The aim of this study was to compare the bonding ability of a universal dental adhesive (Scotchbond Universal/SBU, 3 M ESPE) and other contemporary dental bonding agents applied to different substrates: enamel, dentin, resin composite, and porcelain. SBU was tested using both the etch-and-rinse/ER and self-etch/SE bonding approaches. The other adhesives tested were Scotchbond Multipurpose/SBMP (3 M ESPE), Single Bond 2/SB (3 M ESPE), and Clearfil SE Bond/CLSE (Kuraray). Specimens of each substrate were prepared for microtensile bond strength test/μTBS (dentin and composite) or shear/SBS test (enamel and porcelain). In composite and porcelain, negative (no treatment) and positive (silane + SB) control groups were tested. Data were analyzed using One-Way ANOVA and Tukey's test (α = 0.05). In enamel, SBU resulted in similar SBS (p ≥ 0.458) compared to all other adhesives