Influence of ciprofloxacin on microbial community structure and function in soils

Cui, Hao; Wang, Shuping; Fu, J. S.; Zhou, Zhiqiang; Zhang, Na; Guo, Li

doi:10.1007/s00374-014-0914-y

Cited by 78 publications

(30 citation statements)

References 54 publications

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“…Stimulation was also observed in soil microcosms treated with CIP [92] and NOR [74]. In these experiments, nitrification was stimulated only at the lowest doses tested (1 mg· kg…”

Section: Effects Of Antibiotics On Nitrificationmentioning

confidence: 76%

Antibiotics and the Terrestrial Nitrogen Cycle: A Review

DeVries

Zhang

2016

Curr Pollution Rep

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The distribution, fate, and effects of human and veterinary antibiotics in the environment have been the subject of intense investigation for nearly two decades. Studies show that the structure and function of microbial communities in soil and sediment are modified by antibiotic exposure but the resulting impact on biogeochemical processes is poorly understood. This review summarizes the most recent data on the present use and physicochemical properties of human and veterinary antibiotics and provides an overview of their occurrence in soil and sediment. This is followed by an examination of the potential effects of antibiotics on microbial nitrogen turnover and methodological approaches to measuring the effects of antibiotics on nitrification and denitrification. Recent studies identified six major classes of antibiotics in soil and sediment, occurring at concentrations between ng·kg −1 and mg·kg −1. Among these, tetracycline and fluorquinolone antibiotics are the most resistant to degradation and leaching and may accumulate to high concentrations (mg·kg ) but their occurrence is also reported more frequently.Only 26 studies were found that investigated the effects of antibiotics on microbial nitrogen cycling. Some antibiotics had no observable effect on nitrogen redox activity in soil and sediment while others appeared to increase or decrease rates of reaction. This lack of consensus could be attributed to a number of different variables including antibiotic dose, method of antibiotic application, variations in the microbial community structure, or method of quantification. We conclude by recommending that future studies adopt a more comprehensive approach to report on changes of the microbial community structure and function as well as the short-and long-term impacts of antibiotics on the accumulation and loss of nitrogen pollutants.

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“…Stimulation was also observed in soil microcosms treated with CIP [92] and NOR [74]. In these experiments, nitrification was stimulated only at the lowest doses tested (1 mg· kg…”

Section: Effects Of Antibiotics On Nitrificationmentioning

confidence: 76%

Antibiotics and the Terrestrial Nitrogen Cycle: A Review

DeVries

Zhang

2016

Curr Pollution Rep

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“…Dessa forma, torna-se importante a utilização de técnicas moleculares para efeito de comparação de resultados e validação de metodologias empregadas. Cui et al (2014) em um trabalho que se pesquisou a influência do ciprofloxanino (CIP), um antibiótico da classe das quinolonas, sobre a estrutura e função da comunidade microbiana de um Ustic Cambosol proveniente da província de Hebei, localizada no centro-norte da China, utilizaram cromatografia a gás (GC) para mensurar a atividade do solo por quantificação de CO2 liberado e análise de ácidos graxos derivados de fosfolipídios (PLFAs) para se obter biomassa microbiana, bacteriana e fúngica, e concluíram que o CIP diminuiu significativamente a biomassa microbiana (p<0,05), mas não afetou a atividade/respiração do solo, mesmo em altas doses, em um ensaio com duração de 40 dias. Os mesmos autores confirmaram que o CIP diminuiu a proporção de bactérias para fungos e aumentou a proporção de bactérias Gram-positivas para bactérias Gram-negativas.…”

Section: Resultsunclassified

“…Contaminantes como herbicidas (Reis et al, 2008;Gomez et al, 2009;Tironi et al, 2009;Zhang et al, 2010;Abbas et al, 2014), corantes e compostos fenólicos (Perovano Filho, Silva e López, 2011), metais pesados (Liao et al, 2010;Quadro et al, 2011;Zhang et al, 2013;Muhlbachova et al, 2015), pesticidas (Das, Pal e Chowdhury, 2007;Chowdhury et al, 2008;Imfeld;Vuilleumier, 2012;Portilho et al, 2015;Lv et al, 2017), petróleo e seus derivados (Peña et al, 2007;Guo et al, 2012) e fármacos, com ênfase aos antibióticos (Gao et al, 2013;Cui et al, 2014;Reichel et al, 2014;Ma et al, 2016;Waiser et al, 2016;Molaei et al, 2017) são exemplos de substâncias consideradas estranhas à microbiota do solo que foram estudadas em trabalhos realizados nos últimos quinze anos.…”

Section: Introductionunclassified

Impacto, em curto prazo, dos antibióticos amoxicilina e doxiciclina na qualidade microbiana de um Latossolo Vermelho-amarelo (Short-term impact of the antibiotics amoxicillin and doxycycline on the microbial quality of a Red-yellow Latosol)

Rezende

Kondo

Melloni

2019

Rev. Bras. Geog. Fis.

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Concentrações residuais de antibióticos de uso compartilhado pela terapia médica humana e veterinária são cada vez mais frequentes nos mais variados tipos de matrizes ambientais; no entanto, pouco se sabe sobre o impacto que esses fármacos podem acarretar aos microrganismos do solo. Sendo assim, perturbações relacionadas à exposição da microbiota de um latossolo vermelho-amarelo brasileiro a dois antibacterianos, a amoxicilina (AMOX) e a doxiciclina (DOX), foram investigadas por meio da determinação de atividade (mg CO2) e biomassa (Cmic) microbianas, juntamente com o quociente metabólico (qCO2), em amostras de solo que receberam as seguintes concentrações desses compostos: 0,03, 0,3, 3,0, 30 e 300 mg L-1. Os resultados mostraram diferentes efeitos sobre a microbiota e de forma específica para cada antibiótico. A AMOX mostrou-se mais impactante para os microrganismos do solo, com redução da biomassa e aumento do qCO2, enquanto que a DOX reduziu a atividade microbiana, mas sem efeito na biomassa e qCO2.A B S T R A C TThe residual concentrations of antibiotics used by human and veterinary medical therapy are increasingly common in a wide range of environmental matrices, nevertheless little is known about the impact of these drugs on to the soil microorganisms. Therefore, disturbances related to the exposure of the microbiota of a Brazilian Red-yellow Latosol to two antibacterials, amoxicillin (AMOX) and doxycycline (DOX), were investigated through the determination of the microbial activity (mg CO2) and biomass (Cmic), among with the metabolic quotient (qCO2), using soil samples spiked with: 0,03, 0,3, 3,0, 30 and 300 mg L-1 of each drug. The results showed different effects on the microbiota and in a specific way for each antibiotic. The AMOX showed higher impact impacting for the soil microorganisms, with reduction of the biomass and increase of the qCO2, whereas the DOX reduced the microbial activity, but showed no effect in the biomass and qCO2.Keywords: Antibiotics. Amoxicillin. Doxycycline. Bioindicators. Latosols.

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“…The duration of the exposure of colistin used by Bressan et al (2013) on ammonia-oxidizing bacteria was 5 h. Schmidt et al (2012) already stated that Binhibition of cell division or protein biosynthesis may be observed only after test duration of several days.^The importance of incubation time was also shown in the BSubstrate Induced Respiration (SIR)^test of Thiele-Bruhn and Beck (2005); no effect was observed after 4 h, whereas respiration was inhibited by both oxytetracycline and sulfapyridine after 24 h. Short-term experiments that are shorter than the growth rate of the targeted bacteria, therefore, explore the effect of the antibiotic on enzyme activity. Studies regarding short-term effects (Bressan et al 2013;Costanzo et al 2005;Hou et al 2015;Katipoglu-Yazan et al 2013) can be contrasted to long-term effect studies: from days (Ahmad et al 2014;Alighardashi et al 2009;Conkle and White 2012;Fountoulakis et al 2004;Hou et al 2015;Ingvorsen et al 2003;Thiele-Bruhn and Beck 2005;Yamamura et al 2014), weeks (Campos et al 2001;Cui et al 2014;Klaver and Matthews 1994;Kotzerke et al 2008;Kotzerke et al 2011;Lotti et al 2012;Rico et al 2014;Roose-Amsaleg et al 2013;Toth et al 2011;Underwood et al 2011;Wunder et al 2013;Yan et al 2013), months (Fernandez et al 2009;Hansen et al 1992;Näslund et al 2008;Rosendahl et al 2012), to a year (442 days by Schmidt et al 2012). Conducting experiments for extended time periods is difficult because degradable antibiotics, as well as nutrients, can be quickly consumed unless continuously added …”

Section: Duration Of the Experiments: Short-term Versus Long-term Effmentioning

confidence: 97%

Do antibiotics have environmental side-effects? Impact of synthetic antibiotics on biogeochemical processes

Roose‐Amsaleg

Laverman²

2015

Environ Sci Pollut Res

170

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Antibiotic use in the early 1900 vastly improved human health but at the same time started an arms race of antibiotic resistance. The widespread use of antibiotics has resulted in ubiquitous trace concentrations of many antibiotics in most environments. Little is known about the impact of these antibiotics on microbial processes or "non-target" organisms. This mini-review summarizes our knowledge of the effect of synthetically produced antibiotics on microorganisms involved in biogeochemical cycling. We found only 31 articles that dealt with the effects of antibiotics on such processes in soil, sediment, or freshwater. We compare the processes, antibiotics, concentration range, source, environment, and experimental approach of these studies. Examining the effects of antibiotics on biogeochemical processes should involve environmentally relevant concentrations (instead of therapeutic), chronic exposure (versus acute), and monitoring of the administered antibiotics. Furthermore, the lack of standardized tests hinders generalizations regarding the effects of antibiotics on biogeochemical processes. We investigated the effects of antibiotics on biogeochemical N cycling, specifically nitrification, denitrification, and anammox. We found that environmentally relevant concentrations of fluoroquinolones and sulfonamides could partially inhibit denitrification. So far, the only documented effects of antibiotic inhibitions were at therapeutic doses on anammox activities. The most studied and inhibited was nitrification (25-100 %) mainly at therapeutic doses and rarely environmentally relevant. We recommend that firm conclusions regarding inhibition of antibiotics at environmentally relevant concentrations remain difficult due to the lack of studies testing low concentrations at chronic exposure. There is thus a need to test the effects of these environmental concentrations on biogeochemical processes to further establish the possible effects on ecosystem functioning.

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Influence of ciprofloxacin on microbial community structure and function in soils

Cited by 78 publications

References 54 publications

Antibiotics and the Terrestrial Nitrogen Cycle: A Review

Antibiotics and the Terrestrial Nitrogen Cycle: A Review

Impacto, em curto prazo, dos antibióticos amoxicilina e doxiciclina na qualidade microbiana de um Latossolo Vermelho-amarelo (Short-term impact of the antibiotics amoxicillin and doxycycline on the microbial quality of a Red-yellow Latosol)

Do antibiotics have environmental side-effects? Impact of synthetic antibiotics on biogeochemical processes

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