Influence of Cocaine and Lidocaine on Human Nasal Cilia: Beat Frequency and Harmony In Vitro

Ingels, K.J.A.O.; Nijziel, Marten R.; Graamans, K.; Huizing, Egbert H.

doi:10.1001/archotol.1994.01880260067012

Cited by 38 publications

(28 citation statements)

References 16 publications

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“…Moreover, we cannot determine the duration of contact of vector with the epithelium, which our unpublished studies indicate is an important determinant of the efficiency of gene transfer. In our first study, mucociliary clearance was probably inhibited by the use of topical cocaine and tetracaine (30).…”

Section: Measurement Of Nasal V T In Cf and Non-cf Subjects The Lossmentioning

confidence: 81%

Repeat administration of an adenovirus vector encoding cystic fibrosis transmembrane conductance regulator to the nasal epithelium of patients with cystic fibrosis.

Zabner¹,

Ramsey²,

Meeker³

et al. 1996

J. Clin. Invest.

253

137

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Section: Measurement Of Nasal V T In Cf and Non-cf Subjects The Lossmentioning

confidence: 81%

Repeat administration of an adenovirus vector encoding cystic fibrosis transmembrane conductance regulator to the nasal epithelium of patients with cystic fibrosis.

Zabner¹,

Ramsey²,

Meeker³

et al. 1996

J. Clin. Invest.

253

137

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“…However, in these patients, cocaine was omitted because the 10% cocaine solution used has been shown to cause a severe and prolonged decrease in MTR. 14,15 No substitute was used, and immediately after amputation, the turbinates were submerged in Eagles minimal essential medium (EMEM) and maintained at 37°C in an incubator. Within an hour of its removal, the tissue sample was placed in a double-walled beaker where the temperature was controlled at 37 ( 2°C by running thermostatically controlled water through the chamber.…”

Section: Introductionmentioning

confidence: 99%

Chitosan as a Nasal Delivery System: The Effect of Chitosan Solutions on in Vitro and in Vivo Mucociliary Transport Rates in Human Turbinates and Volunteers

Aspden¹,

Mason²,

Jones³

et al. 1997

Journal of Pharmaceutical Sciences

238

105

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In these studies, we examined the effect on mucociliary transport rates (MTR) of various 0.25% (w/v) chitosan solutions applied to human nasal tissue both ex vivo and in vivo. In the first study a range of chitosans with different molecular weights were applied to freshly amputated human nasal turbinates, and their effect on MTR was recorded. The transient inhibitory effect on turbinate MTR that was found for most of the chitosan preparations showed a marked dependence on the volume of chitosan solution applied and the molecular weight of the chitosan tested. The higher the molecular weight of the chitosan and the more chitosan applied, the longer the original MTR was depressed. A small scale human trial, investigating the effect of chitosan glutamate, on saccharin clearance times, was also undertaken. The study showed that a once daily application of a 0.25% solution of the chitosan for 7 days had no effect on either saccharin clearance times or nasal histology as examined by light microscopy.

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“…Because of the ready accessibility of the upper respiratory tract, most investigators use biopsies from the nasal mucosa. It is, however, difficult to obtain sufficient quantities of healthy mucosa for experimental purposes [3,4]. Cryopreservation of ciliated mucosa has been used only occasionally, but observations on ciliary motility after cryopreservation seem to be encouraging [11].…”

Section: Discussionmentioning

confidence: 99%

Ciliary beat frequency of human sphenoid sinus mucosa after cryopreservation

Boek¹,

Graamans²,

Huizing³

1998

European Archives of Oto-Rhino-Laryngology

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In vitro studies of human ciliary activity require relatively large quantities of specimens of healthy ciliated epithelium. For this reason we investigated whether cryopreserved healthy mucosa taken from the sphenoid sinus during pituitary surgery would meet the demands of this type of study. The sinus mucosa from ten patients was immersed in two different cryopreservatives. One solution contained 10% dimethylsulfoxide (DMSO) as cryoprotector. The other contained glycerol as a part of human sperm preservation medium (HSPM). The ciliary beat frequency (CBF) was measured sequentially by a photoelectrical method: when specimens were fresh and then at intervals of 1 week, 1 month and 3 months after cryopreservation in liquid nitrogen and thawing. Mean CBF values recorded after thawing did not differ significantly from the values measured before cryopreservation. Prior to cryopreservation and after thawing, CBF did not change during a period of 4 h. Epithelia preserved in DMSO demonstrated that the low mean CBF (5.4 Hz) found was caused by a reversible ciliostatic effect of the medium. After thawing and rinsing with a neutral medium, CBF showed normal values. We conclude that sphenoid sinus mucosa is an appropriate source of ciliated mucosa for in vitro experiments. Since non-pathological ciliated epithelium can be maintained in a "mucosa bank," our finding makes further studies of CBF of normal human respiratory epithelium in vitro a realistic goal.

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Influence of Cocaine and Lidocaine on Human Nasal Cilia: Beat Frequency and Harmony In Vitro

Cited by 38 publications

References 16 publications

Repeat administration of an adenovirus vector encoding cystic fibrosis transmembrane conductance regulator to the nasal epithelium of patients with cystic fibrosis.

Repeat administration of an adenovirus vector encoding cystic fibrosis transmembrane conductance regulator to the nasal epithelium of patients with cystic fibrosis.

Chitosan as a Nasal Delivery System: The Effect of Chitosan Solutions on in Vitro and in Vivo Mucociliary Transport Rates in Human Turbinates and Volunteers

Ciliary beat frequency of human sphenoid sinus mucosa after cryopreservation

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