Influence of co-incubation and cell number of platelets and polymorphonuclear leukocytes in cellular inhibition and activation phenomena

Hernández, R.; Alemany, Mònica; Bozzo, J.; Ordinas, Antonio; Bastida, E

doi:10.1016/0049-3848(94)90113-9

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…The results of our study suggest that hemodialysis procedures can trigger cell cross-talk mechanisms between different cellular elements. We and others have previously demonstrated that eicosanoids, as well as PAF acether, participate in the communication between leukocytes and platelets [37, 38, 39]. However, we believe that efficient communication between platelets and leukocytes is more likely by the activation of specific adhesion molecules mediating interactions between both types of cell, as has been suggested by other authors [10, 40, 41].…”

Section: Discussioncontrasting

confidence: 36%

Platelet-Leukocyte Activation during Hemodialysis Detected with a Monoclonal Antibody to Leukocyte Integrin CD11b

Hernández¹,

Galán²,

Lozano³

et al. 1998

Nephron

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Platelet activation is commonly monitored with a battery of monoclonal antibodies against different platelet epitopes with controversial results. The transient expression of platelet markers and their role mediating interactions with other cells could easily explain these discrepancies. The present study has evaluated whether the analysis of a leukocyte activation antigen (CD11b) could provide more reliable results than detection of platelet activation markers. Cytometric techniques with specific monoclonal antibodies were used to compare the reliability of platelet and leukocyte markers to detect activation. Modifications in the presence of platelet glycoproteins GPIb (CD42b), GPIIIa (CD41) and GPIV (CD36), expression of specific platelet markers (P-selectin (CD62P) and lysosomal protein (CD63)) and leukocyte integrin (CD11b) were assessed during hemodialysis. Platelet antigens remained in uremic patients at levels similar or slightly above those detected in a group of healthy subjects. Modifications of platelet antigens during hemodialysis produced inconclusive results. However, numbers of leukocytes expressing CD11b increased progressively during hemodialysis (17.2 ± 5.1% at 15 min and 21.3 ± 6.6% at 2 h, p < 0.05, vs. baseline 6.9 ± 0.2%). The hemodialysis procedure caused an increased formation of leukocyte-platelet aggregates. Detection of leukocyte CD11b may be a useful marker of overall cellular activation during the hemodialysis procedure.

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Section: Discussioncontrasting

confidence: 36%

Platelet-Leukocyte Activation during Hemodialysis Detected with a Monoclonal Antibody to Leukocyte Integrin CD11b

Hernández¹,

Galán²,

Lozano³

et al. 1998

Nephron

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“…These results suggest that leucocytes or red cells could participate in the mechanism of the action of the drug because the antiaggregatory effect was not observed when platelets were incubated with PRP. In fact, our group [18–21] and others [22–24] have found evidences in recent years of complex interactions between leucocytes and platelets. Moreover, previous studies from our group have also demonstrated that platelet function is also modified by the rheological behaviour of erythrocytes [25,26].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of effects of rofecoxib on platelet function in an in vitro model of thrombosis with circulating human blood

Hernández¹,

Tonda²,

Pino³

et al. 2004

Eur J Clin Investigation

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Function and Clinical Significance of Platelet-Derived Microparticles

Nomura

2001

Int J Hematol

117

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Microparticles released from platelets (PMPs) may play a role in the normal hemostatic response to vascular injury because they demonstrate prothrombinase activity. PMPs were first observed as released vesicles from platelets following adhesion to vessel walls, and flow cytometry is now the most widely used method for studying PMPs. PMPs are thought to play a role in clinical disease because they express phospholipids that function as procoagulants. High shear stress can initiate both platelet aggregation and shedding of procoagulant-containing PMP, suggesting that PMP generation by high shear stress occurs in small diseased arteries and arterioles under various clinical conditions. In addition, the possibility that PMPs evoke cellular responses in their immediate microenvironments has recently been suggested. Despite many interesting findings, the significance of PMPs in various clinical conditions remains controversial. For example, it is not known whether PMPs found in peripheral blood vessels cause thrombosis, or if they are the results of thrombosis. There has been some question about whether the PMPs found in thromboses are consumed locally, meaning that PMPs circulating in the peripheral blood are not functionally important. Currently, the number of clinical disorders associated with elevated PMPs is increasing.

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Influence of co-incubation and cell number of platelets and polymorphonuclear leukocytes in cellular inhibition and activation phenomena

Cited by 5 publications

References 17 publications

Platelet-Leukocyte Activation during Hemodialysis Detected with a Monoclonal Antibody to Leukocyte Integrin CD11b

Platelet-Leukocyte Activation during Hemodialysis Detected with a Monoclonal Antibody to Leukocyte Integrin CD11b

Evaluation of effects of rofecoxib on platelet function in an in vitro model of thrombosis with circulating human blood

Function and Clinical Significance of Platelet-Derived Microparticles

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