Influence of cholesterol and prostaglandin E1 on the molecular organization of phospholipids in the erythrocyte membrane. A fluorescent polarization study with lipid-specific probes

Manevich, E.M.; Lakin, K M; Archakov, Alexander I.; Li, Vladimir S.; Molotkovsky, Julian G.; Безуглов, В. В.; Bergel'son, L. D.

doi:10.1016/0005-2736(85)90373-6

Cited by 21 publications

(2 citation statements)

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“…4) of the lipid probe, although the dissociation constants differed substantially from those derived from radioligand studies with iodinated antibodies. In a similar way it was shown that binding of prostaglandins to erythrocytes or of platelet activation factor (PAF) to platelets prelabeled with AV-lipid probes, resuited in lipid rearrangements detectable by changes in the fluorescence polarization of the probes [17,18]. In both cases the response appeared to be specific (the effects ofprostaglandin E1 and PAF were not paralleled by prostaglandin E2 and chemically modified non-ac- …”

Section: R B Molecules/cellsmentioning

confidence: 95%

Lipid domain reorganization and receptor events Results obtained with new fluorescent lipid probes

Bergel'son¹

1992

FEBS Letters

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Binding of a ligand to the extracellular site of a membrane receptor involves conformational changes not only of the extracellular and cytoplasmic sites but also of the membrane-spanning segments of the receptor protein. The lipids forming the environment of the trans-membrane receptor segments, being flexible and mobile, must follow the shape changes of the receptor molecule to some extent. For this reason ligandreceptor binding itself can be expected to result in changes of the packing of lipid molecules surrounding the receptor. Since lipids are organized in a cooperative manner such changes may affect large areas of the membrane and hence can be registered by physical methods. It has been stated repeatedly that binding of various ligands including hormones, antibodies or lectins to membrane-associated receptors is accompanied by fluidity changes of the target membrane [1][2][3].The aim of this article is to summarize recent evidence suggesting that in highly heterogeneous biological membranes containing numerous metastable lipid domains and/or clusters conformational changes of membrane-associated proteins may result not only in global fluidity changes but also in alterations of the supramolecular lipid domain organization. Frequently such changes can be tbllowed by using, as fluorescent probe, analogs of natural membrane phospholipids or glycolipids bearing, at the end of one of the fatty acyl chains, a 9.anthrylvinyl (AV) group ( cells, and in the latter case may reside for a relatively long time in the plasma membrane without internalization [7].In comparison to most other fluorophores used in membrane research, the AV group displays important advantages. It causes little disturbance because it is nonpolar, flat, and relatively compact. When attached to the fatty acyl chain at an appropriate distance from the head group the AV-fluorophores reside uniformly and exclusively in the center of the bilayer where the host lipids are packed most loosely, and affect neither the mobility nor the orientation and conformation of the major part of the molecules of the surrounding host lipids including their head groups [8]. The AV-group is characterized also by high quantum yields and very short fluorescence lifetimes [9]. The latter property makes the AV-fluorophore suitable for fluorescence polarization measurements. Moreover, in phospholipid bilayers the restricted rotational diffusion of the AVlabeled lipid molecules is of the same time scale as the fluorescence decay. Therefore even subtle changes in the fluidity of the probes microenvironment produce considerable changes in the polarization of fluorescence. The fluorescent parameters of the AV-group (,~ 365 nm, A,~m 430 rim; high extinction coefficient) make it also a highly efficient acceptor for resonance energy transfer (RET) from nearby tryptophans. Since the distance that RET can occur over is as long as 5 nm while RET efficiency depends on the sixth power of the donoracceptor distance, RET measurements with AV-labeled lipids permits the following of even e...

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Section: R B Molecules/cellsmentioning

confidence: 95%

Lipid domain reorganization and receptor events Results obtained with new fluorescent lipid probes

Bergel'son¹

1992

FEBS Letters

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“…Este compuesto modula el estado físico de la membrana al interaccionar con las cadenas hidrocarbonadas de los fosfolípidos y glicolípidos, generando un efecto condensante o "fluidificante" de la bicapa lipídica en función de su temperatura de transición. El incremento de colesterol en la membrana produce aumentos en el parámetro de orden de los componentes de la bicapa lipídica por encima de la temperatura de transición, incrementando la microviscosidad de dicha bicapa, mientras que el descenso de los contenidos de colesterol provoca el efecto contrario, aumenta la fluidez de la membrana (Yeagle, 1985;Kucuk et al, 1992;Vélez et al, 1995;Tirosh et al, 1997;López-Revuelta et al, 2005 (Manevich et al, 1985;López-Revuelta et al, 2007). El efecto modulador del colesterol sobre la estructura y estado físico de la bicapa lipídica de la membrana influye también sobre la movilidad y conformación de las proteínas ancladas en ella, modificando la actividad de canales iónicos, receptores y enzimas (Rooney et al, 1984;Yeagle, 1989;Ohvo-Rekila et al, 2002), lo que puede afectar de un modo determinante al funcionamiento (Yeagle, 1985;Yeagle, 1989;London y Brown, 2000) y a la morfología celular (Pessina et al, 1981;López-Revuelta et al, 2007), alterando en ciertos casos su estabilidad isotónica (Cooper et al, 1975;López-Revuelta et al, 2005;López-Revuelta et al, 2007).…”

Section: Capítulo V: Discusiónunclassified

Efecto de la quercetina y la rutina frente al daño oxidativo inducido en eritrocitos con distintos contenidos de colesterol

Sánchez¹,

Ignácio²

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Efecto de la quercetina y la rutina frente al daño oxidativo inducido en eritrocitos con distintos contenidos de colesterol. Que la presente Memoria de Tesis Doctoral titulada "Efecto de la quercetina y la rutina frente al daño oxidativo inducido en eritrocitos con distintos contenidos de colesterol", de la que es autor D. José Ignacio SÁNCHEZ GALLEGO y que presenta para optar al Grado de Doctor en Biología, ha sido realizada bajo mi dirección en el Departamento de Bioquímica y Biología Molecular de la Universidad de Salamanca. Considero que dicha Tesis Doctoral se halla concluida, y por tanto, autorizo su presentación para que sea juzgada por el Tribunal correspondiente. Y para que así conste, firmo el presente documento en Salamanca a diecisiete de febrero de dos mil nueve.

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Effects of Prostaglandin E ₂ and Progesterone on Rat Brain Synaptosomal Plasma Membranes

Deliconstantinos

2007

Ciba Foundation Symposium 153 ‐ Steroids and Neuronal Activity

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Influence of cholesterol and prostaglandin E1 on the molecular organization of phospholipids in the erythrocyte membrane. A fluorescent polarization study with lipid-specific probes

Cited by 21 publications

References 20 publications

Lipid domain reorganization and receptor events Results obtained with new fluorescent lipid probes

Lipid domain reorganization and receptor events Results obtained with new fluorescent lipid probes

Efecto de la quercetina y la rutina frente al daño oxidativo inducido en eritrocitos con distintos contenidos de colesterol

Effects of Prostaglandin E ₂ and Progesterone on Rat Brain Synaptosomal Plasma Membranes

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Influence of cholesterol and prostaglandin E1 on the molecular organization of phospholipids in the erythrocyte membrane. A fluorescent polarization study with lipid-specific probes

Cited by 21 publications

References 20 publications

Lipid domain reorganization and receptor events Results obtained with new fluorescent lipid probes

Lipid domain reorganization and receptor events Results obtained with new fluorescent lipid probes

Efecto de la quercetina y la rutina frente al daño oxidativo inducido en eritrocitos con distintos contenidos de colesterol

Effects of Prostaglandin E 2 and Progesterone on Rat Brain Synaptosomal Plasma Membranes

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Effects of Prostaglandin E ₂ and Progesterone on Rat Brain Synaptosomal Plasma Membranes