Influence of Cellular Density on Determination of EC50 in Microalgal Growth Inhibition Tests

Moreno-Garrido, Ignácio; Lubián, Luís M.; Soares, Amadeu M.V.M.

doi:10.1006/eesa.2000.1953

Cited by 114 publications

(59 citation statements)

References 18 publications

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“…The highest dose of M. oleifera seed extract (64 mg L ), as EC 50 values increase, and thus sensitivity decreases, with higher initial algal densities [41]. This seems further supported by the experiments in [21] where a blooming density of M. aeruginosa…”

Section: Discussionsupporting

confidence: 55%

Effect of Selected Plant Extracts and D- and L-Lysine on the Cyanobacterium Microcystis aeruginosa

Lürling

Oosterhout

2014

Water

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Abstract:We tested extracts from Fructus mume, Salvia miltiorrhiza and Moringa oleifera as well as L-lysine and D-Lysine as curative measures to rapidly suppress the cyanobacterium Microcystis aeruginosa NIVA-CYA 43. We tested these compounds under similar conditions to facilitate comparisons. We hypothesized that for each compound, relatively low concentrations-i.e., 5-50 mg L . F. mume extract was effective to do so at high concentrations, i.e., at ≥240 mg L −1 , but the others were virtually non-effective. Low pH caused by organic acids is a probable explanation for the effect of F. mume extract. No complete wipe-outs of the experimental population were achieved as Photosystem II efficiency showed a recovery after six days. L-lysine may be effective at low concentrations-meaning low material costs. However, the effect of L-lysine seems relatively short-lived. Overall, the results of our study did not support the use of the tested plant extracts and amino-acid as promising candidates for curative application in M. aeruginosa bloom control.

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Section: Discussionsupporting

confidence: 55%

Effect of Selected Plant Extracts and D- and L-Lysine on the Cyanobacterium Microcystis aeruginosa

Lürling

Oosterhout

2014

Water

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“…Green algae and diatoms are widely-used for toxicity assessments (Moreno-Garrido et al 2000). These include Chlorella vulgaris, Closterium ehrenbergii, Ditylum brightwellii, Navicula pelliculosa, Nitzschia closterium, Scenedesmus subspicatus, and Skeletonema costatum.…”

Section: Cell Counting and Chl A Estimationmentioning

confidence: 99%

Evaluation of the sub-lethal toxicity of Cu, Pb, bisphenol A and polychlorinated biphenyl to the marine dinoflagellate Cochlodinium polykrikoides

Ebenezer¹,

2012

ALGAE

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Algae are sensitive to a wide range of pollutants, and are effective bioindicators in ecotoxicity assessments. Here, we evaluated the sub-lethal sensitivity of the marine dinoflagellate Cochlodinium polykrikoides upon exposure to copper (Cu), lead (Pb), bisphenol A (BPA), and Aroclor 1016 (polychlorinated biphenyl, PCB). Toxic effects were assessed by observations of the reduction in cell counts and chlorophyll a levels after exposure to each toxicant. C. polykrikoides displayed dose-dependent, sigmoidal responses when exposed to the tested chemicals. EC 50 -72 h values for Cu, Pb, BPA, and PCB were 12.74, 46.70, 68.15, and 1.07 mg L -1 , respectively. PCB, which is an endocrine-disrupting chemical, was the most sensitive, proving its toxic effect on the dinoflagellate. This study provides baseline data on the toxic effects of commonly used heavy metals and endocrine-disrupting chemicals to a marine dinoflagellate.

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“…Hence, it is important to try to unify the maximum possible different obtained answers, considering aspects such as the influence of initial cellular density in toxicity tests and cellular size. As already established by other authors, such as Moreno-Garrido et al (2000) and Franklin et al (2002), the Cu toxic effect decreases with an increase of the initial cellular density of the bioassay; less Cu is bound to the cells, resulting in less Cu uptake, therefore lower toxicity at higher initial cellular densities. Hence, Cu toxicity results proportional to the amount of Cu associated with the cell at each cell density.…”

Section: Discussionmentioning

confidence: 60%

Toxicity of copper in natural marine picoplankton populations

et al. 2009

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Standard microalgae toxicity tests should be able to establish responses in real ecosystems. Natural marine picoplankton samples collected during the months of March, June, August, October 2007 and January 2008, where exposed to 72 h copper toxicity tests. Results analysed by flow cytometry distinguished two groups, with different cytometric characteristics that can match with two of Synechococcus populations. EC(50) values for these two populations resulted low, ranging from 0.62 to 26.28 microg L(-1), this converts copper in a very powerful contaminant and Synechococcus in one of the most sensitive groups of phytoplankton. Differences in EC(50) values for a same population can be related to the month of collection including different initial cellular densities and oceanographic parameters that can affect the picoplankton's tolerance and distribution.

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Influence of Cellular Density on Determination of EC50 in Microalgal Growth Inhibition Tests

Cited by 114 publications

References 18 publications

Effect of Selected Plant Extracts and D- and L-Lysine on the Cyanobacterium Microcystis aeruginosa

Effect of Selected Plant Extracts and D- and L-Lysine on the Cyanobacterium Microcystis aeruginosa

Evaluation of the sub-lethal toxicity of Cu, Pb, bisphenol A and polychlorinated biphenyl to the marine dinoflagellate Cochlodinium polykrikoides

Toxicity of copper in natural marine picoplankton populations

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