Influence of Chitosan Coating on Mechanical Stability of Biopolymer Carriers with Probiotic Starter Culture in Fermented Whey Beverages

Abstract

The aim of this study was to improve the mechanical stability of biopolymer carriers and cell viability with addition of chitosan coating during fermentation process and product storage. Dairy starter culture (1% (w/v)) was diluted in whey and mixed with sodium alginate solution and the beads were made using extrusion technique. The mechanical stability of coated and uncoated beads, the release behavior, and the viability of encapsulated probiotic dairy starter culture in fermented whey beverages were analyzed. The mechanical properties of the beads were determined according to force-displacement and engineering stress-strain curves obtained after compression testing. It was observed that addition of chitosan as a coating on the beads as well as the fermentation process increased the elastic modulus of the calcium alginate-whey beads and cell survival. The current study revealed that the coating did not significantly improve the viability of probiotics during the fermentation but had an important influence on preservation of the strength of the carrier during storage. Our results indicate that whey-based substrate has positive effect on the mechanical stability of biopolymer beads with encapsulated probiotics.

Microencapsulation of Lactobacillus plantarum 299v incorporated with oligofructose in chitosan coated-alginate beads and its storage stability in ambarella juice

“…as ion-exchange reaction would be taken place when the beads absorb bile salt (Li et al, 2011;Moghtader et al, 2017). Thus, this enhanced the mass transfer resistance and limits the penetration of bile salt into the beads (Obradović et al, 2015). Similar findings were presented by Krasaekoopt et al (2004) and Kamalian et al (2014) whereby the survivability of Lactobacillus casei, Bifidobacterium bifidum and B. pseudocatenulatum G4 entrapped in chitosan-coated alginate beads were higher survivability compared with the uncoated alginate beads.…”

Chitosan Coating Applications in Probiotic Microencapsulation

“…Obradović et al [123] investigated the protection of chitosan coating on cell viability of microencapsulated probiotic starter culture (containing S. salivarius ssp. thermophilus, L. delbrueckii ssp.…”

Potential probiotic characterization and effect of encapsulation of probiotic yeast strains on survival in simulated gastrointestinal tract condition

“…Most of the commercialized probiotics contain bacteria, such as Lactobacillus, Bifidobacterium and Bacillus. [2]. Very few probiotics contain yeast in its composition.…”