Platelet-Leukocyte Activation during Hemodialysis Detected with a Monoclonal Antibody to Leukocyte Integrin CD11b

Hernández, Marı́a Rosa; Galán, Ana M.; Lozano, Miguel; Bozzo, J.; Cases, Aleix; Escolar, Ginés; Calls, J.; Ordinas, Antonio

doi:10.1159/000045167

Cited by 20 publications

(18 citation statements)

References 24 publications

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“…In previous studies, we have already evaluated the biocompatibility of dialysis membranes. Results from those studies confirm previous data indicating that different membranes have variable ability to activate platelets and leukocytes assessed by differences in the percentages of heterotypic aggregates formed and by a priming state observed in leukocytes of hemodialysis patients [3, 26]. In contrast, the cellular adhesion process is not dependent on the type of dialysis membrane, as no differences were observed between a cellulosic and a synthetic membrane.…”

Section: Discussionsupporting

confidence: 86%

“…Leukocyte activation and heterotypic aggregation to recruit more leukocytes in the area have been previously reported [1, 3, 30]. The present results show that the aggregating response depends on the leukocyte subpopulation studied.…”

Section: Discussionsupporting

confidence: 77%

“…The contact of blood with artificial surfaces may activate blood leukocytes and platelets and initiate the leukocyte inflammatory response. Moreover, activated platelets may adhere to leukocytes to form heterotypic platelet-leukocyte aggregates [3,4,5,6,7], which probably initiate leukocyte-mediated inflammatory responses, leading to other clinical problems currently associated with hemodialysis [8, 9]. Mechanism for the formation of cell-cell interactions (heterotypic aggregates) are primarily related to the expression of adhesion receptors that facilitate cross-talk among different cell lineages: platelets andleukocytes or endothelial cells (ECs) or leukocytes and ECs.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Two Different Dialysis Membranes on Leukocyte Adhesion and Aggregation

Hernández¹,

Palomo²,

Fusté³

et al. 2007

Nephron Clin Pract

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Aims: We evaluated modifications in formation of heterotypic platelet-leukocyte aggregation induced by dialysis through cellulosic or synthetic membranes and evaluated the effects of such procedures promoting adhesive interactions between leukocytes and normal endothelial cells (ECs). Methods: Samples were obtained from arterial and venous lines at baseline, after 15 and 120 min of hemodialysis. Heterotypic aggregation was assessed using flow-cytometric techniques. Experiments to determine leukocyte adhesion to ECs were performed in parallel plate perfusion chambers at 450 s^–1. Results: Patients dialyzed with a cellulosic membrane showed a significantly higher baseline granulocyte heterotypic aggregation (median 22.5%, range 8.6–32%) versus healthy subjects (median 10%, range 3.2–14.6%; p < 0.05). Granulocyte heterotypic aggregation values remained increased throughout the hemodialysis session not only in the arterial line (median 18 and 24.5%, range 7–30 and 8.7–36% at 15 and 2 h, respectively) but also in the venous line (median 20 and 25%, range 8.6–32 and 11.5–35% at 15 min and 2 h, respectively). Basal lymphocytes heterotypic aggregation values observed in uremic patients were 6% (0.1–7.1%) versus 1.0% (0.5–2.8%) in the control group (p < 0.05). The increase remained during the hemodialysis session both in the arterial line (median 5 and 4%, range 0.2–14 and 0.5–7.1 % at 15 min and 2 h, respectively) and in the venous line (median 7 and 7%, range 1.4–14 and 0.5–10.6% at 15 min and 2 h). In contrast, patients dialyzed with a synthetic membrane showed a decreased basal granulocyte heterotypic aggregation compared to healthy subjects (median 3.5 vs. 10%, range 2.8–7 vs. 3.2–14.6%, respectively; p < 0.05). For lymphocytes, basal heterotypic aggregation values were 0.2% (range 0.1–0.5%) in dialyzed patients vs. 0.98% (range 0.5–2.8%) in healthy subjects (p < 0.05), without changes throughout the dialysis session. Changes in leukocyte adhesion during hemodialysis did not reach statistical significance with either hemodialysis membrane. Our studies confirm a differential activation of platelets and leukocytes depending on the nature of the dialysis membranes. However, activation of circulating cellular elements by hemodialysis procedures did not enhance cross-talk interactions between leukocytes and unaltered ECs.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Two Different Dialysis Membranes on Leukocyte Adhesion and Aggregation

Hernández¹,

Palomo²,

Fusté³

et al. 2007

Nephron Clin Pract

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“…17,31,32 In addition, HD membrane materials may activate neutrophils 33 and platelets. 34 The abrupt change of hemodynamic and metabolic balance caused by dialysis may also add to the development of OS. Further research should be planned in order to accurately measure OS in time intervals between and during dialysis as well as following various time courses and dialysis therapy schemes.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Modulation Immediately After Hemodialysis

Alamdari

Honarmand

Sarrafnejad

et al. 2009

Dialysis & Transplantation

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AIMThe aim of this study was to estimate oxidative stress (OS) before and after dialysis in hemodialysis (HD) patients and correlate this stress with routine biochemical parameters.METHODSera of patients (n = 21), under regular HD, were collected 5 minutes before and after an HD session. Oxidative stress was estimated using the pro‐oxidant‐antioxidant assay (PAB), along with routine biochemical parameters in the same sera.RESULTSA significant increase of OS value was observed in HD patients 5 minutes after dialysis. Before HD, a significant correlation was established between the PAB values and fasting blood sugar, calcium, and C‐reactive protein (CRP); and an inverse correlation between the PAB values and uric acid, serum creatinine, albumin, and albumin/globulin ratio. This correlation was not obvious after HD. PAB value, after dialysis, correlated significantly only with serum iron and inversely with alanine transaminase.CONCLUSIONThis study found that the already‐increased OS in HD patients is further increased immediately after dialysis. However, factors influencing post‐dialysis OS may be different from pre‐dialysis factors.

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“…16 In previous studies we have demonstrated leukocyte activation in uremic patients. 2,17 In the present study, we have explored the relationship between expression of CD11b, leukocyte adhesion and the phosphorylation status of tyrosine, serine and threonine residues of CD11b in leukocytes from uremic patients at baseline and throughout haemodialysis with a cellulose membrane.…”

mentioning

confidence: 99%

Haemodialysis through a cellulose membrane induces dephosphorylation of CD11b and promotes leukocyte adhesion to endothelial cells

Hernández

Fusté²,

Cases³

et al. 2009

CIM

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Clin Invest Med 2009; 32 (1): E48-E56. AbstractPurpose: To explore modifications in signal mechanisms involving CD11b and leukocyte adhesion in patients under haemodialysis (HD). Methods: Samples were obtained from uremic patients at baseline, 15 and 120 min of HD from both arterial and venous lines. CD11b expression was studied by flow cytometry. To study signalling mechanisms, CD11b was immunoprecipitated using a specific antibody. Immunoprecipitates were resolved by 8% SDS-PAGE to measure phosphorylation in immunoblots. Leukocyte adhesion was measured after blood perfusion using endothelial cells (EC) as adhesive substrate. Parallel studies were performed with blood from healthy donors. Results: The percentage of CD11b+ cells increased during HD with a cellulose membrane in the venous line at 15 and 120 min (6.2±2.9% and 11.0±7.1%) and in the arterial line at 120 min (11.5±8.5 vs. 3.1±1.0% in control P<0.05). After 120 min HD, CD11b phosphorylation decreased in leukocytes from both arterial (72.6±2.9) and venous lines (51.8±6.5) vs. basal samples (119.5±15.5 P <0.005). Control leukocytes showed enhanced adhesion to uremic EC compared with control EC (3.0±0.3 vs. 2.3±1.0 leukocytes x100 EC -1 P<0.05). Uremic leukocyte adhesion was enhanced after HD compared with basal samples 4.2±0.2 leukocytes/100 EC in the arterial and 4.4±0.3 in the venous line; after 120 min vs 2.3±1.0 (P<0.005). Conclusion:Leukocyte activation during HD through a cellulose membrane occurs with decreases in CD11b phosphorylation. Activation also induces increases in CD11b expression associated with enhanced leukocyte adhesion to uremic endothelial cells.

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Platelet-Leukocyte Activation during Hemodialysis Detected with a Monoclonal Antibody to Leukocyte Integrin CD11b

Cited by 20 publications

References 24 publications

Effect of Two Different Dialysis Membranes on Leukocyte Adhesion and Aggregation

Effect of Two Different Dialysis Membranes on Leukocyte Adhesion and Aggregation

Oxidative Stress Modulation Immediately After Hemodialysis

Haemodialysis through a cellulose membrane induces dephosphorylation of CD11b and promotes leukocyte adhesion to endothelial cells

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