“…This metal is essential for over 300 enzymes and nearly 2,000 transcription factors [13], Zn is consid ered as an antioxidant because it protects the cell against oxidative damages [12,14], but in excess it can be toxic [15]. Suppression of both RNA-related and translation path ways is a mechanism underlying deleterious effects of high concentrations of Zn2+ [16], Se is essential for the synthesis of selenopro teins [17] and acts as antioxidant [18].…”

Section: Introductionmentioning

confidence: 99%

The comparison of the protective effect of zinc and selenite ions against cadmium induced changes of protein synthesis in mice organs

Stanevičienė¹,

Bernotienė²,

Sadauskienė³

2014

Self Cite

View full text Add to dashboard Cite

“…The hepatic GSH depletion may also be associated with the protection against endogenous oxygen radi cals and the synthesis of MT. Although MT synthesis requires cysteine, which may be derived from the breakdown of GSH, there is no evidence that GSH itself has an additional function in MT formation (Bernotiene et al, 2012). GSH depletion leads to cell death, and may be the ultimate factor determining vulnerability to oxidant attack (El-Marghy et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Influence of cadmium ions on the reduced glutathione and lipid peroxidation in the liver and red blood cells of mice

Čijauskaitė¹,

Bernotienė²,

Ivanov³

2014

Biologija

Self Cite

View full text Add to dashboard Cite

The present investigation was undertaken to evaluate the influence of Cd on the reduced glutathione (GSH) and lipid peroxidation in liver and in red blood cells of mice. Ex periments were done on outbred white laboratory mice using intraperitoneal injections of CdCl 2 solution (14 μmol Cd/kg body mass). The exposure-time was 2 h, 8 h, 24 h and 14 days. GSH was measured by reaction with 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) to give a compound that absorbs 412 nm light wavelength. Lipid peroxides were es tima ted by measuring thiobarbituric-acid-reactive subs tan ces and were expressed as malondialdehyde (MDA). Our results in dicate that at the 8 th h the content of GSH in mice liver sig nificantly increased by 35%, meanwhile in red blood cells the content of GSH exceeded the control level at the 2nd h, 8 th h and 24 th h by 19%, 35% and 18%, respectively. After 14 days of injections of CdCl 2 solution the content of GSH in liver and in red blood cells of mice decreased by 32% and 29% respectively, compared to the control group of mice. Fur ther experiments were carried out in order to examine the influence of Cd ions on the content of MDA in liver and in red blood cells of mice after 2 h, 8 h, 24 h and 14 days of in jections of CdCl 2 solution. The results indicate that at the 8 th h and 24 th h MDA content in mice liver was significant ly increased by 236% and 118%, respectively. Meanwhile MDA content in mice red blood cells in all time points was at the control level.

show abstract

Influence of cadmium ions on the antioxidant status and lipid peroxidation in mouse liver: protective effects of zinc and selenite ions

Cited by 8 publications

References 23 publications

Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model

Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model

The comparison of the protective effect of zinc and selenite ions against cadmium induced changes of protein synthesis in mice organs

Influence of cadmium ions on the reduced glutathione and lipid peroxidation in the liver and red blood cells of mice

Contact Info

Product

Resources

About