Influence of chronic cadmium exposure on the tissue distribution of copper and zinc and oxidative stress parameters in rats

Erdem, Onur; Yazıhan, Nuray; Koçak, Mukadder; Sayal, Ahmet; Akçıl, Ethem

doi:10.1177/0748233714566875

Cited by 23 publications

(18 citation statements)

References 38 publications

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“…The results of the present studies show that after subchronic administration of a modest dose (0.6 mg/kg SC, 5 days per week for 6–12 weeks), Cd causes significant changes in the renal cortical levels of several key metals, particularly Cu, Se, Fe and Zn. It should be noted that even though many previous studies have shown that Cd can alter renal levels of these metals [11] , [26] , [55] , [53] , there are several aspects of the present studies that are unique and merit special attention.…”

Section: Discussionmentioning

confidence: 94%

Effects of sub-chronic Cd exposure on levels of copper, selenium, zinc, iron and other essential metals in rat renal cortex

2016

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Cd (Cd) is a nephrotoxic environmental pollutant that causes generalized proximal tubule dysfunction. Even though the specific mechanisms by which Cd damages the kidney have yet to be fully elucidated, there is evidence to suggest that some of these nephrotoxic effects may result from the ability of Cd to alter the levels and function of metals such as Cu, Se, Zn and Fe within the kidney. In order to further explore this issue, we examined the effects of subchronic Cd exposure on tissue levels of a panel of metals (Ca, Cu, Fe, K, Mg, Na, Se and Zn) in the rat renal cortex. Adult male Sprague-Dawley rats were treated with CdCl2 (0.6 mg Cd/kg body weight in isotonic saline by subcutaneous injection, 5 days per week for 6, 9 or 12 weeks). At each time point, 24 h urine samples were collected and assayed for levels of protein, creatinine, β2 microglobulin and cystatin C. Samples of renal cortex were removed and assayed for levels of the metals of interest by inductively-coupled mass spectrometry at Michigan State University. Results showed that at 9 and 12 weeks, Cd caused significant increases in urine volume and urinary protein with no change in creatinine excretion. Increases in the excretion of the urinary biomarkers β2 microglobulin and cystatin C were evident after 6 weeks of Cd exposure. Results of the metal analyses showed that Cd caused significant increases in tissue levels of Cu and Se at all of the time points examined. Tissue levels of Zn were transiently elevated at 6 weeks but declined to control levels at 9 and 12 weeks. Cd caused a significant decrease in levels of Fe at 9 and 12 weeks. Cd had no effects on any of the other metals. Tissue levels of Cd were 530 ± 52, 863 ± 23, 837 ± 23 ppm dry weight at 6, 9 and 12 weeks, respectively. These results indicate that the early stages of Cd nephrotoxicity are associated with alterations in renal tissue levels of Cu, Se, Zn and Fe. The fact that the changes in levels of the metals occurred during the early stages of Cd toxicity raises the possibility that the alterations in renal cortical metal content may play some role in the pathophysiology or Cd-induced injury.

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Section: Discussionmentioning

confidence: 94%

Effects of sub-chronic Cd exposure on levels of copper, selenium, zinc, iron and other essential metals in rat renal cortex

2016

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“…Decreased SOD activity in fish exposed to Cd may be related to the reduced availability of bioelements such as Zn or Cu, which are components of SOD, as a result of their binding to metallothionein. Moreover, SOD activity may also be inhibited by the displacement of Mn ions from the active site of MnSOD and Cu and/or Zn ions from CuZnSOD by cadmium (Jurczuk et al 2004 ; Mukherjee et al 2010 ; Erdem et al 2016 ). It is very probable as an increased accumulation of Zn and Cu was noted in our study of the hepatopancreas in fish exposed to the highest concentration of cadmium (Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Cadmium-induced oxidative stress in Prussian carp (Carassius gibelio Bloch) hepatopancreas: ameliorating effect of melatonin

Drąg‐Kozak

Pawlica-Gosiewska

Gawlik

et al. 2019

Environ Sci Pollut Res

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The oxidative status of the hepatopancreas of Prussian carp females ( Carassius gibelio ) co-exposed to sublethal cadmium in water and melatonin was studied. The activities of antioxidant enzymes such as glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) as well as the concentration of reduced glutathione (GSH) were measured in homogenates of the hepatopancreas. Furthermore, concentrations of cadmium (Cd), zinc (Zn), copper (Cu), and iron (Fe) in the hepatopancreas were assayed. These females received melatonin implants and were exposed to 0.4 mg/L or 4.0 mg/L Cd in water for either a 13- or a 7-week period, followed by further 6 weeks of purification in clear water. Exposure to Cd influenced the increase in this metal concentration in fish hepatopancreas. In contrast, the fish exposed to cadmium with additional administration of melatonin had a lower accumulation of this metal. Exposure to Cd caused the increase in GSH content and the activity of GR, and a reduction in GPx activity, whereas the SOD activity varies depending on the exposure time on cadmium. In the hepatopancreas of fish treated with Cd alone, the content of Cu and Zn were increased and that of Fe was changed. After melatonin administration to Cd-exposed fish, a decrease in copper and zinc hepatopancreas content was noted. The present findings imply that melatonin co-treatment can effectively protect the fish against the toxic effects of cadmium on endogenous antioxidant status in hepatopancreas tissues and variations in metal concentration, such as Zn, Cu, and Fe.

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“…Erdem O et al reported that superoxide dismutase activity in liver, heart, and kidney decreased and glutathione peroxidase activity significantly increased accompanied by reduced Cu and Zn after SD rats were given 15mg/L of cadmium chloride orally for 8 weeks. Their results suggest that a high concentration of Cd might affect the concentration of biological elements, such as Cu and Zn, in vivo [38,39]. In addition, a suitable concentration of Zn had an antagonistic effect on the toxicity of Cd.…”

Section: Discussionmentioning

confidence: 99%

Joint Toxicity of a Multi-Heavy Metal Mixture and Chemoprevention in Sprague Dawley Rats

Tang

Zhou

Hua

et al. 2020

IJERPH

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To explore the joint toxicity and bio-accumulation of multi-heavy metals and potential chemoprevention strategies, Male Sprague Dawley (SD) rats (n = 30) were treated orally once a week for six months with 500mg/kg•bw of eight heavy metals which were commonly identified in aquatic products in the Ningbo area including chromium, manganese, nickel, copper, zinc, cadmium, mercury, and lead. At the same time, 200mg/kg•bw of epigallocatechin-3-gallate (EGCG), trisodium citrate dihydrate (TCD) or glutathione (GSH) were administered to evaluate their antagonistic effects against adverse effects of multi-heavy metal mixture. The Morris water maze test was used to evaluate spatial learning and memory in the treated rats. Then the rats were anesthetized by pentobarbital sodium (40 mg/kg•bw) to obtain blood samples for biochemical analysis and organs (heart, liver, spleen, lungs, kidneys, brain, testis) to be conducted for biopsy and organ coefficients. Inductively coupled plasma mass spectrometer (ICP-MS) was used to analyze the concentrations of heavy metals. Results indicated that six months of exposure to a multi-heavy metal mixture under this experimental dosage resulted in accumulation in organs and adverse effects on the blood, reproductive system, and liver function. EGCG, TCD or GSH all showed certain chemoprevention effects against the joint toxicity induced by the multi-heavy metal mixture and indicated alleviation and the potential mechanism that also included the promotion of excretion of metals to which animals were exposed.

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Influence of chronic cadmium exposure on the tissue distribution of copper and zinc and oxidative stress parameters in rats

Cited by 23 publications

References 38 publications

Effects of sub-chronic Cd exposure on levels of copper, selenium, zinc, iron and other essential metals in rat renal cortex

Effects of sub-chronic Cd exposure on levels of copper, selenium, zinc, iron and other essential metals in rat renal cortex

Cadmium-induced oxidative stress in Prussian carp (Carassius gibelio Bloch) hepatopancreas: ameliorating effect of melatonin

Joint Toxicity of a Multi-Heavy Metal Mixture and Chemoprevention in Sprague Dawley Rats

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