Influence of collagen‐fibril‐based coatings containing decorin and biglycan on osteoblast behavior

Abstract

Collagen is used as a scaffold material for tissue engineering as well as a coating material for implants with a view to enhancing osseointegration by mimicry of the bone extracellular matrix in vivo. The biomimicry strategy can be taken further by incorporating the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, which are expressed in bone. Both bind to fibrils during fibrillogenesis in vitro. In this study, the ability of collagen types I, II, and III to bind decorin and biglycan was compared. Collagen type II bound significantly more SLRPs in fibrils than collagen I and III, with more biglycan than decorin bound by all three collagen types. Therefore, type II fibrils with bound decorin or biglycan or neither were used to coat titanium surfaces. Bioavailability of SLRPs was confirmed by direct ELISA after SLRP biotinilation. The in vitro behavior of osteoblasts from rat calvaria (rOs) and human knee (hOs) cultured on different surfaces was compared. Proliferation and collagen synthesis were determined. Also, the influence of SLRPs on the formation of focal adhesions by rO was investigated. Biglycan enhanced the formation of focal adhesions after 2 and 24 h. Decorin and biglycan affected rO and hO proliferation and collagen synthesis differently. Biglycan stimulated hO proliferation significantly but had no effect on rO proliferation, and also inhibited rO collagen synthesis significantly while not affecting hO collagen synthesis. Decorin promoted hO proliferation slightly but did not influence rO proliferation. The results could be relevant when designing implant coatings or tissue engineering scaffolds.

Preferential cell response to anisotropic electro-spun fibrous scaffolds under tension-free conditions

“…Arrows indicate the orientation of the substrate topography to a specific location. Glycosaminoglycans and proteoglycans [95][96][97] or bioactive molecules such as growth factors or hormones are traditionally used to enhance biological functions of biomaterials [98][99][100][101]. However, such molecules are typically labile; the biologic half-lives of plateletderived growth factor, basic fibroblast growth factor, and vascular endothelial growth factor are 2, 3, and 50 min, respectively, when intravenously administered [102].…”

Control of Collagen Molecular Assembly with Anionic Polysaccharides

“…Douglas et al have shown the ability of several collagens to bind small leucine-rich proteoglycans and glycosaminoglycans (GAGs), and these interactions were not the same. [1][2][3] Tsai et al have shown that polysaccharides can be used to vary the reconstitution rate, fiber size, and morphology of collagen. 4) Human hematopoietic stem cells have been cultivated on collagen-coated film with heparin and with hyaluronic acid.…”

Magnesium substitution effect on porous scaffolds for bone repair

“…Composites in the present study used a polymeric matrix of natural COL not only to achieve a stable composite and to prevent rapid release of calcium ions in surrounding medium [20], but to improve the interaction with osteoblast cells. It is known that COL has binding sites that promote cell attachment through focal contacts and adhesion plaques, providing an increased cell adhesion capacity to composites designed for bone regeneration [35].…”