Influence of Cell Density on Energy Metabolism and the Actin Skeleton of Different Cell Types

Münnich, A.; Bereiter‐Hahn, Jürgen

doi:10.1007/978-3-642-77817-9_32

Cited by 1 publication

(2 citation statements)

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“…In addition, the activation of motility after contact in SV40-3T3 cells that are not contact inhibited agrees with this statement. These findings are in accord with a broad range of changes in cell physiology after reaching confluency, including metabolism and proliferation (Steinberg 1986;Wieser and Oesch 1987;Dulbecco 1970;Castor 1971;Bortell et al 1992;Münnich and Bereiter-Hahn 1993;Siczkowski and Ng 1995).…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Cellular motility in vitro as revealed by scanning acoustic microscopy depends on cell-cell contacts

Zoller

Brändle

Bereiter‐Hahn

1997

Cell and Tissue Research

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A new method is described for observing and quantifying an aspect of contact inhibition of cell movement that is sometimes called "contact paralysis". Based on the scanning acoustic microscope (SAM), the method can detect changes in the mechanical properties of cells, as well as changes in their motility and may therefore be more sensitive to some dynamic changes than methods based on optical microscopy. With this method intracellular motility of normal and transformed cells of epithelial and fibroblastic origin was investigated. By subtraction of SAM images patterns of motility, domains were detected that changed in a characteristic way among various cell lines. Wave-like and nucleating domains could be distinguished; they were also used for the quantification of motility. Like migration intracellular motility is influenced by cell-cell contacts. In zones where a cell touches its neighbours motility domains change or disappear depending on the cell type. In immortalized epithelial cells (XTH-2 cells) large quiescent zones developed in the region where contact with neighbouring cells was established, whereas in fibroblastic (3T3) cells motility was reduced less and did not change in domain pattern. Epithelial and fibroblastic cells were less motile when in contact with other cells or in confluent cultures than when solitary, i.e. their motility was contact inhibited. Transformed (SV40 3T3) cells, however, did not reduce their motility when in contact to or enclosed by other cells. The molecular basis for motility domains remains to be investigated.

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Section: Discussionsupporting

confidence: 83%

“…Increasing cell density in tissue culture reduces energy metabolism in mouse fibroblasts (e.g. Münnich and Bereiter-Hahn 1993) and the turnover number of a Na + /H + exchanger (Siczkowski and Ng 1995).…”

Section: Introductionmentioning

confidence: 99%